Patterns of thermal tolerance in Drosophila montana and D. flavomontana

Ympäristön lämpötila vaikuttaa suuresti eliöiden selviytymiseen, kasvuun ja lisääntymiseen. Hyönteiset voivat olla herkkiä ilmastossa tapahtuville muutoksille, koska niiden toimintakyky riippuu paljon ympäristön lämpötilasta. Hyönteisten erilaisten lämpötilojen sietämiseen liittyvät strategiat käsit...

Full description

Bibliographic Details
Main Author: Blomster, Anna
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2017
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/65973
_version_ 1826225770480009216
author Blomster, Anna
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Blomster, Anna Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Blomster, Anna Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Blomster, Anna
datasource_str_mv jyx
description Ympäristön lämpötila vaikuttaa suuresti eliöiden selviytymiseen, kasvuun ja lisääntymiseen. Hyönteiset voivat olla herkkiä ilmastossa tapahtuville muutoksille, koska niiden toimintakyky riippuu paljon ympäristön lämpötilasta. Hyönteisten erilaisten lämpötilojen sietämiseen liittyvät strategiat käsittävät elinkiertopiirteisiin, käyttäytymiseen ja fysiologiaan liittyviä ominaisuuksia, joista monet voivat muuttua geneettisen sopeutumisen ja/tai fenotyyppisen plastisuuden myötä. Tutkielmassani selvitin hyönteisten kuumuuden- ja kylmyydensietokykyä käyttäen tutkimuslajeina kahta lähisukuista mahlakärpäslajia, Drosophila montanaa ja D. flavomontanaa. Tutkimuksen tavoitteina oli selvittää kärpästen kuumuuden- ja kylmyydensietokyvyn lajienvälistä ja -sisäistä muuntelua sekä tutkia kylmäakklimoinnin vaikutusta lajien kylmyydensietokykyyn. Lisäksi tutkin, kuinka hyönteisten lämpötilatoleranssin mittaamisessa käytetyt ominaisuudet korreloivat keskenään, koska korrelaatiot voivat antaa viitteitä eri mittojen taustalla olevista fysiologisista mekanismeista. Lajien kuumuuden- ja kylmyydensietokyky selvitettiin mittaamalla kärpästen kriittinen lämpötilamaksimi, kriittinen lämpötilaminimi ja kylmähorroksesta toipumiseen kuluva aika. Tutkitut kärpäset olivat peräisin kärpäslinjoista, joiden perustajanaaraat oli kerätty eri puolilta Kalliovuorten aluetta Kanadasta ja Yhdysvalloista. Tulosten mukaan D. montanalla oli keskimäärin parempi kuumuuden- ja kylmyydensietokyky kuin D. flavomontanalla, ja mitatuissa ominaisuuksissa oli myös lajinsisäistä muuntelua. D. montana -naarailla oli keskimäärin parempi kuumuudensietokyky kuin lajin koirailla, kun taas D. flavomontanalla naaraiden ja koiraiden kuumuudensietokyvyissä ei ollut eroa. Kummallakaan lajilla ei löytynyt merkitsevää eroa naaraiden ja koiraiden kylmyydensietokyvyssä. Lisäksi tulosteni mukaan kummankin lajin kärpästen kylmyydensietokyky parani kylmäakklimoinnin seurauksena. D. flavomontanalla kriittisen lämpötilamaksimin ja kylmähorroksesta toipumiseen kuluvan ajan välillä oli merkitsevä negatiivinen korrelaatio, mutta muilta osin mitatut lämpötilatoleranssiominaisuudet eivät korreloineet keskenään merkitsevästi. Tutkimuksen tulokset tukivat aiempia tutkimustuloksia, joiden mukaan D. montana on hyvin kylmänkestävä Drosophila-laji. Lisäksi tämä tutkimus valotti vähemmän tutkitun lajin, D. flavomontanan, kuumuuden- ja kylmyydensietokykyyn liittyviä ominaisuuksia. Ymmärtääksemme miten eliöt reagoivat nopeasti muuttuviin ilmasto-olosuhteisiin, tarvitaan tietoa lajien lämpötilojen sietokykyyn liittyvistä strategioista. Tämä tutkimus selvitti näitä strategioita kahdella viileille alueille levinneellä hyönteislajilla. Environmental temperatures have a strong effect on the survival, growth and reproduction of organisms. Terrestrial insects are expected to be sensitive to changes in climate since their performance is strongly affected by environmental temperature. Thermal strategies of insect species are complex combinations of the traits related to life-history, behavior and physiology, and many of these traits can be modified by genetic adaptation and/or phenotypic plasticity. In my thesis, I studied the patterns