Effect of temperature change on bacterial virulence

Abiotic factors such as temperature can influence the evolution of the pathogens, but empirical evidence on this is very scarce. The pathogen Serratia marcescens, which had evolved under three different temperature treatments for 1 month, and ancestor (ATCC 13880) of the evolved strains, were tested...

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Main Author: Chen, Zihan
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2018
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/60716
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author Chen, Zihan
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Chen, Zihan Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Chen, Zihan Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Chen, Zihan
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description Abiotic factors such as temperature can influence the evolution of the pathogens, but empirical evidence on this is very scarce. The pathogen Serratia marcescens, which had evolved under three different temperature treatments for 1 month, and ancestor (ATCC 13880) of the evolved strains, were tested for their virulence in Galleria mellonella- wax moth. Bacteria injected larvae were incubated in two different temperatures to explore phenotypic plasticity in virulence. The mortality of the hosts was recorded and analysed. Survival analysis with linear mixed-effects model in R revealed that temperature change affected the bacteria virulence. Ancestor and those strains that had evolved in constantly high 38°C temperature were found to exhibit high virulence whereas strains evolved in benign 31°C, constant or fluctuating (daily fluctuations between 24-38°C) were found to have attenuated virulence. Whole genome sequencing of evolved strains n=28 revealed putative and new virulence factors. My results show that high temperatures are able to maintain and select for genes increasing virulence. If my result reflects things in the wild and also in other pathogens results mean problems associated with increasing mean temperatures due to global change .
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The pathogen Serratia marcescens, which had evolved under three different temperature treatments for 1 month, and ancestor (ATCC 13880) of the evolved strains, were tested for their virulence in Galleria mellonella- wax moth. Bacteria injected larvae were incubated in two different temperatures to explore phenotypic plasticity in virulence. The mortality of the hosts was recorded and analysed. Survival analysis with linear mixed-effects model in R revealed that temperature change affected the bacteria virulence. Ancestor and those strains that had evolved in constantly high 38\u00b0C temperature were found to exhibit high virulence whereas strains evolved in benign 31\u00b0C, constant or fluctuating (daily fluctuations between 24-38\u00b0C) were found to have attenuated virulence. Whole genome sequencing of evolved strains n=28 revealed putative and new virulence factors. My results show that high temperatures are able to maintain and select for genes increasing virulence. 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spellingShingle Chen, Zihan Effect of temperature change on bacterial virulence Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 virulenssi geenit evoluutiobiologia lämpötila bakteerit taudinaiheuttajat infektiotaudit virulence genes evolutionary biology temperature bacteria pathogens infectious diseases
title Effect of temperature change on bacterial virulence
title_full Effect of temperature change on bacterial virulence
title_fullStr Effect of temperature change on bacterial virulence Effect of temperature change on bacterial virulence
title_full_unstemmed Effect of temperature change on bacterial virulence Effect of temperature change on bacterial virulence
title_short Effect of temperature change on bacterial virulence
title_sort effect of temperature change on bacterial virulence
title_txtP Effect of temperature change on bacterial virulence
topic Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 virulenssi geenit evoluutiobiologia lämpötila bakteerit taudinaiheuttajat infektiotaudit virulence genes evolutionary biology temperature bacteria pathogens infectious diseases
topic_facet 4011 Ecology and evolutionary biology Ekologia ja evoluutiobiologia bacteria bakteerit evolutionary biology evoluutiobiologia geenit genes infectious diseases infektiotaudit lämpötila pathogens taudinaiheuttajat temperature virulence virulenssi
url https://jyx.jyu.fi/handle/123456789/60716 http://www.urn.fi/URN:NBN:fi:jyu-201812205253
work_keys_str_mv AT chenzihan effectoftemperaturechangeonbacterialvirulence