| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Toppari, Jussi", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Boxuan, Shen", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Saastamoinen, Janne", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2015-09-01T10:39:06Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2015-09-01T10:39:06Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2015", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1494060", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/46732", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "DNA-origamilla tarkoitetaan j\u00e4rjestelm\u00e4\u00e4, jossa yksijuosteinen DNA-molekyyli on taiteltu tiettyyn ennaltam\u00e4\u00e4r\u00e4ttyyn muotoon. Haluttua muotoa aproksimoidaan liit\u00e4m\u00e4ll\u00e4 yhteen samansuuntaisia DNA-kierteit\u00e4, joiden l\u00e4pi kulkee koko rakenteen matkalta erillinen scaffoldjuoste. T\u00e4m\u00e4 juoste luo lis\u00e4\u00e4 linkkej\u00e4 kierteiden v\u00e4lille ja liitt\u00e4\u00e4 niiden irtonaiset p\u00e4\u00e4t yhteen. Rakennetta koossapit\u00e4vien liitosjuosteiden (staple strands) avulla scaffold-juosteen taittumista pystyt\u00e4\u00e4n ohjaamaan ja luomaan vastakkaissuuntaisista DNA-kierteist\u00e4 koostuva kaksiulotteinen origami. Kolmiulotteinen origami muodostetaan taivuttamalla edell\u00e4mainittua litte\u00e4\u00e4, kaksiulotteista origamia siten, ett\u00e4 se muodostaa itsens\u00e4 kanssa kennomaisen hilarakenteen, jota koossapit\u00e4m\u00e4\u00e4n lis\u00e4t\u00e4\u00e4n kierteiden v\u00e4lille liitosjuosteita. T\u00e4t\u00e4 perusrakennetta muokkaamalla voidaan t\u00e4m\u00e4n j\u00e4lkeen saada aikaan haluttu kolmiulotteinen muoto origamille. Origamien ja muiden DNA-rakenteiden suunnitteluun k\u00e4ytet\u00e4\u00e4n t\u00e4h\u00e4n tarkoitukseen varta vasten tehtyj\u00e4 tietokoneohjelmia, jolloin pystyt\u00e4\u00e4n helposti luomaan oikea sekvenssi juosteille.\n Dielektroforeesissa polarisoituva kappale, joka voi olla joko neutraali tai varattu, liikkuu ep\u00e4homogeenisessa s\u00e4hk\u00f6kent\u00e4ss\u00e4. Kappaleen vastakkaisille pinnoille indusoituu t\u00e4ll\u00f6in s\u00e4hk\u00f6kent\u00e4n vaikutuksesta positiivinen ja negatiivinen varausjakauma, jotka vuorovaikuttavat s\u00e4hk\u00f6kent\u00e4n kanssa ja saavat aikaan Coulombin lain mukaisen voiman. Koska dielektroforeesissa k\u00e4ytett\u00e4v\u00e4 s\u00e4hk\u00f6kentt\u00e4 on ep\u00e4homogeeninen, n\u00e4m\u00e4 voimat ovat erisuuruisia ja siten nettovoima on nollasta poikkeava aiheuttaen kappaleen liikkeen.\n Dielektroforeesia voidaan k\u00e4ytt\u00e4\u00e4 \u201dvangitsemaan\u201d n\u00e4ytteen partikkeleita elektrodien v\u00e4liin. T\u00e4m\u00e4 onnistuu lis\u00e4\u00e4m\u00e4ll\u00e4 elektrodien ymp\u00e4rille n\u00e4ytepartikkeleita sis\u00e4lt\u00e4v\u00e4\u00e4 liuosta ja kytkem\u00e4ll\u00e4 niiden v\u00e4lille s\u00e4hk\u00f6kentt\u00e4. DNA:n tapauksessa k\u00e4ytetyn s\u00e4hk\u00f6kent\u00e4n tulee olla AC-kentt\u00e4, sill\u00e4 molekyylin negatiivisesta varauksesta johtuen kaikki molekyylit liikkuisivat muutoin positiiviselle elektrodille sen sijaan, ett\u00e4 ne sijoittautuisivat keskelle elektrodien v\u00e4lill\u00e4 olevaa aukkoa. Jos n\u00e4ytteen permittiivisyys on sopiva, partikkelit liikkuvat kohti elektrodien v\u00e4liss\u00e4 keskell\u00e4 sijaitsevaa s\u00e4hk\u00f6kent\u00e4n maksimia ja j\u00e4\u00e4v\u00e4t paikalleen. Dielektroforeettisen voiman lis\u00e4ksi molekyylien liikkeeseen n\u00e4yteliuoksessa vaikuttavat sek\u00e4 termiset voimat ett\u00e4 satunnainen Brownin liike, joka\npahimmillaan voi jo itsess\u00e4\u00e4n olla dielektroforeettista voimaa suurempi ja est\u00e4\u00e4 vangitsemisen. T\u00e4st\u00e4 johtuen on erityisen t\u00e4rke\u00e4\u00e4, ett\u00e4 vangittaville partikkeleille Brownin satunnaisvoima on tarpeeksi pieni ja ett\u00e4 k\u00e4ytetty n\u00e4yteliuos on johtavuusominaisuuksiltaan sopiva est\u00e4\u00e4kseen l\u00e4mp\u00f6konvektioiden vaikutukset vangitsemiseen. DNA-rakenteiden tapauksessa dielektroforeettista vangitsemista voidaan hy\u00f6dynt\u00e4\u00e4 esimerkiksi mitattaessa DNA:n s\u00e4hk\u00f6njohtavuutta, sill\u00e4 t\u00e4ll\u00e4 menetelm\u00e4ll\u00e4 voidaan suhteellisen yksinkertaisesti erist\u00e4\u00e4 yksitt\u00e4inen partikkeli tutkittavaksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Janne Saastamoinen (jaerjasa) on 2015-09-01 10:39:06.131920. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2015-09-01T10:39:06Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201509012780.pdf: 2734308 bytes, checksum: 3ff11eb6746fa9fb1fe136b06762e872 (MD5)\nlicense.html: 4810 bytes, checksum: 4f58a0dd0abe45e901a9cd53021f930c (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2015-09-01T10:39:06Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201509012780.pdf: 2734308 bytes, checksum: 3ff11eb6746fa9fb1fe136b06762e872 (MD5)\nlicense.html: 4810 bytes, checksum: 4f58a0dd0abe45e901a9cd53021f930c (MD5)\n Previous issue date: 2015", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (41 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "dielektroforeesi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "DNA", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "DNA-origami", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Dielectrophoretic trapping of 3D-DNA origamis", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201509012780", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Fysiikan laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Physics", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Fysiikka", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Physics", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2015-09-01T10:39:07Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4021", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|