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[{"key": "dc.contributor.advisor", "value": "Reshamwala, Dhanik", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kivist\u00f6, Eetu", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2019-10-14T06:05:35Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2019-10-14T06:05:35Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/65843", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Enterovirukset voivat aiheuttaa nuhamaisten oireiden lis\u00e4ksi vakavia, jopa kroonisia,\nsairauksia. Enteroviruksia vastaan ei ole saatavilla rokotteita, poliovirusta lukuun\nottamatta. Antiviraalisia l\u00e4\u00e4kkeit\u00e4 on kehitett\u00e4v\u00e4 virustartuntojen hoitoa varten.\nEndofyyttiuutteet ovat mahdollinen tutkimuskohde, joista viruksia vastaan tehoavia\naineita voisi l\u00f6yt\u00e4\u00e4. T\u00e4ss\u00e4 tutkimuksessa tutkimme endofyyttiuutteita, jotka olivat\naiemmin osoittautuneet tehokkaiksi coxsackieviruksia B3 ja A9 vastaan. Radioaktiivisia,\n35S \u2013merkittyj\u00e4 viruksia luotiin koetta varten, jossa tutkimme uutteiden vaikutusta\nvirusten kykyyn sitoutua solun pinnalle. Uutteilla ei havaittu olevan tilastollisesti\nmerkitt\u00e4v\u00e4\u00e4 sitoutumista h\u00e4iritsev\u00e4\u00e4 vaikutusta, mutta joissain tapauksissa ne lis\u00e4siv\u00e4t\nsitoutumista. Sytotoksisuuskokeilla m\u00e4\u00e4riteltiin uutteiden vaikutus solujen elinkykyyn.\nUutteet eiv\u00e4t osoittautuneet sytotoksisiksi. Soluja altistettiin uutteille ennen infektiota,\njotta mahdolliset uutteiden vaikutukset solujen sis\u00e4isiin mekanismeihin selvi\u00e4isiv\u00e4t.\nTuloksien perusteella uutteilla ei ollut vaikutusta t\u00e4ll\u00e4 tavalla. Vaikuttaa siis silt\u00e4, ett\u00e4\ntutkitut endofyyttiuutteet vaikuttavat suoraan viruspartikkeleihin ja t\u00e4ten est\u00e4v\u00e4t\ninfektiota. Jatkotutkimukset uutteiden sis\u00e4ll\u00f6ist\u00e4 ovat suositeltuja ja ne voisivat tarjota\nlis\u00e4tietoa niiden antiviraalisista mekanismeista.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Enteroviruses can cause acute and chronic infections. The only available vaccine is against poliovirus and vaccines cannot help if the infection is already present. As such, antivirals are needed to fight enterovirus infections. A potential source for novel antiviral compounds are endophyte extracts. In this experiment, we studied extracts from fungal endophytes that had previously shown antiviral activity against coxsackieviruses B3 and A9. The aim was to determine the antiviral target of the extracts. Radioactive viruses were created using 35S-labeled amino acids to be used in a binding assay. The binding assay showed that the extracts do not inhibit the binding of the virus on the cell surface and might even increase it in some cases. An ATP-assay was performed to determine the cytotoxicity of the extracts and all were found to be non-cytotoxic. Finally, a pretreatment experiment where cells were incubated with extract before infection was used to determine if host cell factors are affected to produce the antiviral effect. No such results were found and thus it is most likely that the extracts have an immediate effect on the virus capsid itself. Follow-up research on the currently unknown composition of the extracts will shed more light on this subject.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2019-10-14T06:05:34Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2019-10-14T06:05:35Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "35", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Endophytes", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "extract", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "antivirals", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Deciphering the antiviral mechanism of endophyte extract against coxsackieviruses B3 and A9", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201910144422", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virustaudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "viruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virus diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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