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[{"key": "dc.contributor.author", "value": "Lehtilahti, Elisa", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2012-08-19T12:51:28Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2012-08-19T12:51:28Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2012", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1224212", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/38301", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "On yleisesti tiedossa, ett\u00e4 maito on terveellist\u00e4 ja parantaa luun kest\u00e4vyytt\u00e4. Tutkimusten perusteella osa maidon proteiineista lis\u00e4\u00e4 luuta muodostavien osteoblastien m\u00e4\u00e4r\u00e4\u00e4 ja ehk\u00e4isee luun haurastumista est\u00e4m\u00e4ll\u00e4 esiosteoklastien erilaistumista luuta hajottaviksi osteoklasteiksi. Osteoblastit erilaistuvat multipotenteista mesenkymaalisista kantasoluista. T\u00e4ll\u00f6in maidon proteiineilla saattaa olla fysiologinen rooli luun kasvamisessa ja paranemisessa. Maidon proteiineilla voi olla my\u00f6s terapeuttinen vaikutus osteoporoosin anabolisena tekij\u00e4n\u00e4.\nTutkimus oli osa Valion ja Turun yliopiston yhteisty\u00f6projektia. Tutkimuksen tarkoituksena oli testata maidon seitsem\u00e4n proteiinifraktion vaikutuksia mesenkymaalisten kantasolujen luusoluerilaistumiseen. Tarkoituksena oli l\u00f6yt\u00e4\u00e4 maidon proteiineja, jotka edist\u00e4v\u00e4t luun vahvistumista ja luusolujen kasvua. Kuntotestien avulla haluttiin selvitt\u00e4\u00e4 maidon proteiinifraktioiden vaikutuksia kantasolujen kuntoon. Kuntotestej\u00e4 tehtiin 13:lle maidon proteiinifraktiolle. \nP4:n, V30 l\u00e4mp\u00f6k\u00e4sitellyn ja ei-l\u00e4mp\u00f6k\u00e4sitellyn (V30 l\u00e4mp\u00f6 ja ei-l\u00e4mp\u00f6), V50 l\u00e4mp\u00f6k\u00e4sitellyn ja ei-l\u00e4mp\u00f6k\u00e4sitellyn (V50 l\u00e4mp\u00f6 ja ei-l\u00e4mp\u00f6), Valion kaseiiniton kaseiinifraktion (WPC) ja Valion natiivi heraproteiinifraktion (VNH) vaikutuksia tutkittiin mesenkymaalisten kantasolujen luusoluerilaistumiseen. Alkalisen fosfataasin spesifinen aktiivisuus ja muodostuneen kalsiumin m\u00e4\u00e4r\u00e4\u00e4 mitattiin luusoluerilaistumiskasvatuksista. Maidon proteiinien vaikutuksia mesenkymaalisiin kantasoluihin tutkittiin jo aiemmin ryhm\u00e4ss\u00e4mme optimoidulla kuntotestill\u00e4. Edell\u00e4 mainittujen proteiinifraktioiden lis\u00e4ksi kuntotesteihin otettiin mukaan \u03b1-laktalbumiini, \u03b2-laktoglobuliini, laktoferriini, heran kasvutekij\u00e4t (WGF), GMP ja natiivi heraproteiiniproteiinifraktio (NWP). Kuntotestiss\u00e4 k\u00e4ytettiin 5,5\u00b4,6,6\u00b4-tetrakloori-1,1\u00b4,3,3\u00b4- tetraetyylibenzimidazolihiilisyanidi jodia (JC-1), joka aggrekoituu hyv\u00e4kuntoisten kantasolujen mitokondrioon. Mitokondrion depolarisaation aikana JC-1 vapautuu solulimaan ja muodostaa monomeerej\u00e4. JC-1 aggrekaatit ja monomeerit emittoivat eri aallonpituuksilla jolloin tulokset mitattiin virtaussytometrill\u00e4. Tulokset analysoitiin FlowJo \u2013 ohjelmalla. \nKuntotestien perusteella maidon \u03b1-laktalbumiini (p=0.01), \u03b2-laktoglobuliini (p=0.001), P4\u2212 (p=0.01), V30 l\u00e4mp\u00f6\u2212 (p=0.05), V50 l\u00e4mp\u00f6\u2212 (p=0.01) ja ei-l\u00e4mp\u00f6\u2212 (p=0.001), VNH\u2212 (p=0.001), WGF\u2212 (p=0.001) ja GMP\u2212 (p=0.05) proteiinifraktiot paransivat mesenkymaalisten kantasolujen kuntoa. Laktoferriinill\u00e4, V30 ei-l\u00e4mp\u00f6\u2212, WPC\u2212, ja NWP\u2212 proteiinifraktioilla ei ollut vaikutusta mesenkymaalisten kantasolujen kuntoon. Luusoluerilaistumistestien perusteella VNH\u2212 proteiinifraktio n\u00e4ytt\u00e4isi lis\u00e4\u00e4v\u00e4n mesenkymaalisten kantasolujen luusoluerilaistumista suurimmilla pitoisuuksilla ja V50 ei-l\u00e4mp\u00f6\u2212 proteiinifraktio n\u00e4ytt\u00e4isi est\u00e4v\u00e4n mesenkymaalisten kantasolujen luusoluerilaistumista. \nOsa maidon proteiinifraktioista n\u00e4ytt\u00e4isi parantavan mesenkymaalisten kantasolujen kuntoa jo valmiiksi hyv\u00e4kuntoisilla soluilla. Voi olla mahdollista, ett\u00e4 maidon proteiinifraktiot voivat my\u00f6s parantaa huonokuntoisten solujen elinkykyisyytt\u00e4. Luusoluerilaistumistestien tulokset olivat osittain ristiriitaisia ja lopullisten johtop\u00e4\u00e4t\u00f6ksien tekemiseen tarvitaan lis\u00e4\u00e4 m\u00e4\u00e4rityksi\u00e4.", "language": null, "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Elisa Lehtilahti (elkaleht) on 2012-08-19 12:51:28.239781. Form: Pro gradu -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2012-08-19T12:51:28Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201208192170.pdf: 674806 bytes, checksum: e32a0b83bc19c3f81cafe255d6fe420e (MD5)\nlicense.html: 5067 bytes, checksum: 4abe976572086cea4246975863232d88 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2012-08-19T12:51:28Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201208192170.pdf: 674806 bytes, checksum: e32a0b83bc19c3f81cafe255d6fe420e (MD5)\nlicense.html: 5067 bytes, checksum: 4abe976572086cea4246975863232d88 (MD5)\n Previous issue date: 2012", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "72 sivua", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "heraproteiinit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": 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