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[{"key": "dc.contributor.advisor", "value": "Mattila, Sari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ojala, Ville", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Tuononen, Timo", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2019-12-18T06:49:45Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2019-12-18T06:49:45Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/66894", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibioottivastustuskyvyn levi\u00e4minen bakteerikantojen v\u00e4lill\u00e4 horisontaalisen\ngeeninsiirron avulla on merkitt\u00e4v\u00e4 ja kasvava maailmanlaajuinen ongelma\nihmisten terveydelle. Siirt\u00e4m\u00e4ll\u00e4 konjugaation avulla laajakirjoisia \u03b2-laktamaasi\n(ESBL)\u2013geenej\u00e4 tuottavia plasmideja vastustuskykyiset bakteerikannat pystyv\u00e4t\npelastamaan antibiooteille alttiita bakteereja my\u00f6s tappavassa\nantibioottialtistuksessa. T\u00e4m\u00e4 tutkimus pyrkii selvitt\u00e4m\u00e4\u00e4n mik\u00e4 on bakteerien\nymp\u00e4rist\u00f6n ravinnepitoisuuden vaikutus t\u00e4h\u00e4n pelastuskykyyn. Viitt\u00e4 eri ESBLplasmideja\nsis\u00e4lt\u00e4v\u00e4\u00e4 JM109 E.coli-kantaa kasvatettiin yhdess\u00e4 ampisilliinille alttiin\nHMS174 E.coli-kannan kanssa 5% ja 100% ravinnetasossa sek\u00e4 ilman antibioottia,\nett\u00e4 tappavassa ampisilliinikonsentraatiossa. Ampisilliinivastustuskyvyn\nsiirtymist\u00e4 ESBL-plasmidien mukana tutkittiin kasvattamalla n\u00e4ytteit\u00e4\nkasvatuksista ampisilliini\u00e4 ja rifampisiini\u00e4 sis\u00e4lt\u00e4vill\u00e4 maljoilla joilla vain ESBLplasmidin\nsis\u00e4lt\u00e4v\u00e4t HMS174 transkonjugantit pystyv\u00e4t kasvamaan. Tulokset\nosoittivat ett\u00e4 eri plasmidien kyky siirty\u00e4 bakteerikantojen v\u00e4lill\u00e4 vaihtelee\nmerkitt\u00e4v\u00e4sti. Ravinteiden m\u00e4\u00e4r\u00e4, antibioottivalinta ja plasmidien ominaisuudet,\nkuten niiden koko, vaikuttavat voimakkaasti konjugaatiotehokkuuteen.\nTutkimuksen tulokset voivat auttaa ymm\u00e4rt\u00e4m\u00e4\u00e4n antibioottiresistenssin\nevoluutiota ESBL-bakteerien infektoimissa potilaissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "The spread of antibiotic resistance between bacterial strains by plasmid-mediated\nconjugative horizontal gene transfer is a significant and growing global threat to\nhuman welfare. Resistant bacterial strains can rescue susceptible bacteria by\nconjugative transfer of extended spectrum \u03b2-lactamase (ESBL)-genes containing\nplasmids in lethal antibiotic concentrations. This study aims to investigate how the\nnutritional level of the environment effects this rescue potential. Five different\nESBL-plasmid containig JM109 E.coli-srains were grown together with an ampicillin\nsusceptible HMS174 E.coli-strain in 5% and 100% nutritional levels with and\nwithout antibiotic selection. The transfer of ampicillin resistance was studied by\ngrowing samples on plates containig ampicillin and rifampicin, on which only the\nESBL-plasmid containing HMS174 transconjugants could grow. The ability of these\nplasmids to transfer themselves conjugatively between bacterial strains varied\nsignificantly. The amount of nutrients, antibiotic selection and the properties of the\nplasmids, such as their size, have a stong effect on the efficiency of conjugational\ngene transfer. The results of the study may help to understand the evolution of\nresistance in patients infected by ESBL-bacteria.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2019-12-18T06:49:45Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2019-12-18T06:49:45Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "26", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "konjugatiivinen plasmidi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vastustuskykygeeni", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Ymp\u00e4rist\u00f6n ravinnepitoisuuden vaikutus konjugatiivisten ESBL-plasmidien kykyyn pelastaa antibiooteille alttiita bakteereja tappavassa antibioottipitoisuudessa horisontaalisen geeninsiirron avulla", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201912185369", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "plasmidit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "horisontaalinen geeninsiirto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "resistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ravinnepitoisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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