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[{"key": "dc.contributor.advisor", "value": "Vihinen-Ranta, Maija", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Hakanen, Satu", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2018-01-03T08:38:38Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2018-01-03T08:38:38Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1809781", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/56574", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Herpes simplex virus 1 (HSV-1) on alfaherpesviruksiin kuuluva vaipallinen, kaksijuosteista DNA:ta sis\u00e4lt\u00e4v\u00e4 virus. Kosketus- ja pisaratartuntana herk\u00e4sti levi\u00e4v\u00e4 HSV-1 on maailmanlaajuisesti huomattava taudinaiheuttaja, sill\u00e4 virusta kantaa arviolta 67 % v\u00e4est\u00f6st\u00e4. Tartunta on elinik\u00e4inen. Latentissa vaiheessa HSV-1:n genomi s\u00e4ilyy aistihermojen hermosolmukkeissa. Viruksen aktivoituminen lyyttiseen lis\u00e4\u00e4ntymiskiertoon oireilee tyypillisesti rakkuloina kasvojen iholla ja limakalvoilla. HSV-1:n genomin replikoituminen ja uusien viruskapsidien muodostuminen tapahtuvat is\u00e4nt\u00e4solun tumassa, jossa viruksen rakentumisalue laajenee infektion edetess\u00e4. Samalla is\u00e4nt\u00e4solun kromatiini tiivistyy ja pakkautuu tuman reunamille. Uudet viruskapsidit poistuvat tumasta kuroutumalla tumakalvon l\u00e4pi mekanismilla, joka tunnetaan jo varsin hyvin. Sen sijaan on viel\u00e4 ep\u00e4selv\u00e4\u00e4, kuinka kapsidit saavuttavat sisemm\u00e4n tumakalvon liikkuessaan kromatiinin seassa. T\u00e4ss\u00e4 tutkimuksessa m\u00e4\u00e4ritettiin is\u00e4nt\u00e4solun kromatiinin ominaisuuksia ja vuorovaikutuksia viruksen kanssa lyyttisen HSV-1-infektion my\u00f6h\u00e4isess\u00e4 vaiheessa. L\u00f6yh\u00e4sti pakkautuneen aktiivisen eukromatiinin ja tiiviimmin pakkautuneen hiljaisen heterokromatiinin erottamiseen k\u00e4ytettiin vasta-ainemarkkereita, jotka sitoutuvat kromatiinin eri muodoille ominaisiin histonimodifikaatioihin. Konfokaali- ja elektronimikrokooppikuvauksen perusteella infektoituneen solun kromatiini oli tiivistynyt tuman reunoille ep\u00e4s\u00e4\u00e4nn\u00f6llisen muotoiseksi renkaaksi. Tiivistynytt\u00e4 kromatiinirengasta katkoivat paikoitellen sisemm\u00e4lle tumakalvolle ulottuvat aukkokohdat, joiden kautta viruskapsidien n\u00e4htiin p\u00e4\u00e4tyv\u00e4n tumakalvon l\u00e4heisyyteen. Tihe\u00e4mm\u00e4n kromatiinin sekaan sijoittuneiden viruskapsidien ymp\u00e4rill\u00e4 n\u00e4htiin elektronimikroskooppikuvauksessa usein s\u00e4dekeh\u00e4n kaltainen tyhj\u00e4 tila, joka voisi selitty\u00e4 kapsidin ja kromatiinin v\u00e4lisill\u00e4 vuorovaikutuksilla. Kromatiinin eri muotojen \nsijoittuminen muuttui infektiossa siten, ett\u00e4 eukromatiinia havaittiin tumakalvon vieress\u00e4 ns. periferaalisella alueella. Periferaalinen eukromatiini saattaa viitata viruksen aikaansaamiin muutoksiin geenitoiminnassa. Lyyttisell\u00e4 HSV-1-infektiolla on merkitt\u00e4v\u00e4 vaikutus is\u00e4nt\u00e4solun tumaan ja kromatiinin rakenteeseen. Kromatiinin ja viruksen v\u00e4listen vuorovaikutusten tarkempi m\u00e4\u00e4ritt\u00e4minen voi tuoda lis\u00e4tietoa kapsidien tumansis\u00e4isist\u00e4 vaiheista ja tarjota samalla potentiaalisia kohteita l\u00e4\u00e4kemolekyyleille, joilla infektion eteneminen pyrit\u00e4\u00e4n pys\u00e4ytt\u00e4m\u00e4\u00e4n tumaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Herpes simplex virus 1 (HSV-1) is an enveloped icosahedral dsDNA virus, and a common human pathogen with an estimated incidence of 67% in the global population. Infected individuals permanently carry the latent HSV-1 in neuronal cells. Sporadic virus reactivation into the lytic life cycle typically manifests as facial lesions known as cold sores. HSV-1 utilizes the host cell nucleus for genome replication and nucleocapsid assembly. Nuclear interior is occupied by viral replication compartments, whereas the host chromatin becomes compressed and marginalized to the nuclear periphery. Progeny nucleocapsids exit the intact nucleus by budding through the nuclear envelope. While the mechanism of nuclear egress has been well-characterized, less is \nknown about progeny nucleocapsid movement among the host chromatin to the inner nuclear membrane. The aim of this study was to observe the properties of host chromatin in relation to viral replication compartments and progeny nucleocapsids during the late phase of lytic HSV-1 infection. In order to gain a more detailed look into the host chromatin as a structural element, we studied the redistribution of the loosely packed active euchromatin and the compacted silent heterochromatin by labeling their respective histone post-translational modifications. Confocal and transmission electron microscopy (TEM) imaging of HSV-1 infected nuclei showed that the marginalized host chromatin forms an uneven ring, which contains gaps that allow progeny nucleocapsids to access the inner nuclear membrane for egress. Nanometer-scale visualization of nucleocapsids in TEM images revealed that nucleocapsids are frequently surrounded by an electron-lucent, halo-like space, which might be related to nucleocapsid movement through the host chromatin. Notably, active euchromatin appears in the nuclear periphery adjacent to the inner nuclear membrane upon infection, although it is yet unclear if this finding represents virally activated euchromatin. Chromatin remodeling is a prominent feature of lytic HSV-1 infection. Further characterization of the key interactions between host chromatin and viral components could expose critical steps in HSV-1 nuclear egress, which might have additional value as therapeutic targets of this widespread human virus.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Satu Hakanen (saanhaka) on 2018-01-03 08:38:37.395086. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2018-01-03T08:38:38Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201801031024.pdf: 2048735 bytes, checksum: 2fd5e1825af155478d78d416c4cf256d (MD5)\nlicense.html: 4811 bytes, checksum: 71144c77bc3a632344eee416d11e252d (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2018-01-03T08:38:38Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201801031024.pdf: 2048735 bytes, checksum: 2fd5e1825af155478d78d416c4cf256d (MD5)\nlicense.html: 4811 bytes, checksum: 71144c77bc3a632344eee416d11e252d (MD5)\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (42 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "HSV-1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "herpesvirukset", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tuma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kromatiini", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "virus-tuma-vuorovaikutukset", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Virus-nucleus interactions in late herpesvirus infection", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201801031024", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2018-01-03T08:38:38Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "herpes simplex -virus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "herpesvirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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