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[{"key": "dc.contributor.advisor", "value": "Lindstr\u00f6m, Leena", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Vehni\u00e4inen, Eeva-Riikka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kivi\u00f6, Ronja", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-07-30T07:49:59Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-07-30T07:49:59Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/71290", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Torjunta-aineiden k\u00e4ytt\u00f6 on lis\u00e4nnyt niille vastustuskykyisten lajien m\u00e4\u00e4r\u00e4\u00e4 ymp\u00e4ri\nmaailmaa. Torjunta-ainevastustuskykyyn on yhdistetty mm. ATP:t\u00e4 sitovat\nkasettikuljettimet (ABC), jotka esimerkiksi poistavat myrkyllisi\u00e4 yhdisteit\u00e4 solusta.\nKoloradonkuoriainen, Leptinotarsa decemlineata, on laajalle levinnyt haitallinen\ntuholainen, joka k\u00e4ytt\u00e4\u00e4 ravintonaan mm. perunaa, ja sen torjuminen on vaikeaa,\nsill\u00e4 se on vastustuskykyinen v\u00e4hint\u00e4\u00e4n 56 torjunta-aineelle. Tavoitteenani oli\ntunnistaa ABC-geenej\u00e4 koloradonkuoriaisen perim\u00e4st\u00e4, sek\u00e4 selvitt\u00e4\u00e4, ovatko ne\nyhteydess\u00e4 sen torjunta-ainevastustuskykyyn. Tutkin 1) vaikuttavatko\natsinfossimetyyli (AZ; organofosfaatti) ja karbaryyli (CAR; karbamaatti) ABCgeenin\nilmentymiseen, sek\u00e4 2) eroaako niiden ilmentyminen torjunta-aineille\nvastustuskykyisen (Vermont, U.S.A) ja alttiimman (Belchow, Puola) populaation\nv\u00e4lill\u00e4. 18 yksil\u00f6\u00e4 (yht. 36) kummastakin populaatiosta altistettiin joko torjuntaaineelle\ntai asetonille (kontrolli). Eristin RNA:n, valmistin komplementaarista\nDNA:ta ja analysoin kuuden ABC-geenin (ABCBmember6, ABCBmember7,\nABCC13, ABCG1041A, ABCH278B, ABCH278C) ilmentymistasoja qPCRmenetelm\u00e4ll\u00e4.\nSek\u00e4 AZ ett\u00e4 CAR voimistivat kaikkien ABC-geenien ilmentymist\u00e4.\nAZ-k\u00e4sittelyn j\u00e4lkeen ABCBmember6-geeni ilmentyi vastustuskykyisess\u00e4\npopulaatiossa enemm\u00e4n, kun taas ABCC13 ilmentyminen oli suurempaa alttiissa\npopulaatiossa. Ilmentymisess\u00e4 ei havaittu eroja populaatioiden v\u00e4lill\u00e4 CAR- tai\nkontrollik\u00e4sittelyn j\u00e4lkeen. Kaiken kaikkiaan ABC-geenit vaikuttavat olevan\nt\u00e4rkeit\u00e4 kuoriaisen suojaamisessa torjunta-aineilta. Etenkin ABCBmember6 on\nvahva ehdokas organofosfaattivastustuskyvyn osatekij\u00e4ksi koloradonkuoriaisella.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Insecticide resistance is a global issue caused by ever-increasing use of insecticides.\nOne gene-family linked to the resistance is the family of ATP-binding cassette (ABC)\ntransporters which e.g. excrete toxins from the cytoplasm. The Colorado potato\nbeetle (CPB), Leptinotarsa decemlineata, is a widespread pest species. CPB causes\nmassive losses to for instance potato crops and is hard to control due to its high\nresistance to insecticides: the beetle is known to be resistant to 56 insecticides. My\naim was to identify ABC genes in the genome of CPB and find out if they are linked\nto insecticide resistance. I wanted to know 1) if azinphos-methyl (AZ;\norganophosphate) and carbaryl (CAR; carbamate) influence the expression of ABC\ngenes in CPB and 2) whether the expressions differ between resistant (Vermont,\nU.S.A.) and more susceptible (Belchow, Poland) populations. 18 individuals (total\n36) from each population were treated with either an insecticide or acetone (control).\nI extracted their RNA, synthesized cDNA, and performed qPCR with primers of six\ndifferent ABC genes (ABCBmember6, ABCBmember7, ABCC13, ABCG1041A,\nABCH278B, ABCH278C). Exposure to both AZ and CAR elevated the expression\nrates of all ABC genes across the populations. After AZ treatment, ABCBmember6\nwas more upregulated in the resistant population, whereas ABCC13 was more\nupregulated in the sensitive one. The gene expressions did not differ between the\npopulations in either CAR or control groups. My study supports the importance of\nABC genes in protecting CPB against these insecticides. Especially ABCBmember6\nis a probable candidate for being a part of organophosphate resistance in the beetle.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-07-30T07:49:59Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-07-30T07:49:59Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "44", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "insecticide resistance", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "azinphos-methyl", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "carbaryl", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "organophosphate", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "qPCR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The link between ABC genes and insecticide resistance in Leptinotarsa decemlineata", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202007305438", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ekologia ja evoluutiobiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ecology and evolutionary biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4011", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "karbamaatit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniekspressio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insektisidit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "torjunta-aineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuhohy\u00f6nteiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "torjunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "koloradonkuoriainen", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuhoel\u00e4imet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aakkoset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genomiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "carbamates", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gene expression", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insecticides", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pesticides", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insect pests", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "control (prevention)", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Colorado potato beetle", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pests", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ABC", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genomics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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