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[{"key": "dc.contributor.advisor", "value": "Vuento, Matti", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Carp\u00e9n, Olli", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Heiska, Leena", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2015-01-19T14:25:56Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2015-01-19T14:25:56Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2014", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/45122", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Tutkimukseni selvitt\u00e4\u00e4 esriinin teht\u00e4vi\u00e4 ja s\u00e4\u00e4tely\u00e4 erityisesti sy\u00f6v\u00e4n kannalta. Esriini kuuluu ERM-proteiiniperheeseen (esriini/radiksiini/moesiini). N\u00e4m\u00e4 proteiinit tarttuvat toisaalta solun aktiinitukirankaan, toisaalta solukalvon reseptoreihin. Esriini vaikuttaa solutukirangan j\u00e4rjest\u00e4ytymiseen ja solun kortikaalisiin muodonmuutoksiin. Voimistunut esriinin ekspressio korreloi tiettyjen sy\u00f6p\u00e4tyyppien maligniteettiin ja erityisesti metastointikykyyn. Esriinin tiedet\u00e4\u00e4n esimerkiksi olevan v\u00e4ltt\u00e4m\u00e4t\u00f6n osteosarkooman metastaasissa. \nTutkimuksen tavoitteena oli selvitt\u00e4\u00e4 Src-kinaasin katalysoiman fosforyloinnin merkityst\u00e4 esriinin toiminnalle sy\u00f6p\u00e4\u00e4n liittyviss\u00e4 ilmi\u00f6iss\u00e4, esim. invaasiossa ja migraatiossa. Tutkimme soluja, jotka eiv\u00e4t ilmenn\u00e4 endogeenist\u00e4 esriini\u00e4 (poistogeenisist\u00e4 hiirist\u00e4 tuotetut solulinjat). Solulinjoissa ilmennettiin esriiniproteiinin erilaisia mutanttiversioita ja solujen k\u00e4ytt\u00e4ytymist\u00e4 verrattiin toisiinsa Src-kinaasiin liittyviss\u00e4 funktionaalisissa kokeissa. Solujen prolife\u00acraatiokyvyn, adheesiokyvyn ja migraatiokyvyn havaittiin olevan kesken\u00e4\u00e4n verrannollisia normaaleissa kaksiulotteisissa viljelyolosuhteissa. Jos soluja kuitenkin kasvatettiin kolmiulotteisten matriksien sis\u00e4ll\u00e4 tai p\u00e4\u00e4ll\u00e4, saatiin mielenkiintoisia tuloksia. T\u00e4llaiset kasvatusolosuhteet muistuttavat fysiologisia kasvuolosuhteita, joita kasvainsolut kudoksissa kohtaavat. Villityyppisen esriinin ilment\u00e4minen antoi soluille selv\u00e4n kasvuedun sek\u00e4 ns. soft agar kokeessa, jossa soluja kasvatetaan kiinte\u00e4n agarin sis\u00e4ll\u00e4, ett\u00e4 Matrigel-kokeissa, joissa solut kasvavat hiiren tuumorista uutetun tyvikalvomatriksin p\u00e4\u00e4ll\u00e4 tai sis\u00e4ll\u00e4. Sit\u00e4 vastoin esriinin Y477F-mutanttimuoto ei kasva n\u00e4iss\u00e4 olosuhteissa esriini\u00e4 ilment\u00e4m\u00e4t\u00f6nt\u00e4 vektorikontrollia paremmin. Villi\u00actyyppisen esriinin antama kasvuetu oli lis\u00e4ksi Src-kinaasista riippuvainen, koska ilman aktiivista Src-kinaasia solut eiv\u00e4t kasvaneet n\u00e4iss\u00e4 oloissa esriinist\u00e4 huolimatta. Samankaltaisia tuloksia saimme kollageenimatriksilla kasvatetuilla solusferoideilla. N\u00e4m\u00e4 tulokset viittaavat siihen, ett\u00e4 esriinin Src-v\u00e4litteisen fosforylaation merkitys liittyy nimenomaan fosforylaation solulle antamaan kykyyn selviyty\u00e4 paremmin kolmiulotteisissa kasvuoloissa, kuten kudoksissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Leena Heiska (lekrheis) on 2015-01-19 14:25:55.607589. Form: Lisensiaatinty\u00f6-lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/lisensiaatintyo-lomake). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2015-01-19T14:25:56Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201501191140.pdf: 1024314 bytes, checksum: a7c131465973a63cfeef8cd728687928 (MD5)\nlicense.html: 4962 bytes, checksum: 05da366a4c27691aa417406bf94a954e (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2015-01-19T14:25:56Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201501191140.pdf: 1024314 bytes, checksum: a7c131465973a63cfeef8cd728687928 (MD5)\nlicense.html: 4962 bytes, checksum: 05da366a4c27691aa417406bf94a954e (MD5)\n Previous issue date: 2014", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (69 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "esriini", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Esriinin merkitys sy\u00f6v\u00e4ss\u00e4", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "licentiate thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201501191140", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Licentiate thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Lisensiaatinty\u00f6", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2015-01-19T14:25:56Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_46ec", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "book", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kasvaimet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "et\u00e4pes\u00e4kkeet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fosforylaatio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G3", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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