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[{"key": "dc.contributor.author", "value": "Marjakangas, Jenni", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2014-10-28T11:12:59Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2014-10-28T11:12:59Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2014", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1447964", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/44496", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "ssDNA-bakteriofageja esiintyy ymp\u00e4rist\u00f6ss\u00e4 huomattavasti enemm\u00e4n, kuin mit\u00e4 on aiemmin oletettu. Viime aikoina kehittyneet virusten laskentametodit ovatkin aiheuttaneet ainoan tunnetun ssDNA-faagiperheen, Microviridae:n, nopean kasvun ja monipuolistumisen. T\u00e4ysin uusia ssDNA-faagiperheit\u00e4 ei ole kuitenkaan l\u00f6ydetty. T\u00e4ss\u00e4 tutkielmassa karakterisoitiin Jyv\u00e4sj\u00e4rvest\u00e4 eristetty\u00e4 uutta ssDNA-bakteriofagi-V155:sta. Tavoitteena oli tutkia kyseisen faagin erityispiirteit\u00e4, sek\u00e4 selvitt\u00e4\u00e4, kuuluuko se Microviridae-perheeseen. Metagenomianalyysien ansiosta ssDNA-faageja tiedet\u00e4\u00e4n esiintyv\u00e4n runsaasti sis\u00e4vesiss\u00e4, mutta t\u00e4m\u00e4 on ensimm\u00e4inen kerta, kun j\u00e4rvest\u00e4 eristetty\u00e4 ssDNA-faagia kasvatetaan laboratorio-olosuhteissa sen morfologian, is\u00e4nt\u00e4kirjon sek\u00e4 elinkierron karakterisoimiseksi. Bakteriofagin genomi todettiin ssDNA:ksi nukleaasik\u00e4sittelyill\u00e4, ja sen proteiiniprofiilia sek\u00e4 mahdollisia lipidirakenteita analysoitiin SDS-PAGE:lla. V155:n ja sen is\u00e4ntien morfologiaa sek\u00e4 faagi-is\u00e4nt\u00e4-vuorovaikutuksia tarkasteltiin TEM-kuvantamisen avulla. Lis\u00e4ksi faagin is\u00e4nt\u00e4kirjoa sek\u00e4 elinkiertoa tutkittiin infektiokokeilla sek\u00e4 plakkianalyysill\u00e4. Bakteriofagin kapsidin havaittiin olevan ikosahedraalinen tunnettujen Microviridae-faagien tapaan, mutta TEM:n mukaan sen koko eroaa huomattavasti muiden Microviridae-faagien kapsideista. Kloroformitestin ja lipidiv\u00e4rj\u00e4yksen perusteella V155:ss\u00e4 n\u00e4ytt\u00e4isi olevan lipidej\u00e4, mutta t\u00e4m\u00e4 t\u00e4ytyy viel\u00e4 vahvistaa my\u00f6s muilla kokeilla. Kalvorakenteiden l\u00f6ytyminen tekisi tutkitusta faagista ainutlaatuisen tunnettujen ssDNA-faagien joukossa. Is\u00e4nt\u00e4kirjotestiss\u00e4 faagi kykeni infektoimaan kolmea eri Flavobacterium-kantaa. T\u00e4m\u00e4 on ensimm\u00e4inen kerta, kun ssDNA-faagin on havaittu infektoivan kyseist\u00e4 bakteerisukua. Lis\u00e4ksi V155:n muodostamat plakit sek\u00e4 ongelmat nestekasvatuksessa viittaavat faagin kykyyn siirty\u00e4 tarvittaessa lysogeeniseen elinkiertoon, mik\u00e4 on melko harvinaista ssDNA-faagien keskuudessa. V155 osoittaa siis selvi\u00e4 samankaltaisuuksia Microviridae-perheeseen, mutta l\u00f6ydetyt eroavaisuudet viittaavat kuitenkin siihen, ett\u00e4 tutkittu bakteriofagi kuuluu mit\u00e4 todenn\u00e4k\u00f6isimmin uuteen Microviridae-alaperheeseen, ellei jopa t\u00e4ysin uuteen virusperheeseen. V155:n rakenne- sek\u00e4 sekvenssitutkimusta tarvitaan viel\u00e4 lis\u00e4\u00e4 faagin fylogenian selvitt\u00e4miseksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Recently it has been revealed that the number of ssDNA-phages in the environment is much greater than previously expected. Improved viral counting methods have increased ssDNA-phage detections, resulting in the expansion of the only known ssDNA-phage family Microviridae. However, completely new ssDNA-phage families have not been found. In this study a new ssDNA-bacteriophage V155, previously isolated from the lake Jyv\u00e4sj\u00e4rvi, was characterized. The aim was to study the features of the phage and also find out if V155 belongs to the Microviridae-family. Numerous metagenomic analyses have revealed that ssDNA-phages are common in fresh waters. However, this is the first time that lake derived ssDNA-phage is cultivated in laboratory in order to characterize its morphology, host range and life cycle. The genome of the bacteriophage was confirmed to be ssDNA by various nuclease treatments, and its protein profile and the existence of lipid structures were analyzed in SDS-PAGE. The morphology of the phage and its hosts as well as the phage-host-interactions were researched using electron microscopy. In addition, the host range and the life cycle of the phage were studied using infection experiments and plaque analysis. It was revealed that the capsid of V155 is icosahedral in the same manner as in the other Microviridae. However, the capsid size greatly exceeds that of the known ssDNA-phages. According to the chloroform tests and lipid staining of the electrophoresis gel, the virion seems to contain lipids, yet further research is needed in order to confirm this. If membrane structures were found, it would make V155 unique within the known ssDNA-phages. In the host range experiments it was discovered that the phage is able to infect three different Flavobacterium strains. There are no other reports of Flavobacterium as a host species for Microviridae. Furthermore, the plaque morphology and the difficulties growing the phage in liquid culture indicate, that when needed, V155 is able to shift from lytic to lysogenic life cycle. This is relatively rare within the known ssDNA-phages. According to these results V155 has clear similarities to the Microviridae-family. Nevertheless, the differences revealed during the research make it plausible that this bacteriophage belongs to a new Microviridae-subfamily, or possibly even to a completely new viral family. More structure and sequence research is still needed in order to characterize the phylogeny of V155 comprehensively.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Jenni Marjakangas (jemamarj) on 2014-10-28 11:12:58.500485. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2014-10-28T11:12:59Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201410283115.pdf: 1250681 bytes, checksum: 23cec9a0058f22157eb0fb53aa107cf3 (MD5)\nlicense.html: 4835 bytes, checksum: 5be5b493a45424a2216955dc15d8cf1e (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2014-10-28T11:12:59Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201410283115.pdf: 1250681 bytes, checksum: 23cec9a0058f22157eb0fb53aa107cf3 (MD5)\nlicense.html: 4835 bytes, checksum: 5be5b493a45424a2216955dc15d8cf1e (MD5)\n Previous issue date: 2014", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (48 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "ssDNA-bakteriofagi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "karakterisointi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Microviridae", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Flavobakteeri", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Flavobakteereita infektoivan ssDNA-bakteriofagi V155:n karakterisointi", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201410283115", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2014-10-28T11:12:59Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "perim\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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