Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis

Triangular shaped particles are an interesting research topic since there are three plasmonically active tips, which can be utilized in new molecular sensing application systems. In this research we used dielectrophoretic force to trap thiol-modified (5’- end) and Cy3-dye-labeled (3’-end) single-...

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Main Author: Andrejeff, Tanja
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Fysiikan laitos, University of Jyväskylä, Jyväskylän yliopisto
Format: Master's thesis
Language:eng
Published: 2014
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/43727
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author Andrejeff, Tanja
author2 Matemaattis-luonnontieteellinen tiedekunta Fysiikan laitos University of Jyväskylä Jyväskylän yliopisto
author_facet Andrejeff, Tanja Matemaattis-luonnontieteellinen tiedekunta Fysiikan laitos University of Jyväskylä Jyväskylän yliopisto Andrejeff, Tanja Matemaattis-luonnontieteellinen tiedekunta Fysiikan laitos University of Jyväskylä Jyväskylän yliopisto
author_sort Andrejeff, Tanja
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description Triangular shaped particles are an interesting research topic since there are three plasmonically active tips, which can be utilized in new molecular sensing application systems. In this research we used dielectrophoretic force to trap thiol-modified (5’- end) and Cy3-dye-labeled (3’-end) single-stranded DNA (ssDNA) oligonucleotides, size 40 nt (about 22 nm long), to the corners of the gold triangles. These gold triangles were 20 nm thick and side length was 1 μm. The trapping experiment was done under AC-circuit and the gathering of the oligos to the triangles was studied in situ under confocal microscope. The theoretical values for dielectrophoretic force with different voltages were simulated with finite element method. Experimentally the required DEP-force was achieved by using about 40 Vpp voltage and in the simulation system with 34 Vpp. Most of the oligos formed “cloud” around the triangle and few of them trapped also on the corners. This “cloud” can be seen also in simulations, where the gathering of the oligos was modeled. At least, our research prove that gold triangles can be used for trapping biomolecules and hence also to sensing and detecting them.
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In this research we used dielectrophoretic force to trap thiol-modified (5\u2019-\r\nend) and Cy3-dye-labeled (3\u2019-end) single-stranded DNA (ssDNA) oligonucleotides,\r\nsize 40 nt (about 22 nm long), to the corners of the gold triangles. These gold\r\ntriangles were 20 nm thick and side length was 1 \u03bcm. The trapping experiment was\r\ndone under AC-circuit and the gathering of the oligos to the triangles was studied\r\nin situ under confocal microscope. The theoretical values for dielectrophoretic force\r\nwith different voltages were simulated with finite element method. Experimentally\r\nthe required DEP-force was achieved by using about 40 Vpp voltage and in the\r\nsimulation system with 34 Vpp. Most of the oligos formed \u201ccloud\u201d around the triangle\r\nand few of them trapped also on the corners. This \u201ccloud\u201d can be seen also in\r\nsimulations, where the gathering of the oligos was modeled. 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spellingShingle Andrejeff, Tanja Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis DEP force fluorescent gold oligo trapping triangle 4033 oligonukleotidit nanotieteet nanotekniikka kulta
title Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_full Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_fullStr Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_full_unstemmed Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_short Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_sort selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
title_txtP Selective trapping of oligos to triangular gold nanoparticles utilizing dielectrophoresis
topic DEP force fluorescent gold oligo trapping triangle 4033 oligonukleotidit nanotieteet nanotekniikka kulta
topic_facet 4033 DEP force fluorescent gold kulta nanotekniikka nanotieteet oligo oligonukleotidit trapping triangle
url https://jyx.jyu.fi/handle/123456789/43727 http://www.urn.fi/URN:NBN:fi:jyu-201406162041
work_keys_str_mv AT andrejefftanja selectivetrappingofoligostotriangulargoldnanoparticlesutilizingdielectrophoresis