| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Tammela, P\u00e4ivi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lepola, Pirkko", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-04-04T09:08:22Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-04-04T09:08:22Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2006", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1525221", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/49243", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Uusia l\u00e4\u00e4kkeit\u00e4 tarvitaan jatkuvasti. Koska luonnonaineet sis\u00e4lt\u00e4v\u00e4t bioaktiivisia yhdisteit\u00e4 ja uusia kemiallisia rakenteita, ne tarjoavat l\u00e4hes rajattoman uusien l\u00e4\u00e4keaineiden l\u00e4hteen. Bioaktiivisuusseulontaan soveltuvan luonnonainekirjaston luomisen avulla voidaan luonnonaineisiin perustuvaa l\u00e4\u00e4kekehitystutkimusta tehostaa lyhent\u00e4en prosessin normaalisti vaatimaa aikaa. T\u00e4ss\u00e4 ty\u00f6ss\u00e4 perustettiin luonnonaine (LA) kirjastoj\u00e4rjestelm\u00e4 L\u00e4\u00e4kkeen keksint\u00e4- ja kehitysteknologian keskukselle (DDTC), joka on monitieteinen tutkimusyksikk\u00f6 Helsingin yliopiston farmasian tiedekunnassa.\r\nKokeellinen LA-kirjasto, joka sis\u00e4lt\u00e4\u00e4 sek\u00e4 n\u00e4ytemateriaalia ett\u00e4 tietokannan, perustettiin kes\u00e4ll\u00e4 2000 satunnaisen lajivalinnan perusteella ker\u00e4tyist\u00e4 40:st\u00e4 suomalaisesta kasvista. Jokainen kasvi oli alun perin joko kokonaisena tai jaoteltuna kahteen, nelj\u00e4\u00e4n tai kuuteen kasvin osaan. Kasvin osista tuotetun kirjaston menetelmin\u00e4 olivat uutto (100% metanoli), kylm\u00e4kuivaus ja mikrofraktiointi k\u00e4ytt\u00e4en korkean erotuskyvyn nestekromatografiaa (HPLC; high-performance liquid chromatography). N\u00e4ytteiden esiprofilointi tehtiin HPLC:lla, k\u00e4ytt\u00e4en k\u00e4\u00e4nteisfaasikolonnia ja gradienttieluutiota (metanoli-vesi, B% 5-95), yhdistettyn\u00e4 diodirividetektoriin ja mittaamalla absorbanssi kahdella aallonpituudella (A: \u03bb 230 nm, B: \u03bb 280 nm). Integrointia jatkossa teht\u00e4viin bioaktiivisuusseulontoihin vahvistettiin luomalla tiedonhallintaj\u00e4rjestelm\u00e4 Microsoft Access XP (2002) relaatiotietokantaan.\r\nTuloksena oli 23 735 n\u00e4ytett\u00e4 sis\u00e4lt\u00e4v\u00e4 4-luokkainen LA-kirjasto (I-IV): (I) Kuivarohdokset, (II) Kuivauutteet, (III) Raakauutteet dimetyylisulfoksidiin (DMSO) liuotettuina, sek\u00e4 (IV) Fraktioidut uutteet. Keskeisimpien n\u00e4ytteiden kokonaislukum\u00e4\u00e4r\u00e4t kirjastossa ovat luokassa I 145 kpl, luokassa II 145 kpl, luokassa III 145 kpl ja luokassa IV 23 300 mikrofraktiota 290:ll\u00e4 96-kuoppaisella n\u00e4ytelevyll\u00e4. LA-kirjaston n\u00e4ytemateriaali s\u00e4ilytet\u00e4\u00e4n joko kuiva-olosuhteissa tai pakastettuna DDTC:n tiloissa, ja kirjaston tiedot ovat sek\u00e4 tietokantatiedostoina (Microsoft Access) ett\u00e4 soveltuvin osin tulostettuina erillisiss\u00e4 DDTC LA-kirjasto \u2013kansioissa. Tietokantaan on tehty varaukset mahdollisille my\u00f6hemmin lis\u00e4tt\u00e4ville luokille, jotka ovat (V) Luonnon puhdasaineet ja (VI) Synteettiset yhdisteet. Kontrolloitu ja helppok\u00e4ytt\u00f6inen LA-kirjasto edist\u00e4\u00e4 sek\u00e4 DDTC:n ett\u00e4 mahdollisten yhteisty\u00f6osapuolten seulontaty\u00f6t\u00e4, tarkoituksena l\u00f6yt\u00e4\u00e4 luonnonaineista bioaktiivisia yhdisteit\u00e4 tai mahdollisten uusien l\u00e4\u00e4keaineiden alkurakenteita nopeammin s\u00e4\u00e4st\u00e4m\u00e4ll\u00e4 kokonaisprosessin aikaa. Microsoft Access tarjoaa k\u00e4yt\u00e4nn\u00f6llisen tietokannan sek\u00e4 tiedon sy\u00f6tt\u00e4miseen, etsint\u00e4\u00e4n ett\u00e4 muokkaamiseen. Kirjastoa voi my\u00f6s laajentaa ja sinne voi lis\u00e4t\u00e4 monen tyyppist\u00e4 lis\u00e4tietoa tarpeen mukaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "New drugs are needed continuously. Natural products offer almost unlimited source of bioactive compounds and novel chemical structures for new drugs. By creating natural product libraries suitable for bioactivity screening, drug discovery from natural products can be intensified with shortened timelines. In this work a natural product (NP) library system was generated for the Drug Discovery and Development Technology Center (DDTC) which is an interdisciplinary research unit belonging to the Faculty of Pharmacy at the University of Helsinki.