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[{"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Lindberg, Michael", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kantoluoto, Tino Ville Valtteri", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-12-03T09:39:14Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-12-03T09:39:14Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/72949", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Enterovirukset ovat pieni\u00e4 ja vaipattomia positiivisen RNA genomin omaavia viruksia, jotka infektoivat p\u00e4\u00e4asiassa ruoansulatuskanavan epiteelisoluja, mutta voivat muihin kudoksiin levitess\u00e4\u00e4n aiheuttaa erilaisia sairauksia. Echovirus 30 (E30) kuuluu enterovirusten B ryhm\u00e4\u00e4n ja aiheuttaa aivokalvontulehdus-epidemioita ymp\u00e4ri maailmaa. Huolimatta virulenttiudestaan, E30:n perusbiologiasta tiedet\u00e4\u00e4n toistaiseksi hyvin v\u00e4h\u00e4n. T\u00e4ss\u00e4 Pro Gradu -tutkielmassa puhdistettiin E30:t\u00e4 ja tutkittiin sen soluun sis\u00e4\u00e4n menoa ja infektion alkupuolen tapahtumia. Osan enterovirus B -ryhm\u00e4n j\u00e4senten tiedet\u00e4\u00e4n k\u00e4ytt\u00e4v\u00e4n \u201dDecay accelerating factor\u201d (DAF) -proteiinia sek\u00e4 coxsackie- ja adenovirusreseptoria (CAR) soluun tunkeutuessaan. DAF:n on aiemmin osoitettu olevan t\u00e4rke\u00e4 solun tekij\u00e4 E30:n infektiossa. T\u00e4ss\u00e4 tutkielmassa osoitettiin radioaktiivisella reseptorisitoumis- kokeella ett\u00e4 E30 sitoutuu DAF:iin, mutta ei lainkaan CAR:iin. Konfokaalimikroskopiakuvista tehdyt kolokalisaatioanalyysit osoittivat, ett\u00e4 E30 ei k\u00e4yt\u00e4 klatriiniv\u00e4litteist\u00e4 endosytoosireitti\u00e4, mutta siirtyy silti infektion edetess\u00e4 aikaisiin ja my\u00f6h\u00e4isiin endosomeihin. Tyypillisesti enterovirus B ryhm\u00e4n j\u00e4senet eiv\u00e4t esiinny n\u00e4iss\u00e4 solurakenteissa, lukuun ottamatta echovirus 7:\u00e4\u00e4 (E7), joka my\u00f6s on DAF:iin, mutta ei CAR:iin, sitoutuva enterovirus. E30-infektion osoitettiin my\u00f6s etenev\u00e4n nopeasti, sill\u00e4 sen RNA-genomi alkoi replikoituimaan jo 2,5 tunnin j\u00e4lkeen. My\u00f6s is\u00e4nt\u00e4solun solulimakalvostossa osoitettiin tapahtuvan muutoksia jo 2 tunnin j\u00e4lkeen. N\u00e4m\u00e4 tulokset tuovat t\u00e4rke\u00e4\u00e4 tietoa E30-infektion kulusta ja osoittavat samankaltaisuuksia E30:n ja E7:n v\u00e4lill\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Enteroviruses are small, non-enveloped positive sense RNA viruses that primarily target the epithelial cells of the gastrointestinal tract, but are known to cause variety of clinical complications in secondary tissues. Echovirus 30 (E30) is a member of the enterovirus B genus and it causes epidemic bursts of aseptic meningitis all around the world. Despite being a common agent for this neurological disease, not much is known of E30 biology. Here, we have purified E30 and studied its cell entry and early infection using confocal microscopy and receptor binding assay. Some enterovirus B species are known to utilize decay accelerating factor (DAF) and coxsackie and adenovirus receptor (CAR) for cell entry and E30 has also previously been reported to use DAF for its infection. Here, we performed a receptor binding assay using radioactive E30 and showed that E30 indeed binds to DAF, but does not have any affinity towards CAR. Co-localization analyses performed from confocal images with various immunolabeled cell markers showed that E30 is not using the clathrin mediated pathway for its internalization, but still enters the early and late endosomes during infection. Localizing in these endosomal structures is not very common among members of enterovirus B species, apart from echovirus 7 (E7), which is another DAF-but-not-CAR binding virus. E30 showed rapid kinetics for its infection cycle, as genome replication was observed to start already after 2.5 h of infection. Modifications in the host cell endoplasmic reticulum were also observed already at 2 h p.i.. These findings of E30 early infection guide the following research to the right way and makes a connection between E30 and E7 behavior.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-12-03T09:39:14Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-12-03T09:39:14Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "49", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "CAR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "DAF", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "purification", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "endosome", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Cell entry and early infection of Echovirus 30", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202012036904", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enterovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "is\u00e4nt\u00e4solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endosytoosi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "viruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infections", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enteroviruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "host cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endocytosis", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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