of physiological thermal tolerance of insects using 2 closely-related fruit fly species, Drosophila montana and D. flavomontana, as model organisms. The aims of the study were to trace the patterns of basal heat and cold tolerances both between and within the study species and the effect of cold acclimation on flies’ cold tolerance. I also studied how different measures of heat and cold tolerance correlate with each other, as this can provide insights into the underlying mechanisms behind the different measures of insect thermal tolerance. Thermal tolerances of the flies were assessed by measuring flies’ critical thermal maxima (CTmax), critical thermal minima (CTmin) and chill coma recovery times (CCRT). The flies were derived from isofemale strains whose founder females had been collected from different parts of the Rocky Mountains area (Canada/USA). According to the results, D. montana was more tolerant both to heat and cold than D. flavomontana. In addition, I found intraspecific variation among and within populations in flies’ thermal tolerance traits. D. montana female flies had on average higher heat tolerance than males, whereas in D. flavomontana females and males did not differ from each other in their heat tolerances. Neither of the species showed sex differences in flies’ cold tolerance. My results also suggested that cold acclimation increases the cold tolerance of the flies in both species. Apart from the significant negative correlation between CTmax and CCRT in D. flavomontana, correlations between thermal tolerance measures were non-significant within both species. The results were in line with previous studies, which have shown that D. montana is a very cold tolerant Drosophila species with high cold acclimation capacity. In addition, this thesis described the patterns of thermal tolerance of much less studied species, D. flavomontana. In order to increase the understanding of the means that organisms and species have for coping with the rapidly changing climate, it is crucial to study the fundamentals of thermal strategies adopted by the species. This thesis described patterns of these strategies in insect species which have distributed to relatively cold areas.
first_indexed 2019-10-22T20:04:21Z
format Pro gradu
fullrecord [{"key": "dc.contributor.advisor", "value": "Kankare, Maaria", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Hoikkala, Anneli", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Blomster, Anna", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2019-10-22T06:22:35Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2019-10-22T06:22:35Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/65973", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ymp\u00e4rist\u00f6n l\u00e4mp\u00f6tila vaikuttaa suuresti eli\u00f6iden selviytymiseen, kasvuun ja lis\u00e4\u00e4ntymiseen.\nHy\u00f6nteiset voivat olla herkki\u00e4 ilmastossa tapahtuville muutoksille, koska niiden\ntoimintakyky riippuu paljon ymp\u00e4rist\u00f6n l\u00e4mp\u00f6tilasta. Hy\u00f6nteisten erilaisten l\u00e4mp\u00f6tilojen\nsiet\u00e4miseen liittyv\u00e4t strategiat k\u00e4sitt\u00e4v\u00e4t elinkiertopiirteisiin, k\u00e4ytt\u00e4ytymiseen ja\nfysiologiaan liittyvi\u00e4 ominaisuuksia, joista monet voivat muuttua geneettisen sopeutumisen\nja/tai fenotyyppisen plastisuuden my\u00f6t\u00e4. Tutkielmassani selvitin hy\u00f6nteisten kuumuuden- ja\nkylmyydensietokyky\u00e4 k\u00e4ytt\u00e4en tutkimuslajeina kahta l\u00e4hisukuista mahlak\u00e4rp\u00e4slajia,\nDrosophila montanaa ja D. flavomontanaa. Tutkimuksen tavoitteina oli selvitt\u00e4\u00e4 k\u00e4rp\u00e4sten\nkuumuuden- ja kylmyydensietokyvyn lajienv\u00e4list\u00e4 ja -sis\u00e4ist\u00e4 muuntelua sek\u00e4 tutkia\nkylm\u00e4akklimoinnin vaikutusta lajien kylmyydensietokykyyn. Lis\u00e4ksi tutkin, kuinka\nhy\u00f6nteisten l\u00e4mp\u00f6tilatoleranssin mittaamisessa k\u00e4ytetyt ominaisuudet korreloivat\nkesken\u00e4\u00e4n, koska korrelaatiot voivat antaa viitteit\u00e4 eri mittojen taustalla olevista\nfysiologisista