Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria

Polysykliset aromaattiset hiilivedyt (PAH) ovat kaikkialle maailmaan levinnyt joukko usein karsinogeenisiä ja hitaasti hajoavia maaperän saasteita. Fytoremediaatio on maaperänpuhdistusmetodi, jossa kasveja käytetään tukemaan maaperän saasteita hajottavia mikrobeja. Tässä pro gradu-tutkielmassa tutki...

Full description

Bibliographic Details
Main Author: Kovakoski, Harri
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2020
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/69808
_version_ 1826225778305531904
author Kovakoski, Harri
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Kovakoski, Harri Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Kovakoski, Harri Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Kovakoski, Harri
datasource_str_mv jyx
description Polysykliset aromaattiset hiilivedyt (PAH) ovat kaikkialle maailmaan levinnyt joukko usein karsinogeenisiä ja hitaasti hajoavia maaperän saasteita. Fytoremediaatio on maaperänpuhdistusmetodi, jossa kasveja käytetään tukemaan maaperän saasteita hajottavia mikrobeja. Tässä pro gradu-tutkielmassa tutkin maaperän pH:n ja glukoosilisän vaikutusta fenantreenin (PHE) hajoamiseen maanäytteissä, jotka oli kerätty Suomen Luonnonvarakeskuksen (LUKE) hallinnoimalta fytoremediaatio-alueelta Somerharjulta, Luumäeltä, jossa kasvoi eri haapalajeja. Kunnostettava alue on puretun kreosoottikyllästämön saastuttama ja Suomen Luonnonvarakeskus (LUKE) on aloittanut fytoremediaation vuonna 2013. Radioleimatun PHE:n hajoamista tutkittiin MicrorespTM-laitteella kaksi viikkoa kestävässä kokeessa. Maanäytteiden metagenomi sekvensointiin seuraavan sukupolven geenisekvensointimenetelmillä (NGS) näytteiden mikrobien diversiteetin selvittämiseksi. Sekä metsähaavat että hybridihaavat lisäsivät PHE hajotusta tilastollisesti merkittävästi kontrolleihin verrattuna, mutta ei toisiinsa verrattuna. pH:lla ja glukoosilla ei ollut tilastollisesti merkittävää vaikutusta hajotukseen. Mikrobien paikallinen diversiteetti oli korkeimmillaan kontrolleissa, mutta puita sisältävissä näytteissä oli enemmän näytteiden välistä variointia. Tulokset tarjoavat mahdollisuuksia avainlaji P. tremulan ja kohdennettujen bakteerilajien käyttöön puhdistuksessa. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous, often carcinogenic and persistent soil pollutants that disperse over long distances. In phytoremediation plants are used to support soil microbiota capable of degrading pollutants. I studied the effects of soil pH and glucose supplement on the degradation of phenanthrene (PHE) in soil samples collected from a phytoremediation site consisting of a plantation of different aspen species. The site at Somerharju, Luumäki, was originally contaminated with PAH-compounds during creosote impregnation, and the phytoremediation experiment was started by Finnish Natural Resources Centre (LUKE) in 2013. The soil respiration of radiolabelled PHE was studied with the MicrorespTM device in a two-week incubation experiment. Microbial content of soil samples’ was sequenced using next generation sequencing (NGS) of the 16S rRNA gene to explore the differences in the microbiota diversity of the rhizosphere. Both aspen species increased PHE degradation of the rhizosphere soil samples when compared to controls, but no difference was observed between the plots planted with European aspen or hybrid aspen. Soil pH and the addition of glucose did not significantly increase degradation of PHE. Local diversity was largest in soil samples collected from plots without trees, while the plots with trees had more between-samples variation. The results suggest possibilities for the use of the key species P. tremula and targeted bacteria strains in remediation.
first_indexed 2024-09-11T08:48:56Z
format Pro gradu
free_online_boolean 1
fullrecord [{"key": "dc.contributor.advisor", "value": "Tiirola, Marja", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Hopkins, David", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pulkkinen, Pertti", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kovakoski, Harri", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-06-09T11:48:44Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-06-09T11:48:44Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/69808", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Polysykliset aromaattiset hiilivedyt (PAH) ovat kaikkialle maailmaan levinnyt joukko usein karsinogeenisi\u00e4 ja hitaasti hajoavia maaper\u00e4n saasteita. Fytoremediaatio on maaper\u00e4npuhdistusmetodi, jossa kasveja k\u00e4ytet\u00e4\u00e4n tukemaan maaper\u00e4n saasteita hajottavia mikrobeja. T\u00e4ss\u00e4 pro gradu-tutkielmassa tutkin maaper\u00e4n pH:n ja glukoosilis\u00e4n vaikutusta fenantreenin (PHE) hajoamiseen maan\u00e4ytteiss\u00e4, jotka oli ker\u00e4tty Suomen Luonnonvarakeskuksen (LUKE) hallinnoimalta fytoremediaatio-alueelta Somerharjulta, Luum\u00e4elt\u00e4, jossa kasvoi eri haapalajeja. Kunnostettava alue on puretun kreosoottikyll\u00e4st\u00e4m\u00f6n saastuttama ja Suomen Luonnonvarakeskus (LUKE) on aloittanut fytoremediaation vuonna 2013. Radioleimatun PHE:n hajoamista tutkittiin MicrorespTM-laitteella kaksi viikkoa kest\u00e4v\u00e4ss\u00e4 kokeessa. Maan\u00e4ytteiden metagenomi sekvensointiin seuraavan sukupolven geenisekvensointimenetelmill\u00e4 (NGS) n\u00e4ytteiden mikrobien diversiteetin selvitt\u00e4miseksi. Sek\u00e4 mets\u00e4haavat ett\u00e4 hybridihaavat lis\u00e4siv\u00e4t PHE hajotusta tilastollisesti merkitt\u00e4v\u00e4sti kontrolleihin verrattuna, mutta ei toisiinsa verrattuna. pH:lla ja glukoosilla ei ollut tilastollisesti merkitt\u00e4v\u00e4\u00e4 vaikutusta hajotukseen. Mikrobien