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[{"key": "dc.contributor.advisor", "value": "Kalenius, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "V\u00e4h\u00e4talo, Sara", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-04-06T07:44:34Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-04-06T07:44:34Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2018", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1866887", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/57494", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "T\u00e4ss\u00e4 Pro gradu -tutkielmassa k\u00e4sitell\u00e4\u00e4n erilaisten organismien aineenvaihdunnan eli metabolian tutkimusta keskittyen erityisesti nis\u00e4kk\u00e4ille tyypilliseen soluhengitykseen ja haaraketjuisten aminohappojen aineenvaihduntaan. Haaraketjuisilla aminohapoilla on mekanismiltaan viel\u00e4 tuntematon yhteys lihaskasvuun ja rasvahappojen lis\u00e4\u00e4ntyneeseen aineenvaihduntaan. Kirjallisessa osassa tarkastellaan ravinnon makroravintoaineiden ja soluhengityksen yhteytt\u00e4, haaraketjuisten aminohappojen vaikutusta fyysiseen harjoitteluun, rasvahappoaineenvaihduntaan ja aineenvaihduntareitteihin Kainulainen et. al esitt\u00e4m\u00e4n mallin mukaisesti. T\u00e4t\u00e4 ehdotettua rasvahappojen hapettumisen ja haaraketjuisten aminohappojen katabolian v\u00e4list\u00e4 yhteytt\u00e4 on tarkoitus tutkia rotista, joilla on s\u00e4\u00e4delty ruokavalio, mutta tutkimusta tullaan tekem\u00e4\u00e4n my\u00f6s ihmissoluista. Aineenvaihduntareittien tutkimus tehd\u00e4\u00e4n nestekromatografia-massaspektrometria-menetelm\u00e4ll\u00e4 (LC-MS), jonka kehitt\u00e4mist\u00e4 varten tutkielman kokeellisessa osassa tehtiin kiinnostuksen kohteina olevien yhdisteiden ominaisuuksia kartoittavia massaspektrometrisia mittauksia. Kirjallisessa osuudessa perehdyt\u00e4\u00e4n nestekromatografia- ja kaasukromatografia-massaspektrometria-menetelmien ominaisuuksiin ja kehitykseen nimenomaan metabolomiikan n\u00e4k\u00f6kulmasta, mihin kuuluu n\u00e4ytteen esik\u00e4sittely, laitteiston ominaisuuksien s\u00e4\u00e4t\u00e4minen huippuunsa, k\u00e4ytettyjen liuottimien optimointi, niiden sopivien suhteiden l\u00f6yt\u00e4minen ja tulosten vertailu. Nestekromatografiasta, kaasukromatografiasta ja massaspektrometriasta esitet\u00e4\u00e4n teoreettiset perusteet ja vertaillaan niiden etuja metaboliittianalyysiss\u00e4.\r\nPro gradu -tutkielman kokeellinen osuus jakautui lopulta kahteen osaan: metaboliittianalyysi\u00e4 varten teht\u00e4v\u00e4\u00e4n massaspektrometriseen kartoitukseen ja kukurbit[n]uriilien (n = 6, 7, 8) (CB[n]) ja (2, 3-diatsabisyklo[2.2.2]okt-2-en-1-yyli)metyyliamiini-vierasmolekyylin vuorovaikutuksen massaspektrometriseen tutkimukseen. Metaboliittien massaspektrometrisissa kokeissa havaittiin odotetusti negatiivisen polarisaation spektreiss\u00e4 selke\u00e4sti tunnistettavat piikit ja voimakkaammat intensiteetit l\u00e4hes kaikille tutkittaville yhd\u00edsteille verrattuna positiivisen polarisaation spektreihin. Suurin osa tutkimukseen kuuluvista metaboliiteista oli poolisia ja sis\u00e4lsiv\u00e4t karboksyylihapporyhm\u00e4n. Ainoastaan oksaloetikkahappoa ei onnistuttu analysoimaan massaspektrometrisesti. Kaikista muista metaboliiteista eristettiin [M-H]- -ioni, jolle tehtiin t\u00f6rm\u00e4ysaktivointikokeet. T\u00f6rm\u00e4ysaktivointikokeiden perusteella LC-MS-analyysiin saadaan lis\u00e4\u00e4 luotettavuutta ja niist\u00e4 saatavan informaation pohjalta isobaariset yhdisteet pystyt\u00e4\u00e4n erottamaan.\r\nKukurbituriilien massaspektrometrisissa analyyseiss\u00e4 tarkkailtiin vapaan atsoalkaanivieraan hajoamista ja verrattiin sit\u00e4 CB[n]:n ja atsoalkaanin komplekseissa tapahtuvaan hajoamiseen. Analysointi tehtiin happamissa olosuhteissa. Analyyseiss\u00e4 havaittiin atsoalkaanin pilkkoutuvan eri tavoin is\u00e4nt\u00e4molekyyliin sitoutuneena verrattuna vapaan atsoalkaanimolekyylin pilkkoutumiseen. Monimuotoisimmat vuorovaikutukset ja pilkkoutumiset havaittiin CB[7]:n ja atsoalkaanin kompleksien pilkkoutumisessa. CB[6] on onkaloltaan liian pieni sitomaan atsoalkaania sis\u00e4\u00e4ns\u00e4 ja CB[8]:n onkalo on niin suuri, ett\u00e4 vieraana oleva atsoalkaani irtoaa siit\u00e4 helpommin kuin CB[7]:n onkalosta, joten pilkkoutumista havaitaan v\u00e4hemm\u00e4n, kuin CB[7]:n ja atsoalkaanin komplekseissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Sara V\u00e4h\u00e4talo (salivaha) on 2018-04-06 07:44:33.555926. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2018-04-06T07:44:34Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201804061934.pdf: 3358073 bytes, checksum: 35adfefb7bea1cf22632c5083118d09a (MD5)\r\nlicense.html: 4795 bytes, checksum: d461026fd0de64ac8d669cc6ceff7938 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-04-06T07:44:34Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201804061934.pdf: 3358073 bytes, checksum: 35adfefb7bea1cf22632c5083118d09a (MD5)\r\nlicense.html: 4795 bytes, checksum: d461026fd0de64ac8d669cc6ceff7938 (MD5)\r\n Previous issue date: 2018", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (119 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "BCAA", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", 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