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[{"key": "dc.contributor.advisor", "value": "Turkki, Vesa", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Sundberg, Lotta-Riina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kekkonen, Nanne", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-03-10T09:48:30Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-03-10T09:48:30Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/68113", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Geeniterapiassa sairautta hoidetaan geenin siirrolla, usein viem\u00e4ll\u00e4 siirtogeeni soluun kuljettimen eli vektorin avulla. Yleisimpi\u00e4 k\u00e4ytettyj\u00e4 vektoreita ovat virukset johtuen niiden luontaisesta kyvyst\u00e4 siirt\u00e4\u00e4 perim\u00e4ns\u00e4 solujen monistettavaksi. Virusvektoreiden valmistuksessa lopputuotteen ominaisuuksiin voidaan vaikuttaa genomin muokkaamisella, mutta my\u00f6s esimerkiksi muokkaamalla tuottosolun toimintaa tai tuottamalla tuottosoluissa viruksen ulkopuolisia proteiineja. Adenoviruksen proteiini IX (pIX) sijaitsee viruksen kapsidissa ja sen ensisijaisena teht\u00e4v\u00e4n\u00e4 on stabiloida kapsidin rakennetta. On kuitenkin osoitettu, ett\u00e4 t\u00e4ll\u00e4 proteiinilla on muitakin rooleja adenovirusinfektiossa. T\u00e4ss\u00e4 tutkimuksessa oli tarkoitus selvitt\u00e4\u00e4 nostaako pIX:n yliekspressio villityypin adenoviruksen ja kolmen eri genomin omaavan adenovirusvektorin tuottavuutta tuottosoluissa. Vektoreita tuotettiin sek\u00e4 adherentti- ett\u00e4 suspensiosoluissa, pIX:n yliekspression kanssa tai ilman. Vektorit puhdistettiin ultrasentrifugoinnilla sek\u00e4 dialyysilla ja niiden tiitterit m\u00e4\u00e4ritettiin ddPCR:n avulla. Lis\u00e4ksi tutkielmassa pyrittiin havaitsemaan pIX:n sijoittumista tuottosoluissa leimaamalla pIX:ll\u00e4 transfektoituja soluja. pIX:n yliekspression ei havaittu nostavan virusvektoreiden tuottavuutta adherenttisoluissa, kun taas suspensiosolujen kohdalla tuottavuus nousi osalla. Suspensiossa onnistuttiin my\u00f6s tuottamaan ei-pIX:\u00e4 sis\u00e4lt\u00e4v\u00e4\u00e4 adenovirusvektoria samalla tehokkuudella kuin pIX:\u00e4 sis\u00e4lt\u00e4v\u00e4\u00e4 virusvektoria. T\u00e4m\u00e4 oli iso saavutus, sill\u00e4 suspensiotuottoihin siirtyminen mahdollistaisi tulevaisuudessa kyseisen vektorin tuoton skaalauksen kaupallisiin suuren mittakaavan tuottoihin. Virusvektoreiden valmistusprosessien optimoinnilla voidaan merkitt\u00e4v\u00e4sti v\u00e4hent\u00e4\u00e4 tuotannon kustannuksia ja n\u00e4in ollen eri virusvektorien tuottavuuden biologian tutkiminen on t\u00e4rke\u00e4\u00e4 nyt ja tulevaisuudessa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "In gene therapy the disease is treated by gene delivery often by transporting a transgene into cells with a vector. The most used vectors are viruses that have the natural ability to transfer their genes into cells. In the production of viral vectors, the features of the final product can be affected by manipulating the genome but also by modifying the function of the producer cell or by producing proteins in the producer cells. The adenovirus protein IX (pIX) is located in the virus capsid and its primary function is to stabilize the capsid. However, studies have also suggested that this minor protein embraces other roles in the adenovirus life cycle. In this study the aim was to find out whether overexpression of pIX increases the production of one wild type adenovirus and three different adenoviral vectors. These vectors were produced in adherent and suspension cells, with and without the overexpression of pIX. The viral vectors were purified by ultracentrifugation and dialysis and their titers were determined by ddPCR. In addition, the localization of pIX in the producer cells was examined by staining the pIXtransfected cells. The results showed that the overexpression of pIX didn\u2019t increase the productivity of viral vectors in adherent cells, whereas in suspension cells the productivity increased with some vectors. Also, non-pIX-containing adenoviral vector was produced in suspension at the same efficiency as a pIX-containing vector. This was a big achievement because the transition to suspension manufacturing would enable to scale up the production of this viral vector and meet the large demand that commercialization requires. Optimizing viral vector manufacturing processes can significantly reduce production costs and therefore studying the productivity biology of different viral vectors is important now and in the future.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2020-03-10T09:48:30Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-03-10T09:48:30Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "72", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Proteiini IX:n yliekspression vaikutus adenovirusvektoreiden tuottoon", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202003102352", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.collaborator", "value": "business", "language": "", "element": "contractresearch", "qualifier": "collaborator", "schema": "yvv"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "business", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "adenovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniterapia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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