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[{"key": "dc.contributor.author", "value": "Per\u00e4kyl\u00e4, Juuli", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-08-11T05:25:09Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-08-11T05:25:09Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/71373", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "S\u00e4\u00e4nn\u00f6llisell\u00e4 liikunnalla on positiivinen vaikutus kokonaisvaltaiseen terveyteen muun muassa parantuneen aerobisen metabolian kautta. Lihassolujen (myotuubien) s\u00e4hk\u00f6pulssistimulaatio (engl. electrical pulse stimulation; EPS) mahdollistaa solutason mekanismien tutkimuksen eristetysti lihassolusupistuksen aikana. Kaikkia solutason adaptaatioita ei ole saatu aiheutettua s\u00e4hk\u00f6pulssistimulaatiolla johdonmukaisesti tai tutkimustulosta on v\u00e4h\u00e4isesti, kuten rasvahappojen hapetuksen ja mitokondrioiden hengityksen osalta. Ty\u00f6n tarkoituksena oli selvitt\u00e4\u00e4 matalafrekvenssisen 24 tunnin s\u00e4hk\u00f6pulssistimulaation vaikutusta (1) rasvahappojen hapetukseen ja sitraattisyntaasin sek\u00e4 \u03b2-hydroksiasyyli-koentsyymi-A dehydrogenaasin aktiivisuuteen, sek\u00e4 (2) liikunnan j\u00e4lkeiseen rutiinihengitykseen ja maksimaaliseen elektroninsiirtoketjun kapasiteettiin k\u00e4ytt\u00e4en C2C12 myotuubeja. Rasvahappojen hapetus mitattiin oleaatin radioaktiivisella hajoamisella ja mitokondrioiden hengityst\u00e4 korkean resoluution respirometrill\u00e4. Rasvahappojen hapetus laski s\u00e4hk\u00f6pulssistimulaation seurauksena, eiv\u00e4tk\u00e4 1 h s\u00e4hk\u00f6pulssistimulaation j\u00e4lkeen mitatut rutiinihengitys ja elektronisiirtoketjun kapasiteetti muuttuneet tilastollisesti merkitt\u00e4v\u00e4sti. K\u00e4ytetty EPS-protokolla saattaakin olla parempi glykolyyttisen, kuin matala-intensiteettisen aerobisen liikunnan mallintamiseen. Tulevaisuuden tutkimuksessa tulisi keskitty\u00e4 stimulaatio -protokollien johdonmukaiseen kehitt\u00e4miseen, jotta pystytt\u00e4isiin kehitt\u00e4m\u00e4\u00e4n luotettavia solumalleja erilaisiin liikuntamuotoihin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Regular exercise promotes overall health by inducing positive adaptations in for instance glucose and fatty acid metabolism and mitochondrial function. However, not all molecular mechanisms behind the health-promoting effects of skeletal muscle contractions are yet completely understood. Electrical pulse stimulation (EPS) has been used as an in vitro exercise mimetic and when applied to cell-culture, it allows research of cellular mechanisms of contracting myotubes in an isolation. There are contradictory findings of EPS-evoked fatty acid oxidation and lack of studies of post-EPS mitochondrial respiration. The aim of this thesis was to examine (1) fatty acid oxidation and activities of citrate synthase (CS) and \u03b2\u2010hydroxyacyl\u2010Coenzyme A dehydrogenase (\u03b2-HAD), and (2) routine respiration and electron transfer system (ETS) capacity after 24-hour low frequency EPS in C2C12 myotubes. Fatty acid oxidation was measured with radioactive labelled oleic acid and liquid scintillation counting and respiration states with high-resolution respirometry. This study revealed that fatty acid oxidation decreased after EPS in C2C12 myotubes independent of glucose availability, while simultaneously CS activity was not consistently changed. \u03b2-HAD measurements were unsuccessful. Additionally, routine respiration or ETS capacity were not altered significantly measured an hour post-EPS. The results suggest that this often used 24-hour EPS myotube contraction protocol may alternatively better mimic glycolytic than low-intensity aerobic exercise. The applied settings of EPS should be determined consistently in the future research to accomplish reliable cell-models for different types of exercise.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-08-11T05:25:09Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-08-11T05:25:09Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "47", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "electrical pulse stimulation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fatty acid oxidation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mitochondrial respiration", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Modelling exercise-metabolism with electrical pulse stimulated C2C12 myotubes", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202008115515", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "liikunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rasvahapot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihassolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "physical training", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fatty acids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "muscle cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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