mekanismeista. Lajien kuumuuden- ja kylmyydensietokyky selvitettiin\nmittaamalla k\u00e4rp\u00e4sten kriittinen l\u00e4mp\u00f6tilamaksimi, kriittinen l\u00e4mp\u00f6tilaminimi ja\nkylm\u00e4horroksesta toipumiseen kuluva aika. Tutkitut k\u00e4rp\u00e4set olivat per\u00e4isin k\u00e4rp\u00e4slinjoista,\njoiden perustajanaaraat oli ker\u00e4tty eri puolilta Kalliovuorten aluetta Kanadasta ja\nYhdysvalloista. Tulosten mukaan D. montanalla oli keskim\u00e4\u00e4rin parempi kuumuuden- ja\nkylmyydensietokyky kuin D. flavomontanalla, ja mitatuissa ominaisuuksissa oli my\u00f6s\nlajinsis\u00e4ist\u00e4 muuntelua. D. montana -naarailla oli keskim\u00e4\u00e4rin parempi\nkuumuudensietokyky kuin lajin koirailla, kun taas D. flavomontanalla naaraiden ja koiraiden\nkuumuudensietokyvyiss\u00e4 ei ollut eroa. Kummallakaan lajilla ei l\u00f6ytynyt merkitsev\u00e4\u00e4 eroa\nnaaraiden ja koiraiden kylmyydensietokyvyss\u00e4. Lis\u00e4ksi tulosteni mukaan kummankin lajin\nk\u00e4rp\u00e4sten kylmyydensietokyky parani kylm\u00e4akklimoinnin seurauksena. D. flavomontanalla\nkriittisen l\u00e4mp\u00f6tilamaksimin ja kylm\u00e4horroksesta toipumiseen kuluvan ajan v\u00e4lill\u00e4 oli\nmerkitsev\u00e4 negatiivinen korrelaatio, mutta muilta osin mitatut\nl\u00e4mp\u00f6tilatoleranssiominaisuudet eiv\u00e4t korreloineet kesken\u00e4\u00e4n merkitsev\u00e4sti. Tutkimuksen\ntulokset tukivat aiempia tutkimustuloksia, joiden mukaan D. montana on hyvin\nkylm\u00e4nkest\u00e4v\u00e4 Drosophila-laji. Lis\u00e4ksi t\u00e4m\u00e4 tutkimus valotti v\u00e4hemm\u00e4n tutkitun lajin, D.\nflavomontanan, kuumuuden- ja kylmyydensietokykyyn liittyvi\u00e4 ominaisuuksia.\nYmm\u00e4rt\u00e4\u00e4ksemme miten eli\u00f6t reagoivat nopeasti muuttuviin ilmasto-olosuhteisiin, tarvitaan\ntietoa lajien l\u00e4mp\u00f6tilojen sietokykyyn liittyvist\u00e4 strategioista. T\u00e4m\u00e4 tutkimus selvitti n\u00e4it\u00e4\nstrategioita kahdella viileille alueille levinneell\u00e4 hy\u00f6nteislajilla.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Environmental temperatures have a strong effect on the survival, growth and reproduction\nof organisms. Terrestrial insects are expected to be sensitive to changes in climate since their\nperformance is strongly affected by environmental temperature. Thermal strategies of insect\nspecies are complex combinations of the traits related to life-history, behavior and\nphysiology, and many of these traits can be modified by genetic adaptation and/or\nphenotypic plasticity. In my thesis, I studied the patterns of physiological thermal tolerance\nof insects using 2 closely-related fruit fly species, Drosophila montana and D. flavomontana,\nas model organisms. The aims of the study were to trace the patterns of basal heat and cold\ntolerances both between and within the study species and the effect of cold acclimation on\nflies\u2019 cold tolerance. I also studied how different measures of heat and cold tolerance\ncorrelate with each other, as this can provide insights into the underlying mechanisms behind\nthe different measures of insect thermal tolerance. Thermal tolerances of the flies were\nassessed by measuring flies\u2019 critical thermal maxima (CTmax), critical thermal minima\n(CTmin) and chill coma recovery times (CCRT). The flies were derived from isofemale\nstrains whose founder females had been collected from different parts of the Rocky\nMountains area (Canada/USA). According to the results, D. montana was more tolerant\nboth to heat and cold than D. flavomontana. In addition, I found intraspecific variation\namong and within populations in flies\u2019 thermal tolerance traits. D. montana female flies had\non average higher heat tolerance than males, whereas in D. flavomontana females and males\ndid not differ from each other in their heat tolerances. Neither of the species showed sex\ndifferences in flies\u2019 cold tolerance. My results also suggested that cold acclimation increases\nthe cold tolerance of the flies in both species. Apart from the significant negative