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[{"key": "dc.contributor.advisor", "value": "Turkki, Paula", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Matilainen, Johanna", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-09-14T12:29:08Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-09-14T12:29:08Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2016", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1574676", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/51364", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Aggresomin muodostuminen on yksi solun puolustusmekanismeista, joka pyrkii est\u00e4m\u00e4\u00e4n proteiinien haitallisen aggregoitumisen solulimassa. Aikaisemmat kokeet ovat n\u00e4ytt\u00e4neet, ett\u00e4 ihmisen enterovirus-infektio aiheuttaa muutoksia vimentiiniss\u00e4, tyypin III v\u00e4likokoisessa filamentissa (Turkki et al., julkaisematon data). N\u00e4m\u00e4 muutokset ovat hyvin samankaltaisia aggresomin muodostumisen aikana tapahtuvien muutoksien kanssa. T\u00e4m\u00e4n tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4 mahdollisen aggresomin muodostumisreitin aktivoituminen Coxsackievirus B3 (CVB3) -infektiossa, ja hypoteesimme oli, ett\u00e4 h\u00e4kkim\u00e4isten vimentiinirakenteiden muodostumisen lis\u00e4ksi my\u00f6s muita aggresomin muodostumisreitin aktivoitumiseen yhdistettyj\u00e4 tapahtumia voidaan havaita CVB3-infektoiduissa soluissa. Lis\u00e4ksi arvioimme, ett\u00e4 aggresomin muodostumisreitin aktivoituminen voi hy\u00f6dytt\u00e4\u00e4 virusinfektiota tai is\u00e4nt\u00e4solua. Tutkimme ty\u00f6ss\u00e4 n\u00e4it\u00e4 aggresomin muodostumiseen liittyvi\u00e4 solumuutoksia infektoiduissa soluissa k\u00e4ytt\u00e4m\u00e4ll\u00e4 immunofluoresenssiv\u00e4rj\u00e4ystekniikoita ja konfokaalimikroskopiaa. Tuloksemme osoittivat, ett\u00e4 CVB3-infektio aiheuttaa h\u00e4kkim\u00e4isen vimentiinirakenteen muodostumisen, ja ett\u00e4 t\u00e4m\u00e4 rakenteellinen muutos vaatii mikrotubuluksia. Infektio aiheuttaa lis\u00e4ksi suuria rakenteellisia muutoksia solulimakalvostoon ja Golgin laitteeseen, sek\u00e4 ubikitiinin ja l\u00e4mp\u00f6shokki (Hsp) 70 proteiinin siirtymisen perinukleaariseen tilaan. Tuloksemme viittaavat siihen, ett\u00e4 aggresomireitti voi aktivoitua CVB3-infektiossa. Tietojemme mukaan aggresomireitin aktivoitumista ei ole aikaisemmin osoitettu tapahtuvan enterovirusten aiheuttamien infektioiden aikana. Vaikka enteroviruksia on tutkittu kauan, niiden lis\u00e4\u00e4ntymiskierron kaikkia eri vaiheita ei tunneta t\u00e4ysin. Tutkimuksemme tulokset tarjoavat uutta tietoa enterovirus-is\u00e4nt\u00e4solu \u2013vuorovaikutuksista.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Aggresome formation is a cellular defence mechanism that is triggered in order to prevent the accumulation of aggregated proteins in the cytoplasm. It has been previously shown that human enterovirus infection causes changes in vimentin, type III intermediate filament protein (Turkki et al., unpublished data), and these structural changes in vimentin resemble the ones induced during aggresome formation. The aim of this study was to investigate the possible activation of aggresomal pathway during Coxsackievirus B3 (CVB3) infection. The hypothesis was that in addition to a vimentin cage, also other factors of the aggresomal pathway might be involved in viral infection, either for the benefit of the virus or host cell. We investigated these cellular changes by using immunofluorescent techniques and confocal microscope imaging. Our results illustrated that CVB3 infection causes a cage-like vimentin, and this rearrangement is dependent on microtubules. In addition, CVB3 infection was shown to induce major structural changes in the ER and Golgi apparatus, accompanied by the translocation of ubiquitin and Heat shock protein (Hsp) 70 to the perinuclear region. These observations indicated that aggresomal pathway could be involved in CVB3 infection. To our best knowledge, the utilization of aggresomal machinery by enteroviruses has not been shown earlier. Despite the fact that enteroviruses have been under research for a long time, there are still areas of the viral lifecycle that are poorly known. Our findings provide more information about enterovirus-host cell \u2013interactions.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Johanna Matilainen (matijoma) on 2016-09-14 12:29:07.627528. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-09-14T12:29:08Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201609144108.pdf: 2031259 bytes, checksum: 7665e7c8fa8607a05ff6475f78dc41ac (MD5)\r\nlicense.html: 4833 bytes, checksum: 167a46ebcc1bb560cadda3b52f82fefe (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-09-14T12:29:08Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201609144108.pdf: 2031259 bytes, checksum: 7665e7c8fa8607a05ff6475f78dc41ac (MD5)\r\nlicense.html: 4833 bytes, checksum: 167a46ebcc1bb560cadda3b52f82fefe (MD5)\r\n Previous issue date: 2016", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (50 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "aggresomi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vimentiini", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Coxsackievirus B3", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "aggresome", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "ER stress", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vimentin", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The activation of aggresomal pathway in Coxsackievirus B3 infection", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": 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"language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-09-14T12:29:08Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": 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