\r\nThe pilot NP library, including sample material and data base, was generated of 40 dried, randomly selected Finnish plants collected during the summer 2000. The plant samples were originally either whole plants or plants divided into two, four or six parts. Methods to produce the library material of plant parts were extraction (100% methanol), lyophilization and microfractionation by using the high-performance liquid chromatography (HPLC). The primary profiling was done by HPLC, using the reverse-phase column and gradient elution (methanol-water, B% 5-95) coupled with diode-array detection with two wavelengths (A: \u03bb 230 nm, B: \u03bb 280 nm). The integration of the NP library into further bioactivity screening was enhanced by creating a library data management system by using relational database, Microsoft Access XP (2002) software. The coding system was designed on the basis of the library classification and the nature of the samples.\r\nAs a result, the 23 735-membered NP library consists of four classes (I-IV): (I) Crude natural product material, (II) Lyophilized crude natural product extracts, (III) Crude natural product extracts in DMSO, and (IV) Fractionated natural product extracts. Total pivotal quantity of sub-samples (the library material) in Class I was 145, in Class II 145, in Class III 145, and in Class IV 23 300 microfractions in 290 96-well microplates. The NP-library material samples are stored either in dry conditions or in a freezer in the facilities of DDTC, and the library data is in forms of created NP-library data base (Microsoft Access), and as a relevant data in separate DDTC-NP-Library binders. The NP-library database has a reservation for two additional classes (V: Natural compounds and VI: Synthetic compounds) which may be added later on. The controlled and easy-to-operate NP-library enables DDTC, and possible co-operative parties, to perform further screening processes for discovering bioactive compounds or lead structures of potential new drug candidates from natural products more rapidly by shortening the total process time. The Microsoft Access software offers practical platform for both entering, searching and modifying the data. The library can be expanded and several types of additional complex data can be added to the library data base whenever needed.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Miia Hakanen (mihakane) on 2016-04-04 09:08:21.631357. Form: Admin-lomake opinn\u00e4ytteiden julkaisuun (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/admin-lomake-opinnaytteet). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-04-04T09:08:22Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201604041993.pdf: 869134 bytes, checksum: 408e722f4a8cc4e6e27d1ba2b203e74d (MD5)\r\nlicense.html: 107 bytes, checksum: a7d86e598caa500b1b433bbb9dc8ef1c (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-04-04T09:08:22Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201604041993.pdf: 869134 bytes, checksum: 408e722f4a8cc4e6e27d1ba2b203e74d (MD5)\r\nlicense.html: 107 bytes, checksum: a7d86e598caa500b1b433bbb9dc8ef1c (MD5)\r\n Previous issue date: 2006", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (77 s.)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Luonnonaine", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kirjasto", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "l\u00e4\u00e4kekehitystutkimus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "HPLC", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Generation and utilisation of natural product library for bioactivity screening and drug discovery", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201604041993", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-04-04T09:08:22Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "l\u00e4\u00e4kesuunnittelu", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "luonnonaineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suuren erotuskyvyn nestekromatografia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kirjastot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|