| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Vehni\u00e4inen, Eeva-Riikka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kinnunen, Kirsikka", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-03-16T11:30:49Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-03-16T11:30:49Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/74652", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Beta-sitosteroli on puissa luontaisesti esiintyv\u00e4 estrogeeninen yhdiste, jota p\u00e4\u00e4tyy vesist\u00f6ihin sellu- ja paperiteollisuuden j\u00e4tevesien mukana. Sen vaikutuksia on aiemmin tutkittu p\u00e4\u00e4asiassa aikuisilla kaloilla, joten haluttiin selvitt\u00e4\u00e4 mahdollisia vaikutuksia vastakuoriutuneisiin kalanpoikasiin. Koelajina tutkimuksessa oli Suomessa luontaisesti esiintyv\u00e4 j\u00e4rvitaimen (Salmo trutta lacustris), joka voisi k\u00e4rsi\u00e4 beta-sitosterolista. Tutkimuksessa selvitettiin, onko beta-sitosterolilla vaikutusta geenien sytokromi P4501A (cyp1a), estrogeenireseptori alfa (er\u03b1) ja vitellogeniini (vtg) ilmentymiseen poikasissa. Lis\u00e4ksi vaikutuksia poikasten pituuksiin ja ruskuaispussin pinta-aloihin tutkittiin. Er\u03b1 ja vtg ilment\u00e4v\u00e4t aineen estrogeenista vaikutusta ja cyp1a dioksiinityyppist\u00e4 toksisuutta. Tutkimuksessa suoritettiin kaksi altistuskoetta pituuksiltaan 24 h ja 14 vrk. Beta-sitosterolin pitoisuudet olivat 1, 10, 100 ja 1000 \u03bcg/l, sek\u00e4 positiivisena kontrollina toimi reteeni (100 \u03bcg/l). Reteenin liuotinkontrollina oli DMSO (10 \u03bcg/l) ja beta-sitosterolin liuotinkontrollina etanoli (20 ja 200 \u03bcg/l). Kummassakin kokeessa oli kolme toistoa kustakin pitoisuudesta, sek\u00e4 12 poikasta jokaisessa 24 vuoassa. 24 h n\u00e4ytteet tutkittiin reaaliaikaisen polymeraasiketjureaktion (qPCR) avulla, joka kertoo geenin l\u00e4hetti-RNA:n m\u00e4\u00e4r\u00e4n ja siten geenin ilmenemisen. Beta-sitosterolilla ei havaittu vaikutusta tutkittuihin geeneihin, eik\u00e4 pituuksiin ja ruskuaispussien pinta-aloihin. Johtop\u00e4\u00e4t\u00f6ksen\u00e4 voidaan todeta, ettei beta-sitosteroli aiheuta dioksiinin kaltaista toksisuutta j\u00e4rvitaimenen poikasissa, eik\u00e4 sill\u00e4 n\u00e4ytt\u00e4isi my\u00f6sk\u00e4\u00e4n olevan estrogeenivaikutusta vastakuoriutuneisiin j\u00e4rvitaimeniin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Beta-sitosterol is an estrogenic compound which naturally occurs in trees. Pulp and paper mill effluents are major sources of beta-sitosterol from wood to water systems. Effects of beta-sitosterol have mainly been studied by using adult fish, so in this study objects of interests are possible effects on newly hatched fish larvae. The test species in the study was brown trout (Salmo trutta lacustris) that is a native species in Finland and may suffer from beta-sitosterol. In this survey we tested could beta-sitosterol affect the number of transcripts of cytochrome P4501A (cyp1a), estrogen receptor alpha (er\u03b1) and vitellogenin (vtg) in larvae. It was also investigated whether beta-sitosterol affect the lengths and yolk sac areas of larvae. Er\u03b1 and vtg express an estrogenic effect of compound and cyp1a dioxin-like toxicity. In this study we had two experiments: 24 hours long and 14 days long. The concentrations of beta-sitosterol were 1, 10, 100 and 1000 \u03bcg/L and the positive control was retene (100 \u03bcg/L). Solvent control of retene was DMSO (10 \u03bcg/L) and solvent control of beta-sitosterol was ethanol in concentrations 20 and 200 \u03bcg/L. There were three replicates of each solution and in one test experiment there were 24 glass bowls and in each of these were 12 larvae. 24 hours samples were analyzed by using quantitative polymerase chain reaction (qPCR). Amounts of messenger RNA (mRNA) of different genes tell us the activity of the expression of these genes in the cells. Beta-sitosterol did not have any effects on the genes studied nor on the lengths and yolk sac areas. In conclusion it can be said that beta-sitosterol has no dioxin-like or estrogenic effects on newly hatched brown trout larvae.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-03-16T11:30:49Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-03-16T11:30:49Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "57", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "estrogeenireseptori alfa", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sytokromi P4501A", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vitellogeniini", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Beta-sitosterolin vaikutus j\u00e4rvitaimenen (Salmo trutta lacustris) poikasiin", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202103161994", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Akvaattiset tieteet", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Aquatic sciences", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4012", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "selluteollisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "paperiteollisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "poikaset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kalat", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "j\u00e4tevesi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "vaikutukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "j\u00e4rvitaimen", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "polymeraasiketjureaktio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|