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[{"key": "dc.contributor.author", "value": "Lampinen, Hanna", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2011-01-25T13:30:58Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2011-01-25T13:30:58Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2010", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1147279", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/25943", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Diapaussi on fysiologinen lepotila, jonka aikana eli\u00f6n elintoiminnot, kasvu ja kehitys hidastuvat ja \u00e4\u00e4riolosuhteiden, kuten alhaisten l\u00e4mp\u00f6tilojen ja kuivuuden, sietokyky paranee. Lis\u00e4\u00e4ntymislepokaudella tarkoitetaan aikuisilla hy\u00f6nteisill\u00e4 esiintyv\u00e4\u00e4 diapaussin muotoa, jonka aikana lis\u00e4\u00e4ntymiseen liittyv\u00e4t toiminnot hidastuvat tai pys\u00e4htyv\u00e4t kokonaan. Drosophila melanogaster -lajin lis\u00e4\u00e4ntymislepokauteen on liitetty useita kandidaattigeenej\u00e4, joista yksi on couch potato (cpo), ja t\u00e4m\u00e4n geenin yhteydest\u00e4 lis\u00e4\u00e4ntymislepokauteen on saatu viitteit\u00e4 my\u00f6s pohjoisella D. montana -lajilla. Pro gradu -ty\u00f6ss\u00e4ni tarkasteltiin cpo-geeniss\u00e4 esiintyv\u00e4n 19 em\u00e4sparin kokoisen deleetiomutaation yleisyytt\u00e4 D. montana -lajin Oulangan luonnonpopulaatiossa ja esiintymist\u00e4 vuonna 2003 ker\u00e4tyn naaraan j\u00e4lkel\u00e4isist\u00e4 koostuvassa laboratoriolinjassa. Kvantitatiivisen PCR:n (polymerase chain reaction) avulla pyrittiin selvitt\u00e4m\u00e4\u00e4n, onko deleetiolla vaikutusta cpo-geenin ilmentymistasoon eli RNA:n tuottoon. Lis\u00e4ksi tutkittiin ko. deleetion vaikutusta D. montana -naaraiden lis\u00e4\u00e4ntymislepokauteen kasvattamalla deleetio- ja ei-deleetio-naaraita kasvatuskaapeissa valojaksoissa, joissa ne normaalisti siirtyv\u00e4t lis\u00e4\u00e4ntymislepokauteen (L:D 16:8) tai kehittyv\u00e4t sukukypsiksi (L:D 22:2). Vuonna 2003 luonnosta ker\u00e4ttyjen D. montana -yksil\u00f6iden joukosta (n=28) l\u00f6ytyi kaksi yksil\u00f6\u00e4, joilla deleetio esiintyi heterotsygoottisena. Sen sijaan vuonna 2008 ker\u00e4tyilt\u00e4 yksil\u00f6ilt\u00e4 (n=75) ei l\u00f6ytynyt lainkaan deleetiota. Toisen vuonna 2003 ker\u00e4tyn deleetion suhteen heterotsygoottisen naaraan j\u00e4lkel\u00e4isist\u00e4 koostuvassa laboratoriolinjassa deleetio on kuitenkin s\u00e4ilynyt ja on siin\u00e4 hyvin yleinen. T\u00e4ss\u00e4 linjassa cpo-geenin ilmentymistaso oli deleetioyksil\u00f6ill\u00e4 alhaisempi kuin ei-deleetioyksil\u00f6ill\u00e4 sek\u00e4 lis\u00e4\u00e4ntymislepokaudessa olevien ett\u00e4 sukukypsien naaraiden keskuudessa. Deleetio ei kuitenkaan vaikuttanut naaraiden lis\u00e4\u00e4ntymislepokauteen siirtymiseen, joten alue, jolla se sijaitsee, ei v\u00e4ltt\u00e4m\u00e4tt\u00e4 ole keskeinen D. montana -lajin lis\u00e4\u00e4ntymislepokauden m\u00e4\u00e4r\u00e4ytymisess\u00e4. On my\u00f6s mahdollista, ett\u00e4 ko. deleetioalue vaikuttaa lis\u00e4\u00e4ntymislepokauteen mutta deleetiosta huolimatta geenin transkriptiotuotetta syntyy riitt\u00e4v\u00e4 m\u00e4\u00e4r\u00e4 normaalin geenitoiminnan s\u00e4ilytt\u00e4miseksi eik\u00e4 deleetio siten vaikuta lis\u00e4\u00e4ntymislepokauteen siirtymiseen", "language": "", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Hanna Lampinen (hamalamp) on 2011-01-25 13:30:55.055260. Form: Pro gradu -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2011-01-25T13:30:58Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201101251168.doc: 424448 bytes, checksum: f676db37acdaf70209e280d289e6967a (MD5)\r\nlicense.html: 5057 bytes, checksum: 36460770a8d35128d84ac335a81a2970 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2011-01-25T13:30:58Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201101251168.doc: 424448 bytes, checksum: f676db37acdaf70209e280d289e6967a (MD5)\r\nlicense.html: 5057 bytes, checksum: 36460770a8d35128d84ac335a81a2970 (MD5)\r\n Previous issue date: 2010", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "26 sivua", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "cpo-geeni", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "diapaussi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Drosophila montana", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lis\u00e4\u00e4ntymislepokausi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Couch potato -kandidaattigeeniss\u00e4 esiintyv\u00e4n mutaation vaikutus Drosophila montana -naaraiden lis\u00e4\u00e4ntymislepokauteen", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201101251168", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ekologia ja evoluutiobiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ecology and evolutionary biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4011", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mutaatiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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