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[{"key": "dc.contributor.advisor", "value": "Pentik\u00e4inen, Ulla", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Haataja, Tatu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Postila, Pekka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Salmikangas, Sami", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Roivas, Pekka", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-11-16T07:18:58Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-11-16T07:18:58Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2018", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/60200", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Monoamiinioksidaasi (MAO) on entsyymi, jonka kahta eri alamuotoa (MAO-A ja MAO-B)\nesiintyy mitokondrioiden ulkokalvoilla. MAO-entsyymit katalysoivat monoamiinien, kuten\ndopamiinin, serotoniinin ja adrenaliinin, oksidatiivista deaminaatiota. MAO-entsyymien\nliiallinen aktiivisuus hermosoluissa aiheuttaa muun muassa Parkinsonin- ja Alzheimerin\ntautia, dementiaa ja masennusta. MAO-B:t\u00e4 esiintyy l\u00e4hinn\u00e4 hermosoluissa, kun taas MAOA:ta\nesiintyy my\u00f6s sis\u00e4elimiss\u00e4, joten MAO-B:lle selektiiviset inhibiittorit ovat hyvi\u00e4\nl\u00e4\u00e4keaineita monien neurologisten tautien hoidossa. Joitain MAO-B selektiivisi\u00e4\ninhibiittoreita, kuten pargyliini\u00e4, k\u00e4ytet\u00e4\u00e4nkin jo yhdess\u00e4 l\u00e4\u00e4keaine Levodopan kanssa\nest\u00e4m\u00e4\u00e4n Parkinsonin tautiin liittyv\u00e4\u00e4 liiallista dopamiinin hajotusta aivoissa. N\u00e4in ollen\nuusien, tehokkaiden ja turvallisten MAO-B selektiivisten inhibiittorien l\u00f6yt\u00e4minen on hyvin\nt\u00e4rke\u00e4 aihe l\u00e4\u00e4keainetutkimuksessa. T\u00e4m\u00e4n ty\u00f6n tarkoituksena oli tutkia 23 uutta MAO-B\nselektiivist\u00e4 inhibiittoria ja niiden sitoutumis- ja inhibitiotehokkuutta MAO-B:n\nsitoutumistaskussa. Tutkimus toteutettiin sek\u00e4 tietokoneella tehdyll\u00e4 telakointisimulaatiolla\nett\u00e4 laboratoriossa suoritetulla fosfataasiaktiivisuusmittauksella. Tulokset osoittivat, ett\u00e4\ntestimolekyylien inhibitiotehokkuus ei ollut samalla tasolla kaupallisen pargyliiniinhibiittorin\nkanssa. Telakointien ja fosfataasiaktiivisuusmittausten tulokset kuitenkin\nauttavat edist\u00e4m\u00e4\u00e4n tulevaisuudessa teht\u00e4vien jatkotutkimusten menetelmien suunnittelua ja\ntoteuttamista. Tulokset auttavat my\u00f6s valitsemaan erilaisia inhibiittoreita tuleviin\njatkotutkimuksiin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Monoamine oxidase (MAO) is an enzyme that is expressed in two different isoforms (MAOA\nand MAO-B) on the outer membranes of mitochondria. MAOs catalyse the oxidative\ndeamination of monoamines such as dopamine, serotonin and adrenaline. The excessive\nactivity of MAOs in nerve cells cause Parkinson\u2019s- and Alzheimer\u2019s diseases, dementia,\ndepression and many other neurological disorders. MAO-B is predominantly expressed in\nnerve cells, but MAO-A is expressed also in the internal organs. This makes MAO-B\nselective inhibitors great drugs for the treatment of a variety of different neurological\ndisorders and diseases. Some MAO-B selective inhibitors, such as pargyline, are already\nbeing used alongside the drug Levodopa to prevent the Parkinson\u2019s disease associated\nexcessive deamination of dopamine in the brain. This means that discovering new, effective\nand safe MAO-B selective inhibitors is an important subject in drug research. The aim of\nthis study was to test the binding efficiency and inhibition efficiency of 23 new MAO-B\nselective inhibitors inside the binding site of MAO-B. The study was carried out by a docking\nsimulation using computer software, and also in practice in the form of a phosphatase activity\nassay in the laboratory. The results showed that the inhibition efficiency of the test molecules\nwere not on par with the commercial pargyline inhibitor. However, the results of the docking\nsimulations and phosphatase activity assays do help in planning and executing future studies\non the subject. The results also help in choosing different kinds of inhibitors for the future\nstudies.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2018-11-16T07:18:58Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-11-16T07:18:58Z (GMT). No. of bitstreams: 0\n Previous issue date: 2018", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "31", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "fosfataasiaktiivisuus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MAO-B", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "monoamiinioksidaasi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sitoutumistasku", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "telakointi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Uusien monoamiinioksidaasi B:n inhibiittorien hy\u00f6dynt\u00e4minen l\u00e4\u00e4keainetutkimuksessa", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "bachelor thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201811164747", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Bachelor's thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Kandidaatinty\u00f6", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_7a1f", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "bachelorThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "l\u00e4\u00e4kesuunnittelu", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "inhibiittorit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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