| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Timonen, Jussi", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Aho, Vesa", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Ruhanen, Tuukka", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2015-02-16T18:15:58Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2015-02-16T18:15:58Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2014", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1466422", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/45337", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Rasterikuvakorrelaatiospektroskopia (RICS) on kvantitatiivinen mikroskopiamenetelm\u00e4 molekyylidynamiikan tutkimiseen kaksiulotteisista konfokaalikuvista. Tutkielmassa k\u00e4yd\u00e4\u00e4n l\u00e4pi menetelm\u00e4n teoreettiset ja kokeelliset l\u00e4ht\u00f6kohdat, ja niiden pohjalta m\u00e4\u00e4ritet\u00e4\u00e4n kaupallisen konfokaalimikroskooppij\u00e4rjestelm\u00e4n (Nikon A1R+, Jyv\u00e4skyl\u00e4n yliopiston Nanotiedekeskus) soveltuvuus diffuusion mittaamiseen RICS-menetelm\u00e4ll\u00e4. J\u00e4rjestelm\u00e4n havaittiin soveltuvan heikosti erityisesti nopeiden molekyylien, kuten EGFP-proteiinin mittaamiseen. Suurin haitta oli ilmaisimen h\u00e4iri\u00f6signaali, jonka seurauksena korrelaatioanalyysin tulokset poikkesivat merkitt\u00e4v\u00e4sti kirjallisuusarvoista. Ty\u00f6ss\u00e4 tehtiin lis\u00e4ksi yleisi\u00e4 huomioita RICS-menetelm\u00e4n yksityiskohdista. Esimerkiksi mikroskoopin skannausnopeuden muuttumisella todettiin olevan yll\u00e4tt\u00e4v\u00e4n suuri merkitys ottaen huomioon sen, ettei sit\u00e4 tiett\u00e4v\u00e4sti ole k\u00e4sitelty aiemmissa julkaisuissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Raster image correlation spectroscopy (RICS) is a microscopy technique for quantitatively studying the dynamics of fluorescent species from 2D confocal images. In this thesis the theoretical and practical principles of RICS are discussed and demonstrated. On the basis of the theory, the applicability of a commercial confocal microscope system (Nikon A1R+, Nanoscience Center, University of Jyv\u00e4skyl\u00e4) for RICS diffusion measurements is determined. The system was found to be inefficient especially for measuring fast molecules, such as EGFP. The greatest drawback was high detector correlation, because of which results determined from fast fluctuations deviated significantly from literature values. Measurements were performed also to study the RICS method on a more general level. For example variable scan speed was found to be surprisingly important considering the fact that apparently it has not been discussed in previous publications.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Tuukka Ruhanen (tutaruha) on 2015-02-16 18:15:56.966575. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2015-02-16T18:15:58Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201502161327.pdf: 2746664 bytes, checksum: 12b7c30f31651f844487641e5f97513c (MD5)\nlicense.html: 4840 bytes, checksum: 8e0911de5e10a60f68172fb859e5a61e (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2015-02-16T18:15:58Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201502161327.pdf: 2746664 bytes, checksum: 12b7c30f31651f844487641e5f97513c (MD5)\nlicense.html: 4840 bytes, checksum: 8e0911de5e10a60f68172fb859e5a61e (MD5)\n Previous issue date: 2014", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (51 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "konfokaalimikroskopia", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Rasterikuvakorrelaatiospektroskopiamittauksia Nikon A1R+ -konfokaalimikroskoopilla", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201502161327", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Fysiikan laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Physics", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Fysiikka", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Physics", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2015-02-16T18:15:59Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4021", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikroskopia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fluoresenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fluoresenssimikroskopia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "diffuusio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|