| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "M\u00e4ki, Anita", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2014-12-13T18:03:19Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2014-12-13T18:03:19Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2011", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1453741", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/44868", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Integriinien ja kasvutekij\u00e4reseptorien kulku solussa yhdistyy monimutkaisen vuorovaikutusten verkoston kautta. Ymp\u00e4rist\u00f6n olosuhteista riippuvaisesti integriinit ja kasvutekij\u00e4reseptorit kantavat p\u00e4\u00e4asiallisen vastuun solun selviytymisest\u00e4, kasvusta, erilaistumisesta, vaeltamisesta ja lis\u00e4\u00e4ntymisest\u00e4. Integriinit kiinnitt\u00e4v\u00e4t solut alustaansa ja kasvutekij\u00e4reseptorit voivat vastata liukoisten ligandien haasteisiin. \u03b12\u03b21-integriinit reagoivat ligandeihinsa, joita voivat olla matriksimolekyylit, virukset ja keinotekoisesti my\u00f6s vasta-aineet. Integriinien klusterointi viruksella tai vasta-aineilla aiheuttaa integriinin internalisaation endosomeihin, kun taas sitoutuminen matriksimolekyyleihin saa aikaan integriinien kulkeutumisen fokaa-liadheesioihin. Epidermaalisen kasvutekij\u00e4reseptorin (EGFR) aktivaatio on riippuvaista kasvutekij\u00e4ligan-din kiinnittymisest\u00e4 reseptoriin. Integriinien klusteroituminen ja EGFR:n stimulaatio johtaa reseptorien internalisoitumiseen sek\u00e4 aktivoituneeseen signaaliv\u00e4litykseen, jonka t\u00e4ytyy olla tarkasti organisoitua sek\u00e4 ajallisesti ett\u00e4 paikallisesti.\r\nT\u00e4m\u00e4n tutkimuksen tavoitteena oli testata \u03b12\u03b21-integriinin ja stimuloidun EGFR:n vuorovaikutuksia. Tutkimuksessa selvitettiin \u03b12\u03b21-integriinien klusteroinnin vaikutusta EGFR:n polkuun ja EGF-stimulaation merkityst\u00e4 \u03b12\u03b21-integriinin polkuun. Lis\u00e4ksi tutkittiin EGFR:n ja \u03b12\u03b21-integriinin internali-saatiopolkujen vuorovaikutuksia. Tutkimuksessa k\u00e4ytettiin immunoleimaamista, konfokaalimikroskopiaa, l\u00e4p\u00e4isyelektronimikroskopiaa ja el\u00e4vien solun kuvaamista laajakentt\u00e4mikroskoopilla. Tulokset osoittivat, ett\u00e4 integriinien klusterointi hidastaa EGFR:ien hajotusta, mutta EGF-stimulaatio ei vaikuta integriinien hajotukseen. Tutkimuksessa havaittiin solun pinnalla olevaa kolokalisaatiota \u03b12\u03b21-integriinin ja stimu-loimattoman EGFR:n v\u00e4lill\u00e4. Stimulaation ja internalisaation j\u00e4lkeen kolokalisaatiota l\u00f6ydettiin kuitenkin vain v\u00e4h\u00e4n. El\u00e4vien solujen kuvaaminen osoitti enemm\u00e4n kolokalisaatiota perustuen todenn\u00e4k\u00f6isesti kuvausten heikompaan resoluutioon. Tutkimuksessa tarkasteltiin my\u00f6s mahdollisia kolokalisaation virhe-tulkintoja laajakentt\u00e4mikroskopiakuvausten analyyseiss\u00e4. T\u00e4m\u00e4n tutkimuksen tuloksista voidaan p\u00e4\u00e4tell\u00e4, ett\u00e4 integriinien klusterointi toimii negatiivisena s\u00e4\u00e4telij\u00e4n\u00e4 EGFR:ien hajotukselle. Vaikka EGFR ja \u03b12\u03b21-integriinit kolokalisoituvat osittain solun pinnalla, internalisaation j\u00e4lkeen reseptoreja sis\u00e4lt\u00e4v\u00e4t endosomit kulkivat samoja reittej\u00e4 ilman merkitt\u00e4v\u00e4\u00e4 kolokalisaatiota. Huolimatta v\u00e4h\u00e4isest\u00e4 kolokalisaa-tiosta EGFR:n ja integriinin v\u00e4lill\u00e4 sytoplasmisissa rakenteissa, integriinin internalisaatioreitill\u00e4 on selke\u00e4 vaikutus EGFR-reitin toimintaan solussa. EGFR:n v\u00e4hentynyt hajoaminen solussa mahdollisesti lis\u00e4\u00e4 EGFR:n signalointia solussa, pit\u00e4\u00e4 solua pitemp\u00e4\u00e4n hengiss\u00e4 ja voi olla siten edullinen viruksen lis\u00e4\u00e4nty-misen kannalta.