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[{"key": "dc.contributor.advisor", "value": "Ihalainen, Teemu", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Ij\u00e4s, Tommi", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-06-19T18:33:55Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-06-19T18:33:55Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2016", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1545121", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/50437", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "T\u00e4m\u00e4 tutkimus keskittyi analysoimaan, onko solun ulkopuolisen ymp\u00e4rist\u00f6n j\u00e4ykkyydell\u00e4 vaikutusta tuman\nsis\u00e4isten proteiinien jakautumiseen. Tuman mekanosensitiivisyyden vaikutusta lamiini A:n ja aktiinin\nsijaintiin tumassa tutkittiin soluilla, joita kasvatettiin eri j\u00e4ykkyisill\u00e4 kasvualustoilla. Kokeissa laskettiin\nlamiini A:n suhteellinen m\u00e4\u00e4r\u00e4 vertaamalla tumalevyyn sitoutunutta ja tumassa vapaana olevaa lamiini A:ta,\nsek\u00e4 suhteellinen m\u00e4\u00e4r\u00e4 aktiinille vertaamalla tumansis\u00e4isen aktiinin m\u00e4\u00e4r\u00e4\u00e4 solulimassa olevaan. Kahta eri\nj\u00e4ykkyist\u00e4 (1,5 kPa ja 33 kPa Youngin moduluksella) polyakryyliamidi (PAA)-geeli\u00e4 k\u00e4ytettiin s\u00e4\u00e4dett\u00e4v\u00e4n\u00e4\nkasvualustana soluille.\nN\u00e4ill\u00e4 geeleill\u00e4 kasvatettiin hiiren 3T3 fibroblastisoluja, joihin oli transfektoitu konstrukti, joka ilment\u00e4\u00e4 joko\nlamiini A:ta tai aktiinia tehostetulla vihre\u00e4ll\u00e4 fluoresoivalla proteiinilla (EGFP) leimattuna. Proteiineja\nilment\u00e4vi\u00e4 soluja kuvannettiin laser-skannaus konfokaalimikroskoopilla. Data k\u00e4siteltiin ja analysoitiin\nimageJ-ohjelmalla ja lopulliset tulokset saatiin vertaamalla tuman sis\u00e4ist\u00e4 vapaata lamiini A:ta tumalevyyn\nsistoutuneeseen, sek\u00e4 aktiinille vertaamalla tuman sis\u00e4isen aktiinin intensiteetti\u00e4 solulimasta saatuun\nintensiteettiin.\nLamiini A:lle tulokset n\u00e4yttiv\u00e4t, ett\u00e4 suurempi j\u00e4ykkyys vaikuttaa lamiini A:n dynamiikkaan suurentamalla\ntumalimassa vapaana olevan lamiini A:n m\u00e4\u00e4r\u00e4\u00e4 suhteessa tumalevyyn sitoutuneeseen. Aktiinille tulokset\nolivat v\u00e4hemm\u00e4n yhtenevi\u00e4 tutkimuksen aikana, mutta my\u00f6s tumansis\u00e4isen aktiinin m\u00e4\u00e4r\u00e4 vaikuttaisi\nkasvavan j\u00e4ykemm\u00e4ll\u00e4 alustalla suhteutettuna soluliman aktiinipitoisuuteen. Lamiini A:n suhteen on\nkuitenkin n\u00e4ytetty ett\u00e4 sen kokonaispitoisuus kasvaa ja fosforyloidun lamiini A:n m\u00e4\u00e4r\u00e4 v\u00e4henee kun solu on\nj\u00e4ykemm\u00e4ss\u00e4 ymp\u00e4rist\u00f6ss\u00e4. Vaikka n\u00e4m\u00e4 tulokset eiv\u00e4t suoranaisesti tuekkaan toisiaan, osoitettiin\ntutkimuksessa alustan j\u00e4ykkyyden vaikuttavan sitoutuneen ja vapaan lamiini A:n suhteeseen. Aktiinista\nsaadut tulokset tukevat tietoja aktiinin aktiivisen tumakuljetuksen kasvamisesta kun solu joutuu rasituksen\nalaiseksi. Molemmat tulokset kuitenkin osoittavat j\u00e4ykkyydell\u00e4 olevan suora vaikutus tuman proteiinien\ndynamiikkaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "In this study we aimed to see if the rigidity of the extracellular environment can influence protein distribution\nwithin the nucleus. The effect of nuclear mechanosensing on the spatial localization of lamin A and actin was\nstudied in cells cultured on different rigidity cell culture substrates. We quantified the relative levels of lamin\nA in nuclear lamina vs. nucleoplasm and the amount of intranuclear actin vs. cytoplasmic actin. Two different\nrigidities (1.5 kPa and 33 kPa of Young's modulus) of polyacrylamide (PAA)-gels were used as a tunable\nsubstrate for the cells.\nOn these gels, we cultured mouse 3T3 fibroblast cells, which were transfected with enhanced green\nfluorescent protein (EGFP) labeled lamin A and actin. Cultured cells we imaged with laser scanning confocal\nmicroscope. Images were processed and analyzed using imageJ and comparisons were made between mean\nintensities of lamin A in the nuclear lamina and nucleoplasm, and between intranuclear and cytoplasmic\nactin.\nWith lamin A, the results showed that a higher rigidity affects lamin A dynamics, by a significant increase of\nfree lamin A in relation to bound lamin A. With actin, the results were more unclear, but the same trend\nseems to continue. Actin amount inside nucleus seemed to increase related to cytoplasmic actin, when the\ncells were cultured on more rigid substrate. In both cases, substrate rigidity affected the protein dynamics by\nincreasing free protein inside nucleus. Actin also seems to be actively taken into nucleus in larger quantities\nin more rigid environment. It has been recently shown that stiffer substrate increases lamin A amount and\ndecreases phosphorylated lamin A, which might contradict our results. The effect of substrate rigidity on\nactin regulation is still poorly understood, but our results seem to be in line with proposed actin active intake\nto nucleus at cellular stress. Still, both of these results signify that cells have more direct mechanisms of\nsensing inside nucleus to affect nuclear activities.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Tommi Ij\u00e4s (toilijas) on 2016-06-19 18:33:55.025852. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-06-19T18:33:55Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201606193209.pdf: 2310312 bytes, checksum: 3125f2b5e8661fad7ad94eae626594e4 (MD5)\nlicense.html: 4825 bytes, checksum: 90e16a8eddd5967c68ded34a86c9f3f3 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-06-19T18:33:55Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201606193209.pdf: 2310312 bytes, checksum: 3125f2b5e8661fad7ad94eae626594e4 (MD5)\nlicense.html: 4825 bytes, checksum: 90e16a8eddd5967c68ded34a86c9f3f3 (MD5)\n Previous issue date: 2016", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (39 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "lamiini A", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "polyakryyliamidi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Effect of substrate rigidity on distribution of nuclear actin and lamin A", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201606193209", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-06-19T18:33:56Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aktiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "j\u00e4ykkyys", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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