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[{"key": "dc.contributor.advisor", "value": "Ollila, Saara", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lemmetyinen, Toni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-05-11T06:10:50Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-05-11T06:10:50Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/68909", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Enteroendokriinisolu (EE-solu) on yksi suoliston normaalia toimintaa yll\u00e4pit\u00e4v\u00e4 solutyyppi, jonka on todettu v\u00e4litt\u00e4v\u00e4n hermostosta tulevia viestej\u00e4. Aikaisemmassa tutkimuksessa EE-solujen on todettu muuntuvan takaisin kantasoluiksi ja edesauttaneen suoliston uusiutumista, mik\u00e4li alkuper\u00e4iset kantasolut ovat tuhoutuneet. N\u00e4iden EE-solujen on havaittu ilment\u00e4v\u00e4n RET reseptoria. RET on tyrosiinikinaasireseptori neurotrofisille kasvutekij\u00f6ille, joita eritet\u00e4\u00e4n alueella, jossa kantasoluja sijaitsee. On mahdollista, ett\u00e4 neurotrofiset kasvutekij\u00e4t vaikuttavat suoliston kantasoluihin ja niist\u00e4 polveutuvien EE-solujen tasapainon s\u00e4\u00e4telyyn RET reseptorin kautta. T\u00e4ten RET signaloinnilla voi olla vaikutusta suoliston uusiutumiseen ja sen kehittymiseen. T\u00e4ss\u00e4 tutkimuksessa haluttiin todentaa RET-solujen esiintyminen suoliston epiteeliss\u00e4 ja selvitt\u00e4\u00e4, pystyv\u00e4tk\u00f6 RET-solut toimimaan kantasoluina organoidikasvatuksessa. Lis\u00e4ksi haluttiin tutkia, onko neurotrofisilla kasvutekij\u00f6ill\u00e4 vaikutusta RET-solujen erilaistumiseen tai kykyyn toimia kantasoluina. Tutkimuksessa k\u00e4ytettiin hiirimallia, jossa Ret geeniin oli liitetty fluoresoiva merkkigeeni. T\u00e4m\u00e4n avulla RET-soluja pystyttiin leimaamaan yhdess\u00e4 EE-soluille tyypillisten proteiinien kanssa sek\u00e4 erist\u00e4m\u00e4\u00e4n yksitt\u00e4isi\u00e4 soluja virtaussytometrialla. Yksitt\u00e4isi\u00e4 RET-soluja eristettiin organoidikasvatukseen sek\u00e4 mitattiin geenien ilmentymist\u00e4 kvantitatiivisen polymeraasiketjureaktion avulla. RET reseptoria ilment\u00e4vi\u00e4 soluja l\u00f6ytyi sek\u00e4 suolikuopakkeesta ett\u00e4 nukkalis\u00e4kkeest\u00e4 ja solut ilmensiv\u00e4t EE-soluille tyypillisi\u00e4 proteiineja. Tutkimus ei pystynyt selvitt\u00e4m\u00e4\u00e4n neurotrofisten kasvutekij\u00f6iden vaikutusta yksitt\u00e4isille RET-soluille, sill\u00e4 RET-solujen kyky toimia kantasoluina ja muodostaa organoideja sai ristiriitaisia tuloksia. Suolikuopakkeista kasvatetuissa organoideissa neurotrofiset kasvutekij\u00e4t kasvattivat EE-solujen m\u00e4\u00e4r\u00e4\u00e4 ja mRNA tasoja. T\u00e4ss\u00e4 tutkimuksessa pystyttiin todentamaan RET-solut suoliston epiteeliss\u00e4 EE-soluiksi ja havaittiin neurotrofisilla kasvutekij\u00f6ill\u00e4 olevan vaikutusta EE-solujen erikoistumiseen organoidikasvatuksessa. Yksitt\u00e4isten RET-solujen kyky toimia kantasoluina ja neurotrofisten kasvutekij\u00f6iden vaikutus suoliston kantasolujen s\u00e4\u00e4telyyn vaatii viel\u00e4 lis\u00e4tutkimusta.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "One of the cell types in small intestine epithelium are hormone secreting enteroendocrine cells (EECs), which are known to interact with neuronal signals. Previously, a subset of EECs has been shown to act as reserve stem cells when homeostatic stem cells are destroyed. We found that these specific EECs express mRNA of the tyrosine kinase receptor RET. RET is the receptor for neurotrophic factors, which are secreted by fibroblasts adjacent to intestinal stem cells. We hypothesize that the stemness-differentiation balance of the EECs is regulated by neurotrophic factors via RET receptor and thus RET signaling has a role in regeneration and maturation of the intestinal epithelium. The aim of this study was to characterize RET-expressing cells in the intestinal epithelium and to test whether single RET-expressing cells act as stem cells by growing them ex vivo in organoid culture. In addition, we wanted to address whether neurotrophic factors affect the stemness or differentiation of RET+ cells. To address these aims, a specific mouse model was used where a fluorescent marker gene was inserted into a Ret gene locus enabling the co-staining of RET-expressing cells with EEC markers and isolation of single cells with fluorescence activated cell sorter followed by gene expression analysis by quantitative PCR. RET-expressing cells were found in crypts and villi and they expressed EEC markers. Isolated RET+ cells could act as stem cells in organoid culture, but the result was not repetitive giving inconclusive results. The effect of neurotrophic factors on single RET+ cells could not be achieved, however, neurotrophic factors increased the expression levels of EEC markers and the amount of EEC cells in ex vivo crypt culture. In conclusion, RET is expressed in EECs and neurotrophic factors increase EEC marker expression suggesting a role in EEC differentiation. The study could not conclude whether RET+ cells can act as reserved stem cells or whether neurotrophic factors regulate intestinal epithelium stem cells.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2020-05-11T06:10:50Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-05-11T06:10:50Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "48", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "enteroendocrine cells", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "glial cell line-derived neurotrophic factor", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "organoid", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tyrosine kinase", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Characterization of RET-expressing cells in the intestinal crypt", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202005113115", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kantasolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ohutsuoli", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "stem cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "small intestine", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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