| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Pihko, Petri", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Siitonen, Juha", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "K\u00e4rki, Katja", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-06-02T06:41:02Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-06-02T06:41:02Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2016", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1542670", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/50041", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ty\u00f6n kirjallisessa osassa k\u00e4sitell\u00e4\u00e4n ketonien enantioselektiivist\u00e4 pelkistyst\u00e4 entsyymeill\u00e4. Entsymaattiset reaktiot ovat kiinnostavia hyv\u00e4n selektiivisyytens\u00e4 ja\nymp\u00e4rist\u00f6yst\u00e4v\u00e4llisyytens\u00e4 takia. Kaikki ketoneja pelkist\u00e4v\u00e4t entsyymit tarvitsevat\ntoimintaansa nikotiiniamidikofaktoria NADH tai NADPH ja voivat yleens\u00e4 katalysoida\nreaktioita sek\u00e4 hapetus- ett\u00e4 pelkistyssuuntaan. Kofaktoreita kierr\u00e4tet\u00e4\u00e4n reaktioissa yleens\u00e4 joko isopropanolia hapettamalla tai erillisell\u00e4 entsyymill\u00e4, kuten glukoosidehydrogenaasilla. Substraatin liukoisuuden parantamiseksi ja konsentraation kasvattamiseksi reaktioissa kannattaa k\u00e4ytt\u00e4\u00e4 veden lis\u00e4ksi jotain orgaanista liuotinta.\n\nKokeellisessa osassa tutkittiin ketoreduktaasien k\u00e4ytt\u00f6\u00e4 valterioni C -nimisen luonnonaineen enantioselektiivisess\u00e4 synteesiss\u00e4. Aineen absoluuttinen konfiguraatio ei ole tiedossa, joten kumpaakin enantiomeeria pit\u00e4isi pysty\u00e4 valmistamaan. Tavoitteena oli selvitt\u00e4\u00e4, miss\u00e4 vaiheessa bentsosuberonista l\u00e4htev\u00e4\u00e4 synteesireitti\u00e4 entsymaattinen pelkistys kannattaa tehd\u00e4. Parhaiten pelkistys onnistuu 8-nitrobentsosuberonilla, koska siit\u00e4 voidaan valmistaa selektiivisesti molempia enantiomeereja ja reaktiot ovat my\u00f6s skaalattavissa. Kumpaakin enantiomeeria valmistettiin noin 15 mg erinomaisella enantioselektiivisyydell\u00e4 (ee >99% ja 97%). Synteesireitin kannalta olisi kuitenkin hyv\u00e4 tehd\u00e4 pelkistys vasta my\u00f6hemmin, vaikka silloin voidaankin valmistaa vain toista enantiomeeria.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Katja K\u00e4rki (kahekark) on 2016-06-02 06:41:01.405199. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-06-02T06:41:02Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201606022819.pdf: 1304254 bytes, checksum: ac527927861e9d5b5801cad68b4a217a (MD5)\nlicense.html: 4805 bytes, checksum: aaf7be928e0992ab8faab37fd8cc79b8 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-06-02T06:41:02Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201606022819.pdf: 1304254 bytes, checksum: ac527927861e9d5b5801cad68b4a217a (MD5)\nlicense.html: 4805 bytes, checksum: aaf7be928e0992ab8faab37fd8cc79b8 (MD5)\n Previous issue date: 2016", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (78 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "ketoreduktaasit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "alkoholidehydrogenaasit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "entsymaattinen pelkistys", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Ketonien stereoselektiivinen pelkistys entsyymeill\u00e4", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201606022819", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Orgaaninen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Organic Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-06-02T06:41:02Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4035", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ketonit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "synteesi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|