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[{"key": "dc.contributor.advisor", "value": "Takala, Heikki", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Rossi, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Multam\u00e4ki, Elina", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-06-15T06:06:05Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-06-15T06:06:05Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2018", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/58571", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetiikan avulla voidaan kontrolloida tarkasti m\u00e4\u00e4riteltyj\u00e4 solun biologisia toimintoja k\u00e4ytt\u00e4en geneettisi\u00e4 ja optisia menetelmi\u00e4. T\u00e4m\u00e4n tutkimuksen tarkoituksena oli kehitt\u00e4\u00e4 ja testata punaiselle valolle herkk\u00e4 Deinococcus radiodurans -bakteerin fytokromiin (DrBphP) pohjautuva optogeneettinen ty\u00f6kalu. Ensimm\u00e4isen\u00e4 hypoteesina DrBphP:ll\u00e4 ja sen vasteregulaattorilla (DrRR) on punaisen valon aallonpituudella ohjattavaa nis\u00e4k\u00e4ssoluissa havaittavissa olevaa reversiibeli\u00e4 vuorovaikutusta. Toisena hypoteesina DrBphP monomeeri mutantti (FLmon) voidaan monomerisoida punaisen valon avulla ja j\u00e4lleen dimerisoida kaukopunaisella valotuksella ja t\u00e4t\u00e4 vuorovaikutusta voidaan havainnoida nis\u00e4k\u00e4ssoluissa. Kras4B lipidiankkuri (CAAX) tai tumalokalisaatiosignaali (engl. nuclear cap-binding protein subunit 1) kloonattiin DrRR- ja FLmon-inserttien C-terminaaliin. Vuorovaikutusta tutkittiin konfokaalimikroskoopilla kiinnitetyill\u00e4 ja el\u00e4vill\u00e4 soluilla transfektoiduissa HeLa-soluissa. DrBphP-DrRR vuorovaikutus on kvantitatiivisten analyysien perusteella voimakkaampaa pime\u00e4ss\u00e4 kuin valotettuna (p = 0,025). FLmon vuorovaikutusta ei pystytty havaitsemaan (p = 0,117). Tutkimuksia on syyt\u00e4 jatkaa tekem\u00e4ll\u00e4 stabiilit solulinjat. Lis\u00e4ksi mutaatioiden avulla pime\u00e4ss\u00e4 sitoutumista ja siten taustasignaalia voidaan pienent\u00e4\u00e4. Punainen valo pystyy tunkeutumaan syv\u00e4lle nis\u00e4k\u00e4skudokseen, joten punaiselle valolle herkk\u00e4 optogeneettinen systeemi, mahdollistaisi nis\u00e4k\u00e4ssolujen biologisten toimintojen tarkan s\u00e4\u00e4telyn noninvasiivisesti.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetics is a pioneer technique that allows targeted, rapid control of precisely defined events in complex biological systems with the help of genetic and optical methods. Aim of this study is to develop and test a red light switchable Deinococcus radiodurans \u2013bacterial phytochrome-based (DrBphP) optogenetic tool. The hypothesis stands that the interaction of DrBphP and its response regulator (DrRR) in mammalian cells is red light-inducible and reversible. The second hypothesis stands that DrBphP monomer mutant (FLmon) can monomerize with red light illumination and again dimerize with far-red light illumination and this interaction is observable in mammalian cells. Kras4B membrane motif (CAAX) or a nuclear localization signal of nuclear cap-binding protein subunit 1 (NLS) is cloned in to the C-terminus of the DrRR and FLmon inserts. The interactions are studied in transfected HeLa cells using confocal microscopy for both fixed and live cells. Confocal images demonstrate the functionality of translocation signals. DrBphP\u2013DrRR interaction is revealed by confocal images and quantitative analysis shows stronger intensity in dark versus light (p = 0.025). FLmon interaction is not observable by confocal microscope. Future directions are stable cell lines and mutations to lower background signal. Red light can penetrate deep into the mammalian tissue, therefore development of a red light-sensitive bacterial phytochrome-based optogenetic tool would offer noninvasive methods to regulate specific events in mammalian systems.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2018-06-15T06:06:04Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-06-15T06:06:05Z (GMT). No. of bitstreams: 0\n Previous issue date: 2018", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "56", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "fytokromi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mikroskopointi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "solukokeet", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "valoreseptori", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Bakteerifytokromipohjaisen optogeneettisen ty\u00f6kalun kloonaus ja testaus", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201806153227", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kloonaus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "soluviljely", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "molekyylibiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "valotus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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