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[{"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Runtuvuori, Anniina", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2017-03-28T07:11:46Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2017-03-28T07:11:46Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1690696", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/53385", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Coxsackievirusryhm\u00e4 B (CVB) kuuluu Picornaviridae -heimoon ja Enterovirus-sukuun. Pikornavirukset ovat pieni\u00e4, halkaisijaltaan noin 30 nm, vaipattomia ja ikosahedraalisia viruksia. Viimeaikaiset tutkimukset ovat osoittaneet, ett\u00e4 CVB-ryhm\u00e4\u00e4n kuuluvat virukset voivat edesauttaa tyypin 1 diabeteksen (T1D) kehittymisess\u00e4. Vaikka enterovirusten sek\u00e4 T1D:n kehittymisen v\u00e4lill\u00e4 on selke\u00e4 yhteys, virusten T1D:n aiheuttavaa mekanismia ei viel\u00e4 tunneta. Hypoteesimme oli, ett\u00e4 enterovirusinfektio aiheuttaa hiiren haimakudoksen tuhoutumista ja voimme paikantaa viruksen kuoriproteiineja n\u00e4ist\u00e4 kudoksista tehdyist\u00e4 ohutleikkeist\u00e4 vasta-aineiden sek\u00e4 cryo-immuno-elektronimikroskopian avulla. Hiiren haimakudokset altistettiin kolmen p\u00e4iv\u00e4n ajan Coxsackievirus B1:lle (CVB1) ja B3:lle (CVB3). Hiiren haiman\u00e4ytteet tuotettiin ja toimitettiin JDRF nPOD-Virus yhteisty\u00f6ryhm\u00e4n toimesta (el\u00e4inkokeet hyv\u00e4ksytty eettisen toimikunnan toimesta: R13028). T\u00e4ss\u00e4 tutkimuksessa, haiman n\u00e4ytteet tutkittiin k\u00e4ytt\u00e4en l\u00e4p\u00e4isyelektronimikroskooppia (TEM). TEMin avulla tutkittiin virusten aiheuttaman kudostuho ja virusten sijainti haimassa. Virukset leimattiin ohuilta j\u00e4\u00e4leikkeilt\u00e4 enterovirusten vasta-aineilla ja 10 nm ja 5 nm proteiini A. kullalla. Infektoituneet solut osoittivat apoptoosin merkkej\u00e4. Lis\u00e4ksi havaittiin virusten aiheuttamia kalvovesikkeleiden muutoksia sek\u00e4 tummia kalvorakenteita soluissa. Vasta-aineet tunnistivat infektoitujen solujen solulimasta syntyneet uudet virusproteiinit, kun ei-infektoiduissa n\u00e4ytteiss\u00e4 oli vain v\u00e4h\u00e4n taustasignaalia. Tulokset osoittavat, ett\u00e4 CVB1 ja CVB3 aiheuttavat rakenteellisia muutoksia ja apoptoosia hiiren haimakudoksissa. Tulosten perusteella, virusta valmistui sek\u00e4 eksokriini- ett\u00e4 endokriinikudoksissa. T\u00e4m\u00e4n tutkimuksen tuloksia voidaan hy\u00f6dynt\u00e4\u00e4 tutkittaessa enterovirusten aiheuttamia haimavaurioita.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Coxsackievirus group B (CVB) belongs to the family of Picornaviridae and to the genus of Enterovirus. Picornaviruses are small, approximately 30 nm in diameter, non-enveloped, spherical and icosahedral viruses. Recent studies have indicated that viruses belonging to the Coxsackievirus group B contribute to the emergence of type 1 diabetes (T1D). Although there is a correlation between the viral infections and T1D, the mechanism of the viral pathogenesis in the development of diabetes is not fully known yet. Our hypothesis was that enterovirus infection causes destruction in the mouse pancreatic tissue and we can detect the viral capsid proteins from the ultrathin sections with cryo-immuno-EM. Mice were exposed (3 days) to Coxsackievirus B1 (CVB1) and Coxsackievirus B3 (CVB3). Mice pancreas samples were provided and submitted by the JDRF nPOD-Virus Group (under ethical approvement R13028). In this study, pancreatic samples were examined using transmission electron microscopy (TEM). With TEM, the tissue damage level, caused by the viruses, and location of the viruses was examined. The viruses were labeled on ultrathin cryosections with anti-enterovirus antibodies and 10 nm and 5 nm protein A gold particles. The cells showed signs of apoptosis. In addition, modified membrane vesicles associated with viruses and virus induced dark membrane structures were observed. The antibodies detected newly formed virus particles and capsid proteins sensitively in the cytoplasm of infected cells with low background in non-infected cells. The results show that CVB1 and CVB3 cause structural changes and apoptosis in the pancreas tissue and viruses are produced in both exocrine and endocrine structures. The results of this study can be utilized while exploring the reasons to pancreatic damage caused by enterovirus.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Anniina Runtuvuori (samaanru) on 2017-03-28 07:11:45.821796. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =False]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2017-03-28T07:11:46Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201703281773.pdf: 3863830 bytes, checksum: a936768f31d2c7e751977a99311b73d4 (MD5)\r\nlicense.html: 1126 bytes, checksum: 6917fc783cbe990b8d0e829059678bb7 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2017-03-28T07:11:46Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201703281773.pdf: 3863830 bytes, checksum: a936768f31d2c7e751977a99311b73d4 (MD5)\r\nlicense.html: 1126 bytes, checksum: 6917fc783cbe990b8d0e829059678bb7 (MD5)\r\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (42 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Coxsackievirus B1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Coxsackievirus B3", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Detection of enterovirus infection in pancreas tissue", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201703281773", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2017-03-28T07:11:47Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "nuoruustyypin diabetes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "elektronimikroskoopit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enterovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Aineistoon p\u00e4\u00e4sy\u00e4 on rajoitettu tekij\u00e4noikeussyist\u00e4. Aineisto on luettavissa Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6asemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "This material has a restricted access due to copyright reasons. It can be read at the workstation at Jyv\u00e4skyl\u00e4 University Library reserved for the use of archival materials: https://kirjasto.jyu.fi/en/workspaces/facilities.", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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