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[{"key": "dc.contributor.author", "value": "Takanen, Anna", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2017-05-02T12:32:25Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2017-05-02T12:32:25Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1700279", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/53737", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Syd\u00e4ntaudit ovat johtava kuolinsyy maailmanlaajuisesti, ja siksi potilasspesifisien l\u00e4\u00e4ketestausmallien sek\u00e4\r\nkudosregeneraation tutkimus on ajankohtainen aihe. Nykyinen teknologia ei pysty viel\u00e4 tuottamaan suuria\r\nm\u00e4\u00e4ri\u00e4 puhtaita ihmisen indusoiduista pluripotenteista kantasoluista (hiPSC) erilaistettuja syd\u00e4nsoluja\r\ntarpeeksi tehokkaasti, jotta niit\u00e4 voitaisiin k\u00e4ytt\u00e4\u00e4 regeneratiivisessa l\u00e4\u00e4ketieteess\u00e4 ja luotettavammassa\r\nl\u00e4\u00e4ketestauksessa. Syd\u00e4nsolujen tuottamista ja karakterisointia t\u00e4ytyy kehitt\u00e4\u00e4. T\u00e4m\u00e4n tutkimuksen tavoite oli\r\njatkaa potilasspesifisten hiPSC syd\u00e4nsolujen erilaistamisen karakterisoimista ja testata oletetun syd\u00e4nsolujen\r\nerilaistusta tehostavan pienmolekyylin (PCDE) vaikutusta erilaistamisen tehokkuuteen. Hypoteesi oli, ett\u00e4\r\nkyseinen pienmolekyyli parantaisi erilaistustehokkuutta. Erilaistustehokkuutta mitattiin virtaussytometrisesti\r\nsyd\u00e4nmarkkerilla, Troponiin T:ll\u00e4, ja kardiomyosiitit karakterisoitiin immunov\u00e4rj\u00e4yksill\u00e4 sek\u00e4 kvantitatiivisen\r\npolymeraasiketjureaktion (qPCR) avulla. Geeniekspressiota analysoitiin RNA-n\u00e4ytteist\u00e4 erilaistuksen eri\r\nvaiheista. Tutkimuksen aikana arvioitiin tekniikkana videopohjaista yksitt\u00e4isten solujen sykeanalyysia, joka on\r\ntietokoneohjelmaan pohjautuva tukeva tutkimusmenetelm\u00e4 yksitt\u00e4isten solujen sykekuvion m\u00e4\u00e4ritt\u00e4miseksi.\r\nTuloksiin perustuen PCDE ei onnistunut tehostamaan hiPSC syd\u00e4nsolujen erilaistamista ja saattoi v\u00e4hent\u00e4\u00e4\r\nsyd\u00e4nmarkkereiden ekspressiota. Totesimme kuitenkin, ett\u00e4 videopohjaista sykeanalyysi\u00e4 voitaisiin k\u00e4ytt\u00e4\u00e4\r\nyhdess\u00e4 yksitt\u00e4isten solujen PCR-tutkimusten kanssa syd\u00e4nsolujen paremman karakterisoinnin\r\nsaavuttamiseksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Cardiac disease remains the leading cause of death in the world. Therefore, the need for patient specific drug\r\ntesting models, as well as tissue regeneration, are hot topics in medicine. The modern technology is still unable\r\nto efficiently produce pure cultures of human induced stem cell (hiPSC) derived cardiomyocytes, which are\r\nneeded for several purposes including regenerative medicine and reliable drug testing for cardiac disease.\r\nFurther development of hiPSC derived cardiomyocyte differentiation and characterization techniques is\r\nrequired. The objective of this experiment was to further characterize the differentiation of hiPSC derived\r\ncardiomyocytes obtained from patients and to test the effects of a novel putative cardiac differentiation\r\nenhancing small molecule (PCDE), on the efficiency of differentiation. The hypothesis was that the small\r\nmolecule would increase the differentiation efficiency. The efficiency was tested with flow cytometry using a\r\ncardiac marker, Troponin T, and the cardiomyocytes were characterized by immunostaining and quantitative\r\npolymerase chain reaction (qPCR). Gene expression was analyzed from RNA samples collected from different\r\ntime points of differentiation. During the experiment video-based beating analysis, a robust technique that\r\nutilizes a computer program to characterize beating patterns of single cardiomyocytes, was evaluated as a\r\ntechnique. Based on all results PCDE failed to enhance the differentiation efficiency of hiPSC derived\r\ncardiomyocytes and may have reduced the expression of cardiac markers. However, we found that the video\r\nbased beating analysis could be used in combination with single cell PCR to better characterize\r\ncardiomyocytes.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Anna Takanen (anjutaka) on 2017-05-02 12:32:25.261834. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2017-05-02T12:32:25Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201705022145.pdf: 1358376 bytes, checksum: cbe7cc5a259b5f462a3757ff4be635b9 (MD5)\r\nlicense.html: 4857 bytes, checksum: 47bd3f51d46f8031aafef51378969d5d (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2017-05-02T12:32:25Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201705022145.pdf: 1358376 bytes, checksum: cbe7cc5a259b5f462a3757ff4be635b9 (MD5)\r\nlicense.html: 4857 bytes, checksum: 47bd3f51d46f8031aafef51378969d5d (MD5)\r\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (41 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Novel small molecule in differentiation of hiPSC derived cardiomyocytes and single cell beating analysis", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201705022145", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2017-05-02T12:32:26Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kantasolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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