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[{"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lommi, Sara", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-01-15T08:03:28Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-01-15T08:03:28Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/67312", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Tyypin 1 diabetes on krooninen autoimmuunisairaus, jossa kehon glukoositasapaino on h\u00e4iriintynyt. Tautia sairastavan potilaan haiman beta-solut ovat tuhoutuneet, mink\u00e4 takia insuliinia ei muodostu tarpeeksi kehon sokeriaineenvaihdunnan yll\u00e4pit\u00e4miseen. Geenien lis\u00e4ksi jotkin ymp\u00e4rist\u00f6tekij\u00e4t, kuten virukset, vaikuttavat mahdollisesti taudin puhkeamiseen. P\u00e4\u00e4ep\u00e4iltyn\u00e4 ovat enterovirukset, pienet RNA-virukset, jotka aiheuttavat flunssaa, mutta my\u00f6s vakavia akuutteja ja kroonisia tulehduksia. T\u00e4m\u00e4n tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4, voidaanko enterovirusinfektiota havaita tyypin 1 diabeteshaimakudoksessa ja erityisesti insuliinia tuottavissa beta-soluissa. Lis\u00e4ksi p\u00e4\u00e4m\u00e4\u00e4r\u00e4n\u00e4 oli erotella haiman eri solutyypit markkeriproteiinien avulla. Haimakudoksen tarkastelu tapahtui Tokyuasun immuno-elektronimikroskopiatekniikan avulla. Ensin puoliohuista leikkeist\u00e4 leimattiin fluoresenssitekniikalla viruskapsidi ja RNA. T\u00e4m\u00e4n mukaan valittiin positiivisia n\u00e4ytteit\u00e4 ohutleikkeisiin ja proteiini A-kultaleimauksiin, jotka kuvattiin elektronimikroskoopilla. Kartoittaessa eri solutyyppej\u00e4, huomattiin beta-solujen harvalukuinen esiintyminen diabeteshaimassa. Glukagonia tuottavia alfa-soluja sen sijaan l\u00f6ytyi reilusti ja muiden solutyyppien edustajia vain yksitt\u00e4isi\u00e4, jos lainkaan. Vain muutama virusinfektiolle heikosti positiivinen beta-solu l\u00f6ytyi diabetes- ja autoantibody-positiivisesta n\u00e4ytteest\u00e4. T\u00e4m\u00e4 tekniikka soveltuu siis heikkojen signaalien havaitsemiseen, mutta on hyvin ty\u00f6l\u00e4s etsitt\u00e4ess\u00e4 harvassa olevia positiivisia n\u00e4ytekohtia.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Type 1 diabetes is a chronic autoimmune disease in which the glucose homeostasis is disrupted. The patient\u2019s pancreatic beta cells are destructed and therefore the insulin production is decreased. Genetic background has been shown to be associated with the development of Type 1 diabetes but also some environmental factors such as viruses seem to have a role. The prime viral suspects are human enteroviruses: the small RNA viruses that cause mild diseases such as common cold but also serious acute and chronic infections. This thesis was focused on detecting enteroviruses from Type 1 diabetic pancreas tissue and especially from insulin producing beta cells. In addition, the different cell types of pancreas were confirmed with the marker proteins. The pancreas tissue was examined for virus infection with Tokyuasu\u2019s immuno-electron microscopy technique. First, semi-thin sections were labeled for virus capsid and RNA by immunofluorescence. Then infected areas were chosen for thin sectioning, protein A gold labeling and electron microscope imaging. From the diabetic pancreas tissue, only a few beta cells were found. Instead, the amount of glucagon producing alfa cells was generous. The other pancreatic cell types were found poorly or not found at all in both diabetic and control pancreas tissues. Majority of the cells showed no indication of virus infection. Only a few beta cells from autoantibody positive and diabetic tissues gave low but statistically significant signs of infection suggesting that this technique may be used to detect low signal but is tedious method for finding small infected areas.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2020-01-15T08:03:28Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-01-15T08:03:28Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "60", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "beta-solu", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Detecting Enterovirus Infection in Type 1 Diabetic Pancreas Tissue Using Immuno-Electron Microscopy", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202001151261", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "nuoruustyypin diabetes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "diabetes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enterovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insuliini", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "autovasta-aineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infections", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "juvenile diabetes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "diabetes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "viruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enteroviruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insulin", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "autoantibodies", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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