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[{"key": "dc.contributor.advisor", "value": "Pentik\u00e4inen, Ulla", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Roivas, Pekka", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-07-06T05:59:08Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-07-06T05:59:08Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/71065", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Fosfataasit ovat t\u00e4rkeit\u00e4 solun sis\u00e4ll\u00e4 tapahtuvan s\u00e4\u00e4telyn kannalta.\nProteiinifosfataasi 2A (PP2A) on yksi n\u00e4ist\u00e4 s\u00e4\u00e4telij\u00f6ist\u00e4 ja sen normaali toiminta\nliittyy solusyklin aktivointiin. Kun PP2A:n normaalia toimintaa h\u00e4irit\u00e4\u00e4n, voi sen\nseurauksena ilmet\u00e4 erilaisia sairauksia, kuten sy\u00f6p\u00e4\u00e4. PP2A:n toimintaa est\u00e4vien\ninhibiittoriproteiinien on huomattu olevan mahdollinen syy t\u00e4h\u00e4n h\u00e4iri\u00f6\u00f6n.\nInhibiittoriproteiinien, kuten fosfoproteiini/\uf061-endosulfiini perheen proteiinien ja\nCancerous Inhibitor of PP2A (CIP2A) on huomattu est\u00e4v\u00e4n PP2A:ta, mutta niiden\nsitoutumismekanismeista PP2A:n tiedet\u00e4\u00e4n v\u00e4h\u00e4n. PP2A on heterotrimeerinen\nproteiini ja se koostuu tuki-, s\u00e4\u00e4telij\u00e4- ja katalyyttisest\u00e4 alayksik\u00f6st\u00e4. PP2A:n\ns\u00e4\u00e4telyalayksikk\u00f6 B:n sis\u00e4ll\u00e4 on tavattu ainakin 23 erilaista isoformia. T\u00e4ss\u00e4\ntutkimuksessa inhibiittoriproteiinien sitoutumista mitataan kolmeen eri PP2A B\nalayksik\u00f6\u00f6n B56\uf067, B56\uf064 ja B56\u03b5. Fosfoproteiini/\uf061-endosulfiini perheen\ninhibiittoriproteiinien sitoutumisvoimakuutta tutkitaan kahden erilaisen\nfosforylaatiota matkivan mutaation avulla. Ensin B alayksik\u00f6n proteiinit tuotettiin\nja puhdistettiin, ja niiden proteiiniproteiini vuorovaikutuksia tutkittiin microscale\nthermophoresis menetelm\u00e4ll\u00e4. Tulokset osoittivat, ett\u00e4 fosfoproteiini/\uf061-\nendosulfiini perheen villityyppi proteiinit sitoutuivat kaikkiin tutkittuihin B56\nalayksik\u00f6ihin. CIP2A:n sitoutuminen oli vaihtelevaa. Fosforylaatiota matkivat\nmutaatiot lis\u00e4siv\u00e4t sitoutumisvoimakkuutta cAMP-regulated phosphoprotein 19\nkDa (ARPP19) ja cAMP-regulated phosphoprotein 16 kDa (ARPP16) kohdalla,\nmutta ei alfa-endosulfiinin (ENSA) kanssa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Phosphatases are important factors in cellular regulation. Protein phosphatase 2A (PP2A) is one of these regulators and its functions are related in activation of cell cycle. When the normal functions of PP2A are disturbed it can lead to various diseases, including cancer. This disturbance can be caused by various protein inhibitors. Inhibitor proteins from cAMP regulated phosphoprotein/a-endosulfin family and Cancerous Inhibitor of PP2A (CIP2A) has already been confirmed as PP2A inhibitors, but their inhibition mechanisms are mostly unknown. PP2A is a heterotrimeric protein that consists of scaffolding, regulatory and catalytic subunit. At least 23 different isoforms have been discovered between its regulatory B subunits. In this study, the binding of these inhibitor proteins is measured with three different PP2A B subunit isoforms B56g, B56d and B56e. To activate the binding capabilities of cAMP regulated phosphoprotein/a-endosulfin family inhibitors, two different phosphomimicking mutants for each inhibitor was also tested. These B56 isoforms were first expressed and purified, and then their protein-protein interaction with these inhibitor proteins was studied using microscale thermophoresis method. The results showed that wild type cAMP regulated phosphoprotein/a-endosulfin family inhibitors bound to all these subunits. Binding of CIP2A domains was varying. cAMP-regulated phosphoprotein 19 kDa (ARPP19) and cAMP-regulated phosphoprotein 16 kDa (ARPP16) bearing phosphomimicking mutations binds to B56 isoforms, whereas alpha-endosulfine (ENSA) phosphomimicking mutation did not show binding.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2020-07-06T05:59:08Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-07-06T05:59:08Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "48", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "ARPP16", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "ARPP19", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "CIP2A", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "ENSA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Phosphatase", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Phosphomimic", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Interactions of PP2A inhibitor proteins with PP2A regulatory subunits", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202007065238", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "inhibiittorit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aminohapot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fosforylaatio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "vuorovaikutus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "inhibitors", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "amino acids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "phosphorylation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "interaction", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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