paikallinen diversiteetti oli korkeimmillaan kontrolleissa, mutta puita sis\u00e4lt\u00e4viss\u00e4 n\u00e4ytteiss\u00e4 oli enemm\u00e4n n\u00e4ytteiden v\u00e4list\u00e4 variointia. Tulokset tarjoavat mahdollisuuksia avainlaji P. tremulan ja kohdennettujen bakteerilajien k\u00e4ytt\u00f6\u00f6n puhdistuksessa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous, often carcinogenic and persistent soil pollutants that disperse over long distances. In phytoremediation plants are used to support soil microbiota capable of degrading pollutants. I studied the effects of soil pH and glucose supplement on the degradation of phenanthrene (PHE) in soil samples collected from a phytoremediation site consisting of a plantation of different aspen species. The site at Somerharju, Luum\u00e4ki, was originally contaminated with PAH-compounds during creosote impregnation, and the phytoremediation experiment was started by Finnish Natural Resources Centre (LUKE) in 2013. The soil respiration of radiolabelled PHE was studied with the MicrorespTM device in a two-week incubation experiment. Microbial content of soil samples\u2019 was sequenced using next generation sequencing (NGS) of the 16S rRNA gene to explore the differences in the microbiota diversity of the rhizosphere. Both aspen species increased PHE degradation of the rhizosphere soil samples when compared to controls, but no difference was observed between the plots planted with European aspen or hybrid aspen. Soil pH and the addition of glucose did not significantly increase degradation of PHE. Local diversity was largest in soil samples collected from plots without trees, while the plots with trees had more between-samples variation. The results suggest possibilities for the use of the key species P. tremula and targeted bacteria strains in remediation.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-06-09T11:48:44Z\r\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-06-09T11:48:44Z (GMT). No. of bitstreams: 0\r\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "63", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "16SrRNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Microresp", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "radiomethod", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202006094051", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ymp\u00e4rist\u00f6tiede ja -teknologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Environmental science and technology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4015", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glukoosi", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "maaper\u00e4", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "haapa", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aromaattiset hiilivedyt", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hybridihaapa", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fytoremediaatio", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "juuristo", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "PAH-yhdisteet", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glucose", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "soil", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aspen", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aromatic hydrocarbons", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hybrid aspen", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "phytoremediation", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "root system", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "PAH compounds", "language": "", "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
id jyx.123456789_69808
language eng
last_indexed 2025-02-18T10:56:12Z
main_date 2020-01-01T00:00:00Z
main_date_str 2020
online_boolean 1
online_urls_str_mv {"url":"https:\/\/jyx.jyu.fi\/bitstreams\/0d5e13ac-b88a-4aeb-a261-7f9e0e8c16ae\/download","text":"URN_NBN_fi_jyu-202006094051.pdf","source":"jyx","mediaType":"application\/pdf"}
publishDate 2020
record_format qdc
source_str_mv jyx
spellingShingle Kovakoski, Harri Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria 16SrRNA Microresp radiomethod Ympäristötiede ja -teknologia Environmental science and technology 4015 glukoosi maaperä haapa aromaattiset hiilivedyt hybridihaapa fytoremediaatio juuristo PAH-yhdisteet glucose soil aspen aromatic hydrocarbons hybrid aspen phytoremediation root system PAH compounds
title Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_full Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_fullStr Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_full_unstemmed Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_short Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_sort phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
title_txtP Phenanthrene biodegradation potential and diversity of aspen rhizosphere bacteria
topic 16SrRNA Microresp radiomethod Ympäristötiede ja -teknologia Environmental science and technology 4015 glukoosi maaperä haapa aromaattiset hiilivedyt hybridihaapa fytoremediaatio juuristo PAH-yhdisteet glucose soil aspen aromatic hydrocarbons hybrid aspen phytoremediation root system PAH compounds
topic_facet 16SrRNA 4015 Environmental science and technology Microresp PAH compounds PAH-yhdisteet Ympäristötiede ja -teknologia aromaattiset hiilivedyt aromatic hydrocarbons aspen fytoremediaatio glucose glukoosi haapa hybrid aspen hybridihaapa juuristo maaperä phytoremediation radiomethod root system soil
url https://jyx.jyu.fi/handle/123456789/69808 http://www.urn.fi/URN:NBN:fi:jyu-202006094051
work_keys_str_mv AT kovakoskiharri phenanthrenebiodegradationpotentialanddiversityofaspenrhizospherebacteria