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[{"key": "dc.contributor.advisor", "value": "H\u00e4iv\u00e4, Veli-Mies", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Myllyniemi, Maria", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-03-19T09:59:23Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-03-19T09:59:23Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1862488", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/57348", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Helicobacter pylori \u2013infektio on riskitekij\u00e4 useiden sairauksien kuten pohjukaissuolen haavauman ja ma-hasy\u00f6v\u00e4n synnyss\u00e4. Maastricht V/Firenze -konsensusraportti suosittelee testaa-ja-hoida -strategiaa ja no-ninvasiivisten testien k\u00e4ytt\u00f6\u00e4 nuoremmille potilaille, joilla ei ole h\u00e4lytt\u00e4vi\u00e4 oireita. Nopeiden, hoitopaikka-testaukseen soveltuvien serologisten testien k\u00e4ytt\u00f6\u00e4 ei kuitenkaan niiden vaihtelevan suorituskyvyn vuoksi suositella. Tutkimuksen tavoitteena oli arvioida mahdollisuutta kehitt\u00e4\u00e4 kaupallinen, hoitopaikkatestauk-seen ja h\u00e4\u00e4t\u00f6hoidon seurantaan soveltuva kvantitatiivinen H. pylori IgG/IgA/IgM-lateksiagglutinaatiotesti Orion Diagnostican 1990 \u2013luvun lopun QuikRead-testiformaatille. Eri kokoisia lateksipartikkeleita p\u00e4\u00e4llys-tettiin kovalenttisesti H. pylori \u2013antigeenill\u00e4, ja immunoagglutinaatioreaktion tasoa mitattiin turbidometri-sesti QuikRead-instrumentilla 654 ja 950 nm aallonpituuksilla, 10 min reaktioajalla. Alustavat p\u00e4\u00e4llystys- ja testiolosuhteet m\u00e4\u00e4ritettiin keskikokoisella partikkelilla, ja testin diagnostista herkkyytt\u00e4, spesifisyytt\u00e4 ja dynaamista mittausaluetta arvioitiin vertaamalla seerumin\u00e4ytteiden tuloksia referenssitestien (Pyloriset Dry, Pyloriset IgG ELISA) tiedettyihin arvoihin. Ter\u00e4vin absorbanssin muutos saavutettiin k\u00e4ytett\u00e4ess\u00e4 654 nm:n mittausaallonpituutta ja l\u00e4hell\u00e4 keskitasoa olevia partikkelikokoja. Mittausalueen yl\u00e4p\u00e4\u00e4ss\u00e4 korkein reak-tion taso (dA654nm ~ 0,9) havaittiin keskikokoisella partikkelilla, mutta herkkyys matalien positiivisten n\u00e4yt-teiden m\u00e4\u00e4rityksess\u00e4 oli riitt\u00e4m\u00e4t\u00f6n. Pieni parannus herkkyydess\u00e4 saatiin aikaan nostamalla partikkelin kokoa ja edelleen, n\u00e4ytetilavuutta optimaalisesta 15 \u00b5l:sta 25 \u00b5l:an. N\u00e4ill\u00e4 muutoksilla p\u00e4\u00e4stiin l\u00e4helle tavoiteltua herkkyytt\u00e4 suhteessa referenssitesteihin, mutta reaktion taso j\u00e4i matalaksi (dA654nm < 0,05) suh-teessa arvioituun cut-off -pitoisuuteen. Havaittu dynaaminen mittausalue kapeni korkeita vasta-ainepitoisuuksia sis\u00e4lt\u00e4vill\u00e4 n\u00e4ytteill\u00e4 titrausvasteen muuttuessa ei-lineaariseksi. Tulosten perusteella testi-metodi soveltui H. pylori -vasta-aineiden kvantitatiiviseen mittaukseen, mutta havaittu kapea dynaaminen mittausalue yhdistettyn\u00e4 arvioon testin matalasta herkkyydest\u00e4 viittasivat ei-optimaaliseen suorituskykyyn H. pylori -infektion luotettavassa diagnostiikassa ja hoidonseurannassa. Lis\u00e4ksi ty\u00f6ss\u00e4 havaitut k\u00e4yt\u00e4nn\u00f6n ongelmat liittyen p\u00e4\u00e4llystetyn partikkelin huonoon s\u00e4ilyvyyteen, toistettavuuteen, sek\u00e4 partikkelien, anti-geenin ja n\u00e4ytteen k\u00e4ytettyihin korkeisiin pitoisuuksiin vaativat lis\u00e4optimointia. Reaktio-olosuhteiden ja erityisesti p\u00e4\u00e4llystysmenetelm\u00e4n optimoinneilla voidaan olettaa saavutettavan parannusta suorituskykyyn, mutta olemassa olevilla tiedoilla ty\u00f6n laajuutta ja optimoinnin lopputulosta on vaikea arvioida. Luotettava arvio menetelm\u00e4n soveltuvuudesta toiminnallisen, kaupallisen testin kehitykseen vaatii siten laajempaa tutkimusta ja optimointia kuin t\u00e4ss\u00e4 ty\u00f6ss\u00e4 on tehty.