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[{"key": "dc.contributor.advisor", "value": "Kuopio, Teijo", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ahtiainen, Maarit", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Tynkkynen, Niko", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-05-21T04:41:01Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-05-21T04:41:01Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/75830", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Keuhkosy\u00f6p\u00e4 on maailman yleisin sy\u00f6p\u00e4kuolemien aiheuttaja. Histologisesti se jaetaan pienisoluiseen (15 %) ja ei-pienisoluiseen (85 %) keuhkosy\u00f6p\u00e4\u00e4n (engl. non-small cell lung cancer, NSCLC). ROS1-geenin uudelleenj\u00e4rjest\u00e4ytym\u00e4t ovat harvinaisia NSCLC:ss\u00e4 (1\u20132 %), mutta t\u00e4rkeit\u00e4 tunnistaa kohdennettua hoitoa varten. Immunohistokemia (IHK) ja fluoresenssi in situ hybridisaatio (FISH) ovat yleisi\u00e4 menetelmi\u00e4 tutkittaessa ROS1-uudelleenj\u00e4rjest\u00e4ytymi\u00e4. Potilaat, joilla ei ole ROS1-uudelleenj\u00e4rjest\u00e4ytymi\u00e4, voidaan sulkea pois IHK-seulonnalla. FISH:ta tarvitaan positiivisten IHK-tulosten varmistuksessa. Tavoitteena oli selvitt\u00e4\u00e4 ROS1-uudelleenj\u00e4rjest\u00e4ytymien esiintyvyys suomalaisessa NSCLC-aineistossa ja verrata IHK:aa ja FISH:ta ROS1-analytiikassa. Monikudosblokit koostuivat 578 leikattujen potilaiden kasvainn\u00e4ytteist\u00e4 (319 adenokarsinoomaa, 235 levyepiteelikarsinoomaa, 24 tarkemmin m\u00e4\u00e4rittelem\u00e4t\u00f6nt\u00e4 NSCLC:\u00e4\u00e4), jotka saatiin kahdesta suomalaisesta biopankista. Formaliinilla fiksoidut ja parafiiniin valetut leikkeet v\u00e4rj\u00e4ttiin ROS1-vasta-aineella. IHK:n tulos oli negatiivinen 548 (94,81 %), positiivinen kahdessa n\u00e4ytteess\u00e4 (0,35 %) ja ep\u00e4selv\u00e4 28 (4,84 %) n\u00e4ytteess\u00e4. Positiiviset IHK-tulokset olivat positiivisia FISH:ssa tai toisen sukupolven sekvensoinnissa ja ep\u00e4selv\u00e4t IHK-tulokset olivat negatiivisia FISH:ssa. ROS1-uudelleenj\u00e4rjest\u00e4ytymien esiintyvyys oli odotettua pienempi (0,35 %, adenokarsinoomissa 0,63). ROS1-IHK:n herkkyys (100 %) ja tarkkuus (96 %) vastasivat hyvin aiempia tuloksia. Vain \nmuutamat IHK-n\u00e4ytteet n\u00e4ytt\u00e4v\u00e4t tarvitsevan FISH-varmistuksen.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Lung cancer is the leading cause of cancer death worldwide. Histologically, it is divided into small cell (15 %) and non-small cell (85 %) lung cancer (NSCLC). Rearrangements of ROS1 gene are rare (1\u20132 % of NSCLC), but essential to recognize for targeted therapy. Two common methods to examine ROS1 rearrangements are immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). The patients without ROS1 rearrangements can be ruled out by IHC screening. FISH is needed to confirm positive IHC results. This study aimed to examine the prevalence of ROS1 rearrangements among a Finnish NSCLC material and to compare IHC and FISH in ROS1 analysis. Tissue microarrays of 578 samples (319 adenocarcinomas, 235 squamous cell carcinomas and 24 NSCLC-not otherwise specified) were obtained from two Finnish \nbiobanks. Only surgically operated patients were included in the material. Formalin fixed and paraffin embedded sections were stained using a ROS1 antibody. IHC was negative in 548 (94.81 %), positive in 2 (0.35 %) and equivocal in 28 (4.84 %) samples. The IHC positive samples were positive in FISH or NGS and equivocal samples were negative in FISH. The prevalence of ROS1 rearrangements was 0.35 % (0.63 % among adenocarcinomas) being less \nthan expected. The sensitivity and specificity of ROS1 IHC were 100 % and 96 % corresponding well with previous results. Only few IHC samples seems to need FISH confirmation.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-05-21T04:41:01Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-05-21T04:41:01Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "50", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "molecular diagnostics", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tyrosine kinase", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "ROS1 gene rearrangements in non-small cell lung cancer : immunohistochemistry and fluorescence in situ hybridization", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202105213091", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.collaborator", "value": "public", "language": "", "element": "contractresearch", "qualifier": "collaborator", "schema": "yvv"}, {"key": "yvv.contractresearch.funding", "value": "6600", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "student", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "translokaatio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "keuhkosy\u00f6p\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "adenokarsinooma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "okasolusy\u00f6p\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistokemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "histologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "translocation (genetics)", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancerous diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pulmonary cancer", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "adenocarcinoma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancer cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "squamous cell carcinoma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistochemistry", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "histology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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