correlation\nbetween CTmax and CCRT in D. flavomontana, correlations between thermal tolerance\nmeasures were non-significant within both species. The results were in line with previous\nstudies, which have shown that D. montana is a very cold tolerant Drosophila species with\nhigh cold acclimation capacity. In addition, this thesis described the patterns of thermal\ntolerance of much less studied species, D. flavomontana. In order to increase the\nunderstanding of the means that organisms and species have for coping with the rapidly\nchanging climate, it is crucial to study the fundamentals of thermal strategies adopted by the\nspecies. This thesis described patterns of these strategies in insect species which have\ndistributed to relatively cold areas.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2019-10-22T06:22:35Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2019-10-22T06:22:35Z (GMT). No. of bitstreams: 0\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "31", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "cold tolerance", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "heat tolerance", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "thermal stress", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "adaptation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Patterns of thermal tolerance in Drosophila montana and D. flavomontana", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201910224545", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ekologia ja evoluutiobiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ecology and evolutionary biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4011", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "akklimatisaatio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kylmyys", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hy\u00f6nteiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "l\u00e4mp\u00f6tila", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "acclimation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cold", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insects", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "temperature", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
id jyx.123456789_65973
language eng
last_indexed 2025-02-18T10:55:17Z
main_date 2017-01-01T00:00:00Z
main_date_str 2017
publishDate 2017
record_format qdc
source_str_mv jyx
spellingShingle Blomster, Anna Patterns of thermal tolerance in Drosophila montana and D. flavomontana cold tolerance heat tolerance thermal stress adaptation Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 akklimatisaatio kylmyys hyönteiset lämpötila acclimation cold insects temperature
title Patterns of thermal tolerance in Drosophila montana and D. flavomontana
title_full Patterns of thermal tolerance in Drosophila montana and D. flavomontana
title_fullStr Patterns of thermal tolerance in Drosophila montana and D. flavomontana Patterns of thermal tolerance in Drosophila montana and D. flavomontana
title_full_unstemmed Patterns of thermal tolerance in Drosophila montana and D. flavomontana Patterns of thermal tolerance in Drosophila montana and D. flavomontana
title_short Patterns of thermal tolerance in Drosophila montana and D. flavomontana
title_sort patterns of thermal tolerance in drosophila montana and d flavomontana
title_txtP Patterns of thermal tolerance in Drosophila montana and D. flavomontana
topic cold tolerance heat tolerance thermal stress adaptation Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 akklimatisaatio kylmyys hyönteiset lämpötila acclimation cold insects temperature
topic_facet 4011 Ecology and evolutionary biology Ekologia ja evoluutiobiologia acclimation adaptation akklimatisaatio cold cold tolerance heat tolerance hyönteiset insects kylmyys lämpötila temperature thermal stress
url https://jyx.jyu.fi/handle/123456789/65973 http://www.urn.fi/URN:NBN:fi:jyu-201910224545
work_keys_str_mv AT blomsteranna patternsofthermaltoleranceindrosophilamontanaanddflavomontana