| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Haataja, Tatu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pentik\u00e4inen, Ulla", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Keisanen, Anni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-11-02T07:55:04Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-11-02T07:55:04Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2018", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/60085", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Aldo-keto-reduktaasi (AKR) kuuluu oksidoreduktaasin superperheeseen. Ihmisen\n(Homo sapiens) AKR1C3 (aldo-keto reduktaasi C3) s\u00e4\u00e4telee elimist\u00f6n\nhormonituotantoa osallistumalla prostaglandiini- ja steroidisynteeseihin. H\u00e4iri\u00f6t\nAKR1C3-geenin toiminnassa vaikuttavat ratkaisevasti erilaisten sairauksien, kuten\nhormoniriippuvaisten sy\u00f6pien syntymiseen. T\u00e4ll\u00e4 hetkell\u00e4 kliiniseen k\u00e4ytt\u00f6\u00f6n ei ole\nhyv\u00e4ksytty yht\u00e4\u00e4n AKR1C3-entsyymiin kohdennettua l\u00e4\u00e4keainetta. Tutkimuksen\ntavoitteena oli optimoida rekombinanttisen AKR1C3-proteiinin tuotto- ja\npuhdistusolosuhteet sek\u00e4 tutkia tietokonemallinnuksella suunniteltujen\nmolekyylien sitoutumista AKR1C3-entsyymiin. Tuotto- ja puhdistusmenetelmien\noptimoinnissa onnistuttiin saamaan 0.5 l tuotolla 11.9 mg 96.5 % puhdasta AKR1C3-\nproteiinia. Molekyylien sitoutumista AKR1C3-entsyymiin tutkittiin\nl\u00e4mp\u00f6tilariippuvaisella fluoresenssispektroskopialla. 64 tutkitusta molekyylist\u00e4 6\nmolekyyli\u00e4 sitoutui AKR1C3-entsyymiin. Molekyylien sitoutumismuodon\nm\u00e4\u00e4ritt\u00e4miseksi AKR1C3-entsyymi ja ligandi kiteytettiin riippuva pisara \u2013\nmenetelm\u00e4n sek\u00e4 liuotuskokeen avulla. Muodostuneista kompleksikiteiss\u00e4\nhavaittiin diffraktiota r\u00f6ntgenkristallografialla (MASSIF-1, ESRF). Tutkimuksessa\nl\u00f6ydettiin uusi suunta AKR1C3-kohdennetun l\u00e4\u00e4kkeen kehitt\u00e4miseen \u2013\nkumariinijohdannaiset ligandit.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2018-11-02T07:55:04Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-11-02T07:55:04Z (GMT). No. of bitstreams: 0\n Previous issue date: 2018", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "53", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "l\u00e4mp\u00f6tilariippuvainen fluoresenssispektroskopia", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "yhdistelm\u00e4DNA-tekniikka.", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "AKR1C3-entsyymin tuotto-, puhdistus- ja kiteytysolosuhteiden optimointi", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201811024606", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Biologian opettajankoulutus", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Teacher education programme in Biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4014", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "r\u00f6ntgenkristallografia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "l\u00e4\u00e4keaineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kumariinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kiteytys", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "entsyymit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|