| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Narkilahti, Susanna", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4-Outinen, Laura", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "K\u00e4rn\u00e4, Paula", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-06-22T17:21:44Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-06-22T17:21:44Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2016", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1545479", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/50577", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Solunsiirtohoitoja voidaan tulevaisuudessa mahdollisesti k\u00e4ytt\u00e4\u00e4 neurorappeumasairauksien, kuten Parkinsonin ja Alzheimerin tautien, hoitoon. Ihmisen kantasolut ovat suuren erilaistumiskykyns\u00e4 vuoksi yksi lupaava hoitomuoto. Paljon tutkimuksia kuitenkin vaaditaan, ennen kuin kantasolunsiirtohoitoja voidaan tehd\u00e4 ihmisille. Toimivan siirteen edellytyksen\u00e4 on, ett\u00e4 se pysyy elinkykyisen\u00e4 ja pystyy integroitumaan olemassa olevaan hermostoon. S\u00e4hk\u00f6isen stimulaation on osoitettu lis\u00e4\u00e4v\u00e4n hermoverkkojen aktiivisuutta ja parantavan niiden uusiutumiskyky\u00e4 sek\u00e4 in vitro ett\u00e4 in vivo. T\u00e4ss\u00e4 opinn\u00e4ytety\u00f6ss\u00e4 tutkittiin miten matalataajuinen s\u00e4hk\u00f6inen stimulaatio vaikuttaa alkion kantasoluista erilaistettujen hermosolujen kypsymiseen.\n\nAlkion kantasolut erilaistettiin hermosoluiksi menetelm\u00e4ll\u00e4, joka oli aikaisemmin optimoitu meid\u00e4n tutkimusryhm\u00e4ss\u00e4mme. Hermosolut siirrostettiin mikroelektrodihiloille (MEA), joiden avulla voitiin mitata hermoverkkojen aktiivisuutta. Hermosolujen annettiin kasvaa mikroelektrodien p\u00e4\u00e4ll\u00e4 yhdest\u00e4 kolmeen viikkoa, jonka j\u00e4lkeen niit\u00e4 stimuloitiin s\u00e4hk\u00f6isesti. Stimulaatiossa k\u00e4ytettiin ohjelmoitavaa erikoisvalmisteista stimulaatiolaitteistoa. Testasimme erilaisia stimulaatioparametrej\u00e4, joille oli yhteist\u00e4 sama amplitudi ja pulssin leveys, jotka olivat \u00b1800 mV ja 2 ms. Muut parametrit vaihtelivat riippuen kokeesta. Kokeilimme stimulaatiota k\u00e4ytt\u00e4m\u00e4ll\u00e4 frekvenssej\u00e4 1, 5 ja 20 Hz. Stimulaation kesto vaihteli 21 tunnin ja 72 tunnin v\u00e4lill\u00e4, lis\u00e4ksi stimulaation intensiteetti vaihteli eri kokeissa. Stimulaation j\u00e4lkeen hermoverkon aktiivisuutta mitattiin MEA-tekniikalla. RT-PCR-analyysill\u00e4 tutkittiin stimulaation vaikutusta geenitasolla.\n\nTulokset osoittivat, ett\u00e4 5 Hz stimulaatio nosti hermoverkon aktiivisuutta, kun taas 20 Hz stimulaatio laski aktiivisuutta. 1 Hz stimulaatiolla ei ollut vaikutusta tai vaihtoehtoisesti se saattoi hieman laskea hermoverkon aktiivisuutta. Mitk\u00e4\u00e4n n\u00e4ist\u00e4 muutoksista eiv\u00e4t kuitenkaan olleet pysyvi\u00e4. Muutaman p\u00e4iv\u00e4n stimulaation p\u00e4\u00e4ttymisen j\u00e4lkeen, stimuloitujen verkkojen aktiivisuus palautui samalle tasolle kuin kontrolliverkkojen aktiivisuus. RT-PCR-tulokset osoittivat, ett\u00e4 kypsien hermosolujen markkerit, mictorubule-associated protein 2 ja neurofilament 68, hiljeniv\u00e4t 20 Hz stimulaation j\u00e4lkeen.\n\nTulokset viittaavat siihen, ett\u00e4 matalataajuinen stimulaatio k\u00e4ytetyill\u00e4 parametreill\u00e4 ei aiheuta pysyvi\u00e4 muutoksia hermoverkoston aktiivisuudessa ja siten se ei tue kehittyv\u00e4n hermoverkoston kypsymist\u00e4. T\u00e4m\u00e4 voi tarkoittaa sit\u00e4, ett\u00e4 alkion kantasoluista erilaistetut hermoverkot eiv\u00e4t ole tarpeeksi kehittyneit\u00e4, tai vaihtoehtoisesti ne ovat liian muuntautumiskykyisi\u00e4, jotta ne voisivat antaa pysyv\u00e4n vasteen solun ulkoiseen stimulaatioon. Tulokset osoittivat, ett\u00e4 matalataajuinen stimulaatio ei ole tehokas tapa edist\u00e4\u00e4 hermoverkkojen kypsymist\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Cell transplantation therapies may be used to treat neurodegenerative diseases, such as Parkinson's and Alzheimer's diseases, in the future. Human embryonic stem cells are one potential option to treat such diseases because of their great differentiation potential. However, much research is required before stem cell transplantation therapy can be used to treat humans. In order to get functional transplant, it must remain viable and have the ability to integrate into the nervous system. Electrical stimulation has been shown to evoke neuronal activity and improve nerve regeneration in vitro and in vivo. In this thesis, we investigated how the low-frequency electrical stimulation effects on the maturation of the human embryonic stem cell-derived neuronal cells.