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[{"key": "dc.contributor.advisor", "value": "Pawlowski Alice", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Bamford jaana", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Moilanen, Anni", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2016-02-10T06:42:12Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2016-02-10T06:42:12Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2015", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1522096", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/48714", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "VP11 on termofiilisen bakteriofagin P23-77 kapsidiproteiini ja se muodostaa ikosahedraalisen kapsidirakenteen yhdess\u00e4 p\u00e4\u00e4kapsidiproteiinien, virusproteiini (VP)16 ja VP17, kanssa. VP11 tarkkaa paikkaa ja toimintaa kapsidissa ei tiedet\u00e4. Aiemmissa tutkimuksissa VP11 on arveltu sijaitsevan joko kapsidikuoren alapuolella tai kapsidin ulkopinnalla piikkirakenteissa. Tutkimuksen tavoitteena oli optimoida VP11 havaitsemisessa k\u00e4ytett\u00e4v\u00e4 western blot \u2013menetelm\u00e4, arvioida VP11 m\u00e4\u00e4r\u00e4\u00e4 kapsidissa ja tutkia VP11 sijaintia vasta-ainev\u00e4rj\u00e4yksen ja transmissio elektronimikroskopian (TEM) avulla. Western blot \u2013menetelm\u00e4n optimoinnissa k\u00e4ytettiin puoli kuivaa (engl. semi-dry) menetelm\u00e4\u00e4 vaihtelemalla vasta-ainev\u00e4rj\u00e4ysolosuhteita. VP11 m\u00e4\u00e4r\u00e4\u00e4 kapsidissa analysoitiin denaturoivan polyakryyliamidigeelin juovien intensiteetteihin avulla. P23-77 kasvatettiin is\u00e4nt\u00e4bakteeri Thermus thermophiluksessa, jota seurasi kaksivaiheinen viruspuhdistus. Puhdistettu virus vasta-aineleimattiin ja kuvattiin TEMilla. Western blot \u2013menetelm\u00e4n optimointi onnistui, menetelm\u00e4 antoi tarkan signaalin optimaalisten vasta-ainev\u00e4rj\u00e4ysolosuhteiden l\u00f6ydytty\u00e4. Lis\u00e4ksi western blot \u2013tulokset viittasivat siihen ett\u00e4 VP11 on rakenteeltaan dimeeri. VP11 kopiom\u00e4\u00e4r\u00e4n arvioitiin olevan 147, joka viittaa siihen ett\u00e4 VP11 ei ole osallisena viruksen kapsidin piikkirakenteissa. Alustavat TEM kokeet tukevat sit\u00e4 ett\u00e4 VP11 ei sijaitse kapsidin ulkopinnalla. Saatujen tulosten perusteella VP11 sijaitsee viruskapsidin sis\u00e4puolella dimeeri-rakenteena. Proteiinin sijainnin ja muiden ominaisuuksien vuoksi VP11 saattaa olla hy\u00f6dyllinen tulevaisuuden bioalan sovelluksissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "VP11 is a minor capsid protein of the thermophilic bacteriophage P23-77 and comprises the icosahedral capsid mainly with two major capsid proteins (MCPs) virion protein (VP) 16 and VP17. The exact localization and function of VP11 is unknown. Yet, previous studies suggests that VP11 is located either between the protein capsid and the internal lipid membrane or occupies the capsid vertices on the outer side of the capsid. The aims of the study were to optimize a western blot method for the detection of VP11, estimate the copy number of VP11 in the virus particle and study the localization of VP11 by using immunolabelling and transmission electron microscopy (TEM). Western blots were performed with purified virus samples in a semi-dry transfer system with varying immunolabelling conditions. The copy number of VP11 was analyzed based on band intensities in denaturing polyacrylamide gels. P23-77 was cultured by using Thermus thermophilus as a host and purified by rate zonal and equilibrium centrifugation. Purified virus particles were immunolabelled for TEM. The optimization of the western blot method succeeded, specific signals were received after suitable immunolabelling conditions were found. In addition, the western blot results imply that VP11 exists as a dimer in the P23-77 virion. The copy number of VP11 was estimated to be approximately 147 copies. Both findings indicate that VP11 is not the penton protein occupying the capsid vertices. Supporting this theory, preliminary studies with TEM suggest that VP11 does not exist on the outer side of the capsid. Rather, VP11 is located underneath the protein shell as a dimer. Due to its proposed location and other qualities, VP11 might have potential in future biochemical applications.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Anni Moilanen (annmarmo) on 2016-02-10 06:42:12.060113. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2016-02-10T06:42:12Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201602101524.pdf: 1380051 bytes, checksum: 644ffb12591321b490fc2fcd7ce75cfe (MD5)\nlicense.html: 4842 bytes, checksum: 8c3786db104c0ce0f2c6b17446c43a9c (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2016-02-10T06:42:12Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201602101524.pdf: 1380051 bytes, checksum: 644ffb12591321b490fc2fcd7ce75cfe (MD5)\nlicense.html: 4842 bytes, checksum: 8c3786db104c0ce0f2c6b17446c43a9c (MD5)\n Previous issue date: 2015", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (39 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Immunodetection of VP11, the minor capsid protein of thermophilic bacteriophage P23-77", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201602101524", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2016-02-10T06:42:13Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kapsidi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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