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[{"key": "dc.contributor.author", "value": "M\u00e4kinen, Anne", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2014-10-09T10:29:00Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2014-10-09T10:29:00Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2014", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1446888", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/44397", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Anne M\u00e4kinen (2014). Liikunnan ja hera- tai proteiinijuomadieettien vaikutukset sirtuiinien 1-7 ilmentymiseen LCR -rottien raajalihaksessa. Liikuntabiologian laitos, Jyv\u00e4skyl\u00e4n yliopisto, Liikuntafysiologian pro gradu-tutkielma, 41 s. \n\nHistonit ja muut kromosomaaliset proteiinit pakkautuvat DNA:n (deoksiribonukleiinihapon) tumaan. Histonideasetylaasit kontrolloivat osaltaan geenien ilmentymist\u00e4. Ryhm\u00e4n III histonideasetylaaseja nimitet\u00e4\u00e4n sirtuiineiksi, joita on seitsem\u00e4n kappaletta ja niiden toiminta on riippuvainen NAD+:sta (nikotiinihappoamidiadeniinidinukleotidi). Tutkimuksissa on todettu liikunnan lis\u00e4\u00e4v\u00e4n joidenkin sirtuiinien ilmentymist\u00e4, mutta proteiinin tai heran vaikutuksia sirtuiinien ilmentymiseen ei ole tutkittu. T\u00e4ss\u00e4 tutkimuksessa tutkittiin miten hera- ja proteiinijuomadieetit sek\u00e4 liikunta vaikuttavat sirtuiinien ilmentymiseen rottien raajalihak-sessa. \nTutkimuksessa oli 47 Yhdysvalloista hankittua LCR -kannan (low capasity runner) rottaa, joilla metabolisen oireyhtym\u00e4n riskitekij\u00e4t olivat koholla. Rotat jaettiin kuuteen ryhm\u00e4\u00e4n; kontrolliryhm\u00e4 K, heraryhm\u00e4 H, proteiinijuomaryhm\u00e4 P ja n\u00e4iden juoksijakontrollit eli juoksupy\u00f6r\u00e4ryhm\u00e4t K+J, H+J sek\u00e4 P+J. Juoksuintervention aikana rotilla oli ravintoa rajattomasti ja juomana dieettiens\u00e4 mukaisesti joko vesi, hera- tai proteiinijuoma. Interventio kesti 23 viikkoa, jonka j\u00e4lkeen rotat lopetettiin ja kudokset ker\u00e4ttiin talteen. Proteiinim\u00e4\u00e4ritykset tehtiin plantaris -lihaksesta Western Blotting -menetelm\u00e4ll\u00e4. \nSirtuiinien 1 ja 2 ilmentymisess\u00e4 ei ollut tilastollisia merkitsevyyksi\u00e4. Sirtuiini 3 osalta kaikki juoksijaryhm\u00e4t (K+J: p=0.05; P+J: p<0,001 ja H+J: p<0,001) erosivat merkitsev\u00e4sti kontrolliryhmist\u00e4\u00e4n K, P ja H. Liikunta lis\u00e4si sirtuiini 4:n ilmentymist\u00e4 eritt\u00e4in merkitt\u00e4v\u00e4sti kontrolliryhm\u00e4ss\u00e4 K (p<0,001) sek\u00e4 heran juoksijaryhm\u00e4ss\u00e4 H+J (p<0,01). Liikunta yksin ei nostanut sirtuiini 5:n ilmentymist\u00e4, sit\u00e4 vastoin kaikissa muissa ryhmiss\u00e4 tapahtui merkitsev\u00e4\u00e4 (p<0,001) ilmentymisen nousua. Sirtuiini 6: n ilmentymisess\u00e4 K erosi merkitsev\u00e4sti ryhm\u00e4st\u00e4 K+J, P ja H+J (p<0,01; p<0,001; p<0,05). Sirtuiini 7:n ilmentymiseen ei liikunnalla ollut vaikutusta. Merkitsevi\u00e4 eroja oli P+J, H sek\u00e4 H+J ryhmiss\u00e4 kontrolliin K verrattuna (p<0,001). T\u00e4m\u00e4 tutkimus osoitti, ett\u00e4 liikunnalla oli voimakkaita vaikutuksia erityisesti mitokondriaalisten sirtuiinien ilmentymiseen ja my\u00f6s dieetit vaikuttivat sirtuiinien ilmentymiseen. \n\nAvainsanat: Histonideasetylaasit, proteiinit, sirtuiinit", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Anne M\u00e4kinen (annelima) on 2014-10-09 10:29:00.304838. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2014-10-09T10:29:00Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201410092960.pdf: 598620 bytes, checksum: f43dddf6e50fa396708e105b80b51be5 (MD5)\nlicense.html: 4870 bytes, checksum: 23b154dc1c73e60e5566ea9a9c567edd (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2014-10-09T10:29:00Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201410092960.pdf: 598620 bytes, checksum: f43dddf6e50fa396708e105b80b51be5 (MD5)\nlicense.html: 4870 bytes, checksum: 23b154dc1c73e60e5566ea9a9c567edd (MD5)\n Previous issue date: 2014", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto.", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "histonideasetylaasit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sirtuiinit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Liikunnan ja hera- tai proteiinijuomadieetin vaikutukset sirtuiinien 1-7 ilmentymiseen LCR-rottien raajalihaksessa", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201410092960", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikuntabiologian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biology of Physical Activity", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntafysiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Exercise Physiology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2014-10-09T10:29:01Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "5011", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "nukleotidit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "liikunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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