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[{"key": "dc.contributor.author", "value": "Flink, Riina", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2012-05-28T18:13:58Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2012-05-28T18:13:58Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2010", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/37914", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Riina Flink. 2010. MARP \u2013 proteiiniperheen proteiinien mRNA \u2013 m\u00e4\u00e4r\u00e4t venymis \u2013 lyhenemissykli \u2013 tyyppisen \u2013 kuormituksen j\u00e4lkeen. Kandidaatin tutkielma, Liikuntafysiologia. Liikuntabiologian\r\nlaitos, Jyv\u00e4skyl\u00e4n yliopisto. 37s.\r\n\r\nMARP (muscle ankyrin repeat protein) \u2013 proteiiniperheen proteiinien arvellaan olevan mukana etenkin luurankolihaksen adaptoitumisessa paljon eksentrist\u00e4 lihasliikett\u00e4 sis\u00e4lt\u00e4v\u00e4\u00e4n harjoitukseen. Arvellaan, ett\u00e4 ne saattavat ehk\u00e4ist\u00e4 muun muassa soluvaurioilta. Niiden ilmenemist\u00e4 fyysisen rasituksen yhteydess\u00e4 on kuitenkin tutkittu ihmisill\u00e4 vasta yhdess\u00e4 tutkimuksessa (Lehti ym .2009) ja tietoa tarvitaan lis\u00e4\u00e4. T\u00e4m\u00e4n tutkimuksen tarkoituksena olikin tutkia, miten CARP:in (cardiac ankyrin repeat protein), Ankrd2:n\r\n(ankyrin repeat protein 2) ja DARP:in (diabetes ankyrin repeat protein) mRNA (messenger RNA) - m\u00e4\u00e4r\u00e4t muuttuvat SSC (stretch- shortening- cycle) - kuormituksen seurauksena. M\u00e4\u00e4rityksess\u00e4 k\u00e4ytettiin RTPCR (Real \u2013Time polymerase chain reaction) \u2013 tekniikkaa. Yhdeks\u00e4n koehenkil\u00f6\u00e4 suorittivat saman protokollan,\r\njossa he tekiv\u00e4t yhden jalan hyppyj\u00e4 siihen tarkoitetussa kelkassa uupumukseen saakka, mutta maksimissaan 20 minuuttia. Kontrollibiopsia otettiin v\u00e4sytt\u00e4m\u00e4tt\u00f6m\u00e4n jalan Vastus Lateraliksesta ennen kuormitusta. V\u00e4sytetyn jalan Vastus Lateraliksesta otettiin biopsiat heti kuormituksen j\u00e4lkeen ja 3 tuntia kuormituksesta. CARP:in mRNA - m\u00e4\u00e4r\u00e4 nousi heti kuormituksen j\u00e4lkeen (1740 % \u00b1 4323 % kontrolliarvosta, P=0,01) kolmen tunnin j\u00e4lkeisen m\u00e4\u00e4r\u00e4n ollessa kuitenkin tilastollisesti merkitsev\u00e4sti viel\u00e4 t\u00e4t\u00e4kin suurempi (10184 % \u00b1 11676 % kontrolliarvosta, P= 0,01). Ankrd2:n mRNA m\u00e4\u00e4riss\u00e4 ei havaittu tilastollisesti\r\nmerkitsevi\u00e4 muutoksia, mutta keskiarvoisesti konsentraatio oli kuitenkin huomattavasti noussut kolmen tunnin j\u00e4lkeen kuormituksesta (164 % \u00b1 147 % kontrolliarvosta). DARP:in mRNA - m\u00e4\u00e4riss\u00e4 ei tapahtunut huomattavia muutoksia. N\u00e4m\u00e4 tulokset ovat samansuuntaisia kuin edellisen samankaltaisen tutkimuksen tulokset (Lehti ym. 2009). Tulosten perusteella voidaan p\u00e4\u00e4tell\u00e4, ett\u00e4 CARP:in vaste kuormitukseen on hyvin nopea ja voimakas. Lis\u00e4ksi Ankrd2:n tulokset viittaavat siihen, ett\u00e4 sen vaste ei ole niin\r\nnopea, mutta sen m\u00e4\u00e4r\u00e4 olisi voinut ehk\u00e4 nousta kolmen tunnin j\u00e4lkeen kuormituksesta. Kummankin proteiinin kohdalla keskihajonnat olivat kuitenkin suuria ja esimerkiksi CARP:in kohdalla jopa viiden koehenkil\u00f6n heti kuormituksen j\u00e4lkeinen arvo oli kontrolliarvoa pienempi. Niinp\u00e4 vaihtelut ovat hyvin suuria\r\nja lis\u00e4tutkimuksia tulisi tehd\u00e4 isommalla koehenkil\u00f6m\u00e4\u00e4r\u00e4ll\u00e4. Lis\u00e4ksi MARP:eja tulisi tutkia my\u00f6s proteiinitasolla, sill\u00e4 mRNA ja proteiinitaso eiv\u00e4t aina vastaa toisiaan.", "language": "", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Riina Flink (rielmafl) on 2012-05-28 18:13:58.494084. Form: Kandin tutkielma -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/kandin-tutkielma-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2012-05-28T18:13:58Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201205281748.pdf: 330308 bytes, checksum: 8187fd0acc91dd6c698793187bbcc56f (MD5)\r\nlicense.html: 5044 bytes, checksum: 29cb24006ac12ab83cf9cf4253bcf759 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2012-05-28T18:13:58Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201205281748.pdf: 330308 bytes, checksum: 8187fd0acc91dd6c698793187bbcc56f (MD5)\r\nlicense.html: 5044 bytes, checksum: 29cb24006ac12ab83cf9cf4253bcf759 (MD5)\r\n Previous issue date: 2010", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "35", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "MARP", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "CARP", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Ankrd2", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "DARP", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "PCR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "biopsia", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mRNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lihas", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "MARP - proteiiniperheen proteiinien mRNA \u2013m\u00e4\u00e4r\u00e4t venymis - lyhenemissykli -tyyppisen kuormituksen j\u00e4lkeen", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "bachelor thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201205281748", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Bachelor's thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Kandidaatintutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikuntabiologian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntafysiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2012-05-28T18:13:59Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_7a1f", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "bachelorThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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