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "The pathways of integrins and growth factor receptors are connected through a complex network of inte-ractions in the cell. Depending on the environmental conditions these receptors are largely responsible for the survival, growth, differentiation, migration and proliferation of the cell. Integrins attach cells to the matrix and growth factor receptor enables cells to react to the soluble ligans. \u03b12\u03b21-integrins respond to their ligands i.e. matrix molecules, viruses, and artificially also antibodies. Integrin clustering by viruses or antibodies results in endocytosis of integrins to endosomes whereas binding to the matrix molecules targets integrins to focal adhesions. Epidermal growth factor receptor (EGFR) activation depends on binding of the growth factor ligand. Integrin clustering and EGFR activation result in receptor internaliza-tion and activation of signaling transduction pathways which have to be precisely orchestrated spatially and temporally.\r\nThe aim of this study was to test the interactions between \u03b12\u03b21-integrin and EGFR triggered pathways. The objective of this study was to examine the clustering effects of \u03b12\u03b21-integrin on EGFR pathway and the effects of EGF-stimulation on \u03b12\u03b21-integrin pathway. Furthermore the interactions of the internaliza-tion pathways of EGFR and \u03b12\u03b21-integrin were studied. The testing included immunolabelling experi-ments, confocal fluorescence microscopy, transmission electron microscopy, and live-cell imaging. The results indicated that the clustering of integrins delay the degradation of EGFR but no effect on the deg-radation of integrins was discovered by the EGF-stimulation. In this study colocalization on the cell sur-face was observed between \u03b12\u03b21-integrin and unstimulated EGFR. However, after stimulation and inter-nalization very low levels of colocalization were found. The live cell imaging showed higher colocaliza-tion probably based on poor resolution. The possible misinterpretations of colocalization concerning the analysis of wide-field microscopy were also under consideration. Findings of this study suggest that the clustering of integrins functions as a negative regulator for EGFR degradation. Though EGFR and \u03b12\u03b21-integrin partly colocalize on the cell surface, after internalization, endosomes containing receptors fol-lowed the same routes without considerable colocalization. Regardless of the low colocalization between EGFR and integrins in the cytoplasmic structures, integrin internalization pathway clearly contributes to the EGFR route in the cell. Reduced degradation of EGFR in the cell probably enhances EGFR signal-ing, prolongs the life-time of the cell and may be thus advantageous to the proliferation of virus.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Anita M\u00e4ki (seanruus) on 2014-12-13 18:03:18.370386. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2014-12-13T18:03:18Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201412133497.pdf: 1454750 bytes, checksum: c44c8dbc1dc6975de24a8ffecdc616f5 (MD5)\r\nlicense.html: 4843 bytes, checksum: a5212f618f265e7dedc369d3e8a589d6 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2014-12-13T18:03:19Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201412133497.pdf: 1454750 bytes, checksum: c44c8dbc1dc6975de24a8ffecdc616f5 (MD5)\r\nlicense.html: 4843 bytes, checksum: a5212f618f265e7dedc369d3e8a589d6 (MD5)\r\n Previous issue date: 2011", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (61 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "epidermaalinen kasvutekij\u00e4reseptori", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "\u03b12\u03b21-integriini", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "endosytoosi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "\u03b12\u03b21-integriinin ja stimuloidun epidermaalisen kasvutekij\u00e4-reseptorin reittien yhteydet", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201412133497", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2014-12-13T18:03:19Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "reseptorit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "integriinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endosytoosi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|