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Helicobacter pylori infection is a risk factor for several diseases, including duodenal ulcer and gastric cancer. A test-and-treat strategy using noninvasive tests is recommended by the Maastricht V/Florence Consensus Report for younger patients without alarming symptoms. The use of serological, rapid point-of-care tests, however, is not recommended due to their varied performance. The aim of the study was to assess the feasi-bility of a commercial, quantitative H. pylori IgG/IgA/IgM latex agglutination test for Orion Diagnostica's QuikRead test format from the end of 1990 decade to be used for point-of-care testing and monitoring the eradication of H. pylori. Latex particles of varied sizes were covalently coated with H. pylori antigen, and the rate of immunoagglutination reaction was measured turbidometrically by a QuikRead-instrument, at measuring wavelengths of 654 and 950 nm and 10 min reaction time. Preliminary coating and test condi-tions were defined using medium-sized particles, and diagnostic sensitivity, specificity and dynamic range was assessed comparing the results of the serum samples to the known values of reference tests (Pyloriset Dry, Pyloriset IgG ELISA). The sharpest change in absorbance was achieved by using measuring wavelength of 654 nm and particle sizes close to the medium level. At the high end of the measuring range, the highest level of reaction (dA654nm ~ 0,9) was observed with medium-size particles, but the sensitivity in detecting low positive samples was insufficient. A small improvement in sensitivity was accomplished by increasing the particle size and furthermore, the sample volume from optimal 15 \u00b5l to 25 \u00b5l. With these changes, a sensitiv-ity close to that of the reference tests was achieved, but the reaction rate observed was low (dA654nm <0,05) compared to the estimated cut-off value. The observed dynamic range was narrowed due to a non-linear titration response of samples with high antibody concentrations. Based on the results, the test method was found suitable for quantitative measurement of H. pylori antibodies, but the observed narrow dynamic measuring range, together with the estimated low sensitivity, implied non-optimal performance in reliable H. pylori diagnostics and eradication monitoring. In addition, observed practical problems concerning the low stability and repeatability of coated particles and high concentrations of particles, antigen and sample used would require larger re-optimization. Optimization of reaction conditions, especially the coating method, can be assumed to improve the performance, but based on the existing data, the extent and final outcome of the optimization is difficult to estimate. The final assessment of the feasibility of the method for viable, com-mercial product would therefore require a larger study and optimization that was done in this work.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Maria Myllyniemi (mamyllyn) on 2018-03-19 09:59:22.884472. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =False]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2018-03-19T09:59:23Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201803191755.pdf: 869683 bytes, checksum: 58240f1920fc0cdf9410a2519cffa652 (MD5)\nlicense.html: 1224 bytes, checksum: 8c87fb1b678dd24fb1a3a143147d2c70 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-03-19T09:59:23Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201803191755.pdf: 869683 bytes, checksum: 58240f1920fc0cdf9410a2519cffa652 (MD5)\nlicense.html: 1224 bytes, checksum: 8c87fb1b678dd24fb1a3a143147d2c70 (MD5)\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (51 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Helicobacter pylori", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lateksin immunoagglutinaatio", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "IgG", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "IgA", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "IgM", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "turbidometria", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Lateksin immunoagglutinaatioon perustuvan menetelm\u00e4n soveltuvuus Helicobacter pylori -vasta-aineiden nopeaan, kvantitatiiviseen m\u00e4\u00e4ritykseen", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": 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Aineisto on luettavissa Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6asemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "This material has a restricted access due to copyright reasons. It can be read at the workstation at Jyv\u00e4skyl\u00e4 University Library reserved for the use of archival materials: https://kirjasto.jyu.fi/en/workspaces/facilities.", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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