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[{"key": "dc.contributor.advisor", "value": "Permi, Perttu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Aitio, Helena", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Jartti, Minne", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-12-28T07:01:52Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-12-28T07:01:52Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/73420", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Yksi merkitt\u00e4vimmist\u00e4 ihmisen patogeeneista on Staphylococcus aureus-bakteeri. Se aiheuttaa erityyppisi\u00e4 vakavia infektioita kuten verenmyrkytyst\u00e4 ja syd\u00e4men sis\u00e4kalvon tulehdusta, sek\u00e4 lievempi\u00e4 ihotulehduksia ja ruokamyrkytyst\u00e4. T\u00e4ll\u00e4 hetkell\u00e4 S. aureus-infektioita hoidetaan beetalaktaami-antibiooteilla (esim. penisilliinill\u00e4), mutta antibioottiresistenttien bakteerikantojen kuten MRSA:n yleistymisen vuoksi, on S. aureus-infektioiden hoito eritt\u00e4in hankalaa. Siksi tarve uudentyyppisille stafylokokki-infektioita hoitaville l\u00e4\u00e4keaineille onkin kova. Bakteerien solusein\u00e4m\u00e4\u00e4 pilkkovat entsyymit \u2013 peptidoglykaanihydrolaasit \u2013 ovat er\u00e4s lupaavimmista uudentyyppisist\u00e4 antistafylokokkaalisista l\u00e4\u00e4keaineista. Kuten muillakin gram-positiivisilla bakteereilla, S. aureuksella on paksu bakteerisolua suojaava peptidoglykaani-sein\u00e4m\u00e4. Peptidoglykaanihydrolaasit ovat entsyymej\u00e4, jotka pilkkovat tiettyj\u00e4 kemiallisia sidoksia t\u00e4ss\u00e4 solusein\u00e4m\u00e4ss\u00e4, aiheuttaen siten bakteerisolun tuhoutumisen. Peptidoglykaanihydrolaasien keskeisimm\u00e4t hy\u00f6dyt ovat niiden (1) spesifisyys, sill\u00e4 entsyymit toimivat kohdennetusti pilkkoen juuri tiettyj\u00e4 kemiallisia rakenteita bakteerin peptidoglykaani-solusein\u00e4m\u00e4ss\u00e4, (2) matala myrkyllisyys, (3) entsyymien suhteellisen helppo ja tehokas tuotto, (4) niiden kyky tuhota sek\u00e4 aktiivisia ett\u00e4 inaktiivisia bakteerisoluja ja toimia my\u00f6s biofilmeiss\u00e4, sek\u00e4 lopuksi (5) entsyymien k\u00e4ytt\u00f6 antibioottien kanssa yhdistelm\u00e4hoitona. T\u00e4ss\u00e4 tutkielmassa tarkastellaan erilaisten synteettisten peptidoglykaanifragmenttien pilkkoutumista kahden peptidoglykaani hydrolaasin toimesta. Tutkittavat entsyymit kuuluvat lysostafiini-entsyymiperheeseen. Entsyymien tehokkuutta erilaisten peptidoglykaanifragmenttien katalyysiss\u00e4 tutkittiin k\u00e4ytt\u00e4en liuostilan 1D 1H ydinmagneettista resonanssispektroskopiaa (NMR), joka mahdollistaa hydrolyysireaktioiden reaaliaikaisen seurannan atomiresoluution tasolla. Reaktiotuotteiden rakenteet m\u00e4\u00e4ritettiin 2D NMR -spektroskopialla. Kummallekin tutkittavista entsyymeist\u00e4 l\u00f6ydettiin uusia substraatteja eli bakteerien peptidoglykaanirakenteita, joita ne pilkkovat kohdennetusti. Lis\u00e4ksi peptidoglykaanin entsymaattisesta pilkkoutumisreaktiosta saatiin tarkempaa tietoa, joka mahdollistaa entsyymien modifikaation tulevaisuudessa, ja siten antistafylokokkaalisten l\u00e4\u00e4keaineiden kehityksen.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-12-28T07:01:52Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-12-28T07:01:52Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "92", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "LSS", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "LytU", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "pentaglycine", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "peptidoglycan hydrolases", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Staphylococcus aureus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Determining the substrate specificity of two lysostaphin enzyme family members using NMR spectroscopy", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202012287352", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "entsyymit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibioottiresistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enzymes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiotic resistance", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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