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[{"key": "dc.contributor.advisor", "value": "Takala, Heikki", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lehtinen, Kimmo", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-03-08T08:38:10Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-03-08T08:38:10Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/74517", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetiikka mahdollistaa mink\u00e4 tahansa solun toiminnon kontrolloimisen valon avulla. Yksi mielenkiintoinen optogenetiikan k\u00e4ytt\u00f6kohde on apoptoosin laukaiseminen, jota voidaan hy\u00f6dynt\u00e4\u00e4 muun muassa uusien sy\u00f6p\u00e4hoitojen kehitt\u00e4miseen. Infrapunavalo l\u00e4p\u00e4isee biologista kudosta huomattavasti paremmin kuin muut n\u00e4kyv\u00e4n valon aallonpituudet. Tutkielman tarkoituksena oli rakentaa ensimm\u00e4inen suoraan l\u00e4hi-infrapunavalolla aktivoituva optogeneettinen apoptoosin laukaiseva systeemi. Uusi systeemi perustuu aikaisemmin julkaistuun OptoBAX-systeemiin, jossa apoptoosin aloitus tapahtuu kuljettamalla apoptoottinen BAX-proteiini mitokondrion ulkokalvolle. Uudessa REDeath-systeemiss\u00e4 kuljetus tapahtuu l\u00e4hi-infrapunavalolla aktivoituvan fytokromin avulla. Molempien systeemien toimivuutta testattiin kuvaamalla apoptoosin laukeamista konfokaalimikroskopian avulla. Lis\u00e4ksi apoptoosin laukaisun tehokkuutta yritettiin selvitt\u00e4\u00e4 virtaussytometrian avulla. Verrattuna muihin julkaistuihin systeemeihin apoptoosin laukaisu tapahtui kummallakin systeemill\u00e4 samanlaisella aikav\u00e4lill\u00e4, mutta vaati pienemm\u00e4n valotehon. Apoptoosin laukaisun tehokkuus riippui k\u00e4ytettyjen solulinjojen ominaisuuksista. Lis\u00e4ksi BAX kulkeutui kummallakin systeemill\u00e4 mitokondriolle my\u00f6s pime\u00e4ss\u00e4, mutta se ei aiheuttanut apoptoosin k\u00e4ynnistymist\u00e4. Tutkielman tuloksena syntyi ensimm\u00e4inen suoraan infrapunavalolla aktivoituva optogeneettinen apoptoosin laukaiseva systeemi, REDeath.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetics, a scientific field concentrating on controlling cellular functions by light, has superior spatiotemporal resolution and low invasiveness. One intriguing optogenetic application is the initiation of apoptosis. Controlling apoptosis can answer several questions in basic research and lead to the development of the new cancer treatments. The existing optogenetic apoptosis systems are all activated by blue or green light. Infrared light has higher tissue permeability, which makes it optimal for in vivo studies. The aim of this study was to create the first optogenetic apoptosis system directly activated by near-infrared light. The novel system was built upon the published optogenetic apoptosis system, OptoBAX, which initiates apoptosis by transporting apoptotic BAX protein to mitochondria. In the novel system, REDeath, the transportation was mediated by the near-infrared light activated bacterial phytochrome. The functionality of the original and the novel system was assessed with live confocal microscopy. Also, an attempt to quantify the apoptotic effect was made by flow cytometry. Both systems were capable of inducing apoptosis at a lower illumination power and a similar time scale compared to the other published systems. The cell type specific differences in the effectiveness of the systems were highlighted in the results. Both systems also showed dark activity as colocalization, but without exceeding the apoptotic threshold. Ultimately, the thesis produced the first infrared light activated optogenetic apoptosis system, REDeath.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-03-08T08:38:10Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-03-08T08:38:10Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "126", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "near-infrared", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Light-induced apoptosis : introducing REDeath", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202103081874", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fotodynaaminen hoito", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ohjelmoitunut solukuolema", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "photodynamic therapy", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "programmed cell death", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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