| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Siev\u00e4nen, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Permi, Perttu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Ojala, Alexandra", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2019-06-20T06:36:57Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2019-06-20T06:36:57Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/64745", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Tutkielman kirjallisessa osassa perehdyt\u00e4\u00e4n bakteerin solusein\u00e4n biosynteesin, peptidoglykaanin hajottamiseen, puhdistamiseen sek\u00e4 karakterisointiin. Kokeellisessa osassa valmistettiin kahta l\u00e4ht\u00f6ainetta, joita voidaan k\u00e4ytt\u00e4\u00e4 lysostafiinientsyymin siirtym\u00e4tila-analogin valmistamisessa. Valmistetutut komponentit olivat suojattu aminometyylihypofosforihapoke sek\u00e4 N-akryloyyliglysinaatti.\n\nPeptidoglykaanin biosynteesi\u00e4 k\u00e4yd\u00e4\u00e4n l\u00e4pi aloittaen sytoplasmassa tapahtuvista reaktioista siirtyen aikajanalla eteenp\u00e4in solukalvon sis\u00e4reunalla ja sen ulkopuolella tapahtuviin reaktioihin. Kussakin vaiheessa k\u00e4yd\u00e4\u00e4n l\u00e4pi reaktioita mahdollistavat entsyymit. Biosynteesin tarkastelu p\u00e4\u00e4tet\u00e4\u00e4n ristisiltoja muodostavien entsyymien toimintaan. Peptidoglykaanin hajottamisesta k\u00e4yd\u00e4\u00e4n l\u00e4pi niin autolysiinien kuin antibioottienkin toiminta. Useat antibiootit est\u00e4v\u00e4tkin juuri biosynteesiss\u00e4 vaikuttavien entsyymien toimintaan. \n\nPeptidoglykaanin tutkimuksesta perehdyt\u00e4\u00e4n peptidoglykaanin erist\u00e4miseen ja puhdistukseen, sek\u00e4 erilaisiin menetelmiin, joita k\u00e4ytt\u00e4en voidaan karakterisoida sen rakennetta. Usein peptidoglykaani erotetaan muista solusein\u00e4n osista ja hajotetaan liukenevaan muotoon. On my\u00f6s olemassa analyysimenetelmi\u00e4, joilla voidaan tutkia liukenematonta peptidoglykaania. Erikokoisia peptidoglykaanifragmentteja on my\u00f6s mahdollista valmistaa.\n\nPeptidoglykaanin rakenteen ja siihen vaikuttavien entsyymien tutkiminen on t\u00e4rke\u00e4\u00e4 sill\u00e4, ne ovat potentiaalisia kohteita esimerkiksi uusille antibiooteille. Entsyymej\u00e4 voidaan tutkia siirtym\u00e4tila-analogien avulla. Ymm\u00e4rt\u00e4m\u00e4ll\u00e4 entsyymien toimintamekanismeja on mahdollista kehitt\u00e4\u00e4 parempia inhibiittoreita tai uusia entsyymien kaltaisia molekyylej\u00e4, jotka voivat katalysoida haluttuja reaktiota.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2019-06-20T06:36:57Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2019-06-20T06:36:57Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "84", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "peptidoglykaani", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "siirtym\u00e4tila-analogi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Peptidoglykaanin hajottaminen, puhdistus ja karakterisointi", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201906203333", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Orgaaninen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Organic Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4035", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "entsyymit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solusein\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biosynteesi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hajotus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "puhdistus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|