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[{"key": "dc.contributor.advisor", "value": "Hulmi, Juha", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Permi, Perttu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "M\u00e4ntyselk\u00e4, Sakari", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-06-03T06:17:05Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-06-03T06:17:05Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/76173", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Osaa liikunnan terveyshy\u00f6tyjen mekanismeista on vaikeaa tutkia ihmisill\u00e4 tai koe-el\u00e4imill\u00e4. Viljeltyjen C2C12 myotuubien s\u00e4hk\u00f6pulssistimulaatiolla (EPS, engl. electrical pulse stimulation) voidaan mallintaa lihassupistuksia ja liikuntaa in vitro \u2013olosuhteissa. EPS:n vaikutusta C2C12 myotuubien aineenvaihduntaan ei kuitenkaan tunneta tarkasti. Lis\u00e4ksi C2C12 myotuubeja viljell\u00e4\u00e4n yleens\u00e4 korkeassa ei-fysiologisessa 25 mM glukoosipitoisuudessa, mik\u00e4 voi vaikuttaa EPS-vasteisiin. T\u00e4ss\u00e4 tutkimuksessa C2C12 myotuubeja s\u00e4hk\u00f6pulssistimuloitiin 24 tunnin ajan sek\u00e4 matalan 5.5 mM (MG) ett\u00e4 korkean 25 mM (KG) glukoosipitoisuuden olosuhteissa, ja aineenvaihduntatuotteiden konsentraatiot analysoitiin sek\u00e4 soluista ett\u00e4 elatusaineesta ydinmagneettisella resonanssispektroskopialla (NMR). EPS:n vaikutuksesta, solut k\u00e4yttiv\u00e4t enemm\u00e4n glukoosia sek\u00e4 erittiv\u00e4t paljon laktaattia ja asetaattia molemmissa glukoosipitoisuuksissa, mutta etenkin KG-olosuhteissa. Lis\u00e4ksi etenkin KG-olosuhteissa C2C12 myotuubit erittiv\u00e4t haaroittuneita lyhytketjuisia rasvahappoja (BSCFA) ja ketoaine 3-hydroksibutyraattia. Edelleen havaittiin, ett\u00e4 BSCFA:t korreloivat vahvasti haaraketjuisten aminohappojen (BCAA) kanssa myotuubeissa. Lis\u00e4ksi BSCFA:n m\u00e4\u00e4r\u00e4 laski dramaattisesti, kun C2C12 myotuubeja kasvatettiin BCAA-vapaissa olosuhteissa. N\u00e4in ollen BSCFA:t olivat todenn\u00e4k\u00f6isesti per\u00e4isin BCAA-hajotuksesta ja mahdollisesti asyylikoentsyymi A tioesteraasi 9 -entsyymin (ACOT9) toiminnasta. Tulosten pohjalta voidaan todeta, ett\u00e4 tulevissa tutkimuksissa olisi t\u00e4rke\u00e4\u00e4 ottaa elatusaineen ravintoaineiden m\u00e4\u00e4r\u00e4t paremmin huomioon. Jatkotutkimuksissa olisi hy\u00f6dyllist\u00e4 selvitt\u00e4\u00e4 my\u00f6s asetaatin ja BSCFA:n roolia kudosten v\u00e4lisess\u00e4 aineenvaihdunnallisessa viestinn\u00e4ss\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Some of the mechanisms behind the favorable effects of physical activity are difficult to study in vivo. Electrical pulse stimulation (EPS) of the cultured myotubes has become a widely used in vitro model to mimic in vivo muscle contractions and exercise. However, its detailed effects on metabolic processes are still poorly known. The C2C12 myotubes are usually cultured in supraphysiological 25 mM glucose conditions, which can affect EPS responses. In this present study, C2C12 myotubes were electrical pulse stimulated for 24 hours in low (5.5 mM) and high (25 mM) glucose (LG and HG respectively) conditions, and both intra- and extracellular metabolites were analyzed using untargeted nuclear magnetic resonance (NMR) spectroscopy. In response to EPS, the C2C12 myotubes increased glucose consumption and secreted large amounts of lactate and acetate in both glucose conditions but especially in HG condition. In addition, the C2C12 myotubes secreted branched short-chain fatty acids (BSCFAs) and a ketone body 3-hydroxybutyrate in response to EPS, especially in HG condition. Intracellular BSCFAs and branched-chain amino acids (BCAAs) correlated highly and BSCFA content substantially decreased when myotubes were cultured in BCAA-free medium, suggesting that BSCFAs were formed through the catabolism of BCAAs and possibly by acyl-CoA thioesterase 9 (ACOT9) enzyme. The results suggest that nutrient availability is an important factor to consider in future studies. Further, the role of acetate and BSCFAs in inter-organ metabolic communication would be beneficial to study in the future.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-06-03T06:17:05Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-06-03T06:17:05Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "54", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "ACOT9", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "BSCFAs", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "exercise model", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "metabolomics", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "skeletal muscle", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The metabolic responses to electrical pulse-induced contractions in C2C12 myotubes are greater in high glucose medium", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202106033403", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solumallit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aineenvaihdunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihakset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aminohapot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rasvahapot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glukoosi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell models", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "metabolism", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "muscles", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "amino acids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fatty acids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glucose", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "", "language": null, 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