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[{"key": "dc.contributor.advisor", "value": "Pekkala, Satu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Honkanen, Marjaana", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-01-28T06:55:16Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-01-28T06:55:16Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/73862", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Suolistomikrobiston koostumus on yhdistetty ei-alkoholiper\u00e4iseen rasvamaksatautiin (NAFLD). Erityisesti Faecalibacterium prausnitzii -bakteerin runsas m\u00e4\u00e4r\u00e4 suolistossa voi edist\u00e4\u00e4 maksan terveytt\u00e4. Koska t\u00e4t\u00e4 bakteeria ei ole viel\u00e4 hyv\u00e4ksytty hoitomuodoksi ihmisill\u00e4, on t\u00e4rke\u00e4\u00e4 l\u00f6yt\u00e4\u00e4 vaihtoehtoisia keinoja sen luonnollisen m\u00e4\u00e4r\u00e4n lis\u00e4\u00e4miseksi suolistossa. T\u00e4ss\u00e4 tutkimuksessa selvitettiin, voiko ruokaan lis\u00e4tt\u00e4vill\u00e4 ksylo-oligosakkarideilla (XOS) lis\u00e4t\u00e4 F. prausnitzii - bakteerin m\u00e4\u00e4r\u00e4\u00e4 suolistossa ja siten ehk\u00e4ist\u00e4 NAFLD:n kehittymist\u00e4. Lis\u00e4ksi tutkittiin, v\u00e4littyv\u00e4tk\u00f6 XOS:n mahdolliset vaikutukset maksaan rasva- aineenvaihdunnan, insuliinisignaloinnin tai tulehdusvasteen muutosten kautta. Tutkimuksen n\u00e4ytteet saatiin el\u00e4inkokeesta, jossa rasvaisella tai v\u00e4h\u00e4rasvaisella ruokavaliolla olleiden rottien ruokaan joko lis\u00e4ttiin tai ei lis\u00e4tty XOS:ia 12 viikon ajan ennen lopetusta. F. prausnitzii -bakteerin m\u00e4\u00e4r\u00e4\u00e4 suolistossa sek\u00e4 geenien ja signalointiproteiinien ilmentymistasoja maksassa ja epididymaalisessa rasvakudoksessa tutkittiin kvantitatiivisella polymeraasiketjureaktiolla ja vasta-aineiden avulla proteiinin\u00e4ytteist\u00e4. Maksan rasvapitoisuus m\u00e4\u00e4ritettiin Oil Red O \u2013v\u00e4rj\u00e4yksell\u00e4 ja rasvojen uutolla. Rasvainen ruokavalio aiheutti NAFLD:n rotille ja XOS lis\u00e4si F. prausnitzii -bakteerin m\u00e4\u00e4r\u00e4\u00e4 suolistossa molemmilla ruokavalioilla. XOS v\u00e4hensi maksan rasvoittumista rasvaisella ruokavaliolla rasvahappojen hapettumista katalysoivan entsyymin, \u03b2-hydroksiasyyli-KoA-dehydrogenaasin, lis\u00e4\u00e4ntyneest\u00e4 aktiivisuudesta johtuen. XOS kuitenkin lis\u00e4si epididymaalisen rasvakudoksen tulehdusta. Tulosten perusteella XOS on potentiaalinen prebiootti, jota voisi k\u00e4ytt\u00e4\u00e4 maksan rasvoittumisen ehk\u00e4isemisess\u00e4. XOS:n mahdollisia haitallisia sivuvaikutuksia tulisi kuitenkin tutkia tarkemmin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Recently, the gut microbiota composition has been linked to non-alcoholic fatty liver disease (NAFLD). Especially the abundance of Faecalibacterium prausnitzii in the gut has been shown to improve hepatic health. Because this bacterium is not yet accepted for treatment purposes in humans, it is important to find alternative ways to increase its natural abundance in the gut. The aim of this study was to determine whether xylo-oligosaccharide (XOS) supplementation increases the abundance of F. prausnitzii in the gut of rats and thus prevents the development of NAFLD and whether the possible effects of XOS are due to alterations in lipid metabolism, insulin signaling or inflammatory response. The samples used in this thesis were derived from an animal experiment in which rats were fed with high- fat or low-fat diet, supplemented or not with XOS for 12 weeks, and then euthanized. The amount of F. prausnitzii in the gut samples was determined with quantitative polymerase chain reaction (qPCR). Hepatic lipid content was studied with lipid extraction and Oil Red O staining of liver sections. The expression levels of genes and signaling proteins in liver and epididymal adipose tissue were studied by qPCR and western blot, respectively. The results show that high-fat diet caused NAFLD to the rats and XOS increased the abundance of F. prausnitzii on both diets. XOS decreased hepatic lipid content on the high-fat diet due to the increased activity of \u03b2-hydroxyacyl-CoA dehydrogenase, a rate-limiting enzyme of fatty acid oxidation. However, XOS increased epididymal adipose tissue inflammation on the high-fat diet. Based on these results, XOS is a potential prebiotic that could be used to prevent hepatic fat accumulation but the possible adverse side effects of XOS should be studied more closely.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-01-28T06:55:16Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-01-28T06:55:16Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "59", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Faecalibacterium prausnitzii", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "hepatic lipid accumulation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The preventive role of gut microbiota modulating prebiotic xylo-oligosaccharides in non-alcoholic fatty liver disease", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202101281321", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rasvakudokset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rasvamaksa", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistomikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "prebiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "adipose tissues", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fatty liver", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gastrointestinal microbiota", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "prebiotics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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