\n\nHuman embryonic stem cells were differentiated to neuronal cells by using the protocol which was earlier optimized by our group. The neuronal cells were plated on microelectrode arrays (MEA), which allowed measuring of spontaneous network activities. The neuronal cells were allowed to grow on top of microelectrodes for one to three weeks before stimulation. The stimulation was performed with the custom-built programmable stimulation system. We tested various stimulation parameters, which all consisted of the same amplitude of \u00b1800 mV and pulse width of 2 ms. Other parameters varied depending on the experiment. We tested the frequencies of 1, 5, and 20 Hz and stimulation durations between 21 h to 72 h and also various stimulation intensities. After stimulation, the electrical activity of neurons was monitored using microelectrode arrays. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the effects of stimulation on gene level.\n\nWe found out that the 5 Hz stimulation increased the network activity, while the 20 Hz stimulation decreased the activity. The 1 Hz stimulation either had no effect or it decreased the network activity slightly. However, none of the changes were permanent. A few days after the stimulation, the activity of stimulated networks recovered to the same level as the activity of control networks. According to the RT-PCR results, the mature neuronal markers, microtubule-associated protein 2 and neurofilament 68, were silenced after the 20 Hz stimulation.\n\nThese findings suggest that low-frequency stimulation, with used parameters, does not cause permanent changes in networks activity and thus does not promote maturation of developing neural networks. This can mean that hESC -derived neuronal cells are not mature enough, or they are too plastic that they could respond to extracellular stimulation. Also high-frequency stimulation could produce longer effects than low-frequency stimulation. Our results suggested that low-frequency stimulation is not an effective way to promote neuronal network maturation.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Paula K\u00e4rn\u00e4 (paankarn) on 2016-06-22 17:21:43.808275. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =False]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-06-22T17:21:44Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201606223334.pdf: 4553279 bytes, checksum: 44f1d79a10eecbd0ff08e0e07b17c356 (MD5)\nlicense.html: 1151 bytes, checksum: fb9f7abe7749fe7ec91c302ebceba9db (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-06-22T17:21:44Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201606223334.pdf: 4553279 bytes, checksum: 44f1d79a10eecbd0ff08e0e07b17c356 (MD5)\nlicense.html: 1151 bytes, checksum: fb9f7abe7749fe7ec91c302ebceba9db (MD5)\n Previous issue date: 2016", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (65 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "electrical stimulation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "microelectrode array", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "neuronal cell", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "stem cell", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "s\u00e4hk\u00f6inen stimulaatio", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mikroelektrodihila", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "hermosolu", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kantasolu", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Effect of low-frequency stimulation on the maturation of neuronal networks in vitro", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201606223334", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-06-22T17:21:45Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "stimulointi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hermosolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kantasolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Aineistoon p\u00e4\u00e4sy\u00e4 on rajoitettu tekij\u00e4noikeussyist\u00e4. Aineisto on luettavissa Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6asemalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "This material has a restricted access due to copyright reasons. It can be read at the workstation at Jyv\u00e4skyl\u00e4 University Library reserved for the use of archival materials: https://kirjasto.jyu.fi/en/workspaces/facilities.", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|