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[{"key": "dc.contributor.advisor", "value": "Kallioniemi, Anne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ampuja, Minna", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lappetel\u00e4inen, Johanna", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2017-12-27T14:19:00Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2017-12-27T14:19:00Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1809864", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/56548", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Rintasy\u00f6p\u00e4 on johtava naisten sy\u00f6p\u00e4kuolemien aiheuttaja maailmanlaajuisesti. Luun\nmorfogeneettiset proteiinit (BMP) ovat tranformoiva kasvutekij\u00e4 beta- superperheeseen\nkuuluvia proteiineja. BMP:den tiedet\u00e4\u00e4n olevan osallisina monissa sy\u00f6p\u00e4tyypeiss\u00e4.\nRintasy\u00f6v\u00e4ss\u00e4 BMP4:ll\u00e4 on merkitt\u00e4vi\u00e4 vaikutuksia solukasvuun, liikkuvuuteen ja\nlevinn\u00e4isyyteen. Aiempien tutkimusten mukaan BMP4:n on havaittu laskevan solukasvua\nmerkitt\u00e4v\u00e4sti rintasy\u00f6p\u00e4soluilla. T\u00e4m\u00e4n tutkimuksen kohteena oli kaksi BMP4:n\nkohdegeeni\u00e4, ID2 (Inhibitor of DNA binding 2) ja SKIL eli SKI-like proto-onkogeeni.\nHypoteesina oli selvitt\u00e4\u00e4 johtuuko BMP4:n vaikutukset solukasvuun ID2- tai SKIL-geenist\u00e4.\nBT-474 ja T-47D rintasy\u00f6p\u00e4soluja k\u00e4siteltiin ihmisen rekombinantti proteiini BMP4:lla tai\npelk\u00e4ll\u00e4 kuljettimella ( BMP4:n liuoitusneste). ID2 ja SKIL hiljennettiin RNA h\u00e4irinn\u00e4n\navulla k\u00e4ytt\u00e4en geeneihin kohdennettuja siRNAa: siSKIL, siID2 ja kontrollina\nlusiferaasigeenin hiljent\u00e4v\u00e4 siLUC. Hiljent\u00e4misen vaikutuksia solukasvuun m\u00e4\u00e4ritettiin\nsolulaskuin kahdessa aikapisteess\u00e4. Geenien hiljentyminen varmistettiin RT-qPCR:ll\u00e4.\nBMP4 laski solukasvua BT-474 ja T-47D soluilla merkitt\u00e4v\u00e4sti. BMP4:n vaikutukset olivat\nyhtenev\u00e4iset ryhm\u00e4n aiempien tutkimusten kanssa. Solulaskujen perusteella geenien\nhiljent\u00e4misell\u00e4 ei ollut vaikutusta solukasvuun. Tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4\novatko kyseiset geenit osallisena BMP4:n aiheuttamaan solukasvun alenemiseen. Tulokset\nosoittivat etteiv\u00e4t ID2 tai SKIL ainakaan yksist\u00e4\u00e4n ole vastuussa BMP4:n vaikutuksista\nsolukasvuun.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Breast cancer is the leading cause of cancer deaths among women worldwide. Bone\nmorphogenetic proteins (BMPs) are a protein family that belongs to transforming growth\nfactor \u03b2 superfamily. BMPs are involved in many cancer types. In the case of breast cancer\nBMP4 has a significant influence on cell growth, migration, and invasion. Previous studies\nhave shown that BMP4 decreased cell growth of breast cancer cells significantly. The aim\nof this study was to examine two BMP4 target genes, Inhibitor of DNA binding 2 (ID2) and\nSKI-like proto-oncogene (SKIL). The hypothesis was that ID2 and SKIL were involved in\nthe effects of BMP4 on cell growth. Breast cancer cells BT-474 and T-47D were treated with\nhuman recombinant BMP4 or vehicle (0,1 % BSA in 4 mM HCl). ID2 and SKIL were\nsilenced by RNA interference using the targeted siRNAs; siSKIL, siID2 and a control was\nsiLUC (luciferase gene). Effects of silencing on cell growth were followed by cell counting\nin two time points. Gene silencing was confirmed by qPCR. BMP4 decreased the growth of\nBT-474 and T-47D cells significantly. Effects of BMP4 were consistent with previous\nstudies. Results of cell counting showed that the silencing of the genes did not affect cell\ngrowth. In conclusion, the aim was to observe whether the BMP4 target genes were involved\nin the BMP4 -induced decrease of cell growth in breast cancer cells. The results showed\nBMP4 decreased cell growth but they also showed that ID2 or SKIL alone does not to affect\ncell growth.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Johanna Lappetel\u00e4inen (sijolapp) on 2017-12-27 14:18:59.752223. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2017-12-27T14:19:00Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201712274887.pdf: 924075 bytes, checksum: 10de335fcbd7d481f7e844a500187574 (MD5)\nlicense.html: 4868 bytes, checksum: 5605712082f6664e44b202889b8d9b47 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2017-12-27T14:19:00Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201712274887.pdf: 924075 bytes, checksum: 10de335fcbd7d481f7e844a500187574 (MD5)\nlicense.html: 4868 bytes, checksum: 5605712082f6664e44b202889b8d9b47 (MD5)\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (49 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "BMP", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "BMP4", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "ID2", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "SKIL", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Luun morfogeneettisen proteiini 4:n kohdegeenien SKIL ja ID2 vaikutukset rintasy\u00f6p\u00e4soluihin", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201712274887", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2017-12-27T14:19:00Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rintasy\u00f6p\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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