| fullrecord |
[{"key": "dc.contributor.author", "value": "Luukkonen, Milja", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2011-06-15T12:53:20Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2011-06-15T12:53:20Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2011", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1162676", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/27185", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Avidiini on tetrameerinen proteiini, jota on tutkittu ja k\u00e4ytetty paljon ligandien sitomiskykyns\u00e4 ansiosta. Avidiinin luonnollinen ligandi on biotiini, jonka affiniteetti avidiiniin on eritt\u00e4in voimakas (Kd=10-15 M). Avidiini kykenee sitomaan my\u00f6s muita ligandeja, mutta pienemm\u00e4ll\u00e4 affiniteetilla. Yksi avidiiniin sitoutuvista molekyyleist\u00e4 on HABA (4\u2019-hydroksiatsobentseeni-2-karboksyylihappo) -atsov\u00e4ri, jota on k\u00e4ytetty avidiini-biotiiniteknologiassa hyv\u00e4ksi. \n\nHABA-molekyylill\u00e4 on kaksi eri rakenteellista isomeerimuotoa, trans- ja cis-isomeerit. Yleens\u00e4 se esiintyy tasomaisena molekyylin\u00e4 eli trans-isomeerina, mutta se voi menett\u00e4\u00e4 tasomaisen rakenteensa ja ilmenty\u00e4 t\u00e4ll\u00f6in cis-isomeerin\u00e4. Avidiiniin sitoutuessaan HABA:n v\u00e4ri muuttuu keltaisesta punertavaksi, ja sitoutuminen voidaan havaita spektroskooppisilla menetelmill\u00e4 absorption muutoksena. Lis\u00e4ksi useita erilaisia HABA:n johdannaisia on syntesoitu, ja ne sitoutuvat avidiiniin eri affiniteetilla. \n\nT\u00e4ss\u00e4 ty\u00f6ss\u00e4 m\u00e4\u00e4ritettiin atsov\u00e4rimolekyyli HABA:n ja avidiini-HABA -yhdisteiden ominaisuuksia spektro-skooppisilla, kromatografisilla ja kalorimetrisilla menetelmill\u00e4. Lis\u00e4ksi HABA-molekyylin ja yhden sen joh-dannaisen (TAM-017) sitoutumista avidiiniin tutkittiin eri olosuhteissa, kuten eri liuottimissa ja pH-arvoissa. N\u00e4iden molekyylien sitoutumista avidiiniin sek\u00e4 HABA-molekyylin konformaatiota yritettiin my\u00f6s kontrol-loida valon ja liuoksen pH:n avulla. T\u00e4ll\u00f6in pH:n havaittiin vaikuttavan HABA:n ja avidiinin v\u00e4liseen vuorovaikutukseen. Mik\u00e4li HABA-yhdisteiden konformaatiota ja sitoutumista avidiiniin voidaan kontrolloida jonkun ulkoisen tekij\u00e4n avulla, HABA-tyyppisi\u00e4 molekyylej\u00e4 voitaisiin hy\u00f6dynt\u00e4\u00e4 laajalti eri tieteenaloilla. T\u00e4m\u00e4n tutkimuksen tulosten perusteella pH n\u00e4ytt\u00e4isikin olevan k\u00e4ytt\u00f6kelpoinen parametri HABA:n sitoutumisen kontrolloimiseksi.", "language": null, "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Milja Luukkonen (miluukko) on 2011-06-15 12:53:18.715248. Form: Pro gradu -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2011-06-15T12:53:20Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-2011061511018.pdf: 1509487 bytes, checksum: 580d536de3c6549708268dd32816b012 (MD5)\nlicense.html: 4997 bytes, checksum: fd61e7f4ece5069c9b29d7457cd89351 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2011-06-15T12:53:20Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-2011061511018.pdf: 1509487 bytes, checksum: 580d536de3c6549708268dd32816b012 (MD5)\nlicense.html: 4997 bytes, checksum: fd61e7f4ece5069c9b29d7457cd89351 (MD5)\n Previous issue date: 2011", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "77 sivua", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "atsobentseeni", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Avidiini-HABA-kompleksin karakterisointi ja kontrollointi", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-2011061511018", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "avidiini", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "isomeria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kontrolli", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|