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[{"key": "dc.contributor.advisor", "value": "Laanto, Elina", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Oksanen, Hanna", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lepola, Kaisa", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2025-06-18T10:59:38Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2025-06-18T10:59:38Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2025", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/103739", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bakteriofagit eli faagit ovat viruksia, jotka infektoivat bakteereja. Ne ovat kaikista\nsuurilukuisin biologinen kokonaisuus maailmassa, ja niit\u00e4 esiintyy useissa\nerilaisissa ekosysteemeiss\u00e4. Vaikka faagien genomeista suurin osa on alle 200\nkiloem\u00e4sparia, my\u00f6s suurempia jumbofaageja sek\u00e4 megafaageja on eristetty tai\nkoottu metagenomiaineistosta. Megafaageja ei ole kuitenkaan saatu eristetty\u00e4,\nmink\u00e4 vuoksi niiden biologia tunnetaan huonosti. T\u00e4ss\u00e4 ty\u00f6ss\u00e4 tutkittiin\ntiett\u00e4v\u00e4sti ensimm\u00e4isen megafaagi-isolaatin, FKy-1:n vuorovaikutusta\nFlavobacterium sp. B222 -is\u00e4nt\u00e4bakteerin kanssa. Tutkimuksessa tarkasteltiin\nfaagin infektiivisyyden pysyvyytt\u00e4 eri olosuhteissa. Huomattiin, ett\u00e4 faagin\ninfektiivisyys laskee kloroformin vaikutuksesta, mutta se s\u00e4ilyy\nhuoneenl\u00e4mm\u00f6ss\u00e4 j\u00e4rvivedess\u00e4 yht\u00e4 hyvin kuin +4 \u00b0C:ssa kasvatusalustassa.\nT\u00e4m\u00e4n lis\u00e4ksi m\u00e4\u00e4ritettiin faagin elinkierto sek\u00e4 tutkittiin faagin\nvuorovaikutusta is\u00e4nt\u00e4bakteerin kanssa j\u00e4rvivedess\u00e4 ja mediassa. Elinkierron\nvaiheet kuvattiin elektronimikroskoopilla ohutleiken\u00e4ytteist\u00e4.\nElinkiertom\u00e4\u00e4rityksess\u00e4 merkitt\u00e4v\u00e4t muutokset bakteerin m\u00e4\u00e4r\u00e4ss\u00e4 n\u00e4kyiv\u00e4t\nnoin 3 tunnin j\u00e4lkeen infektiosta. Lis\u00e4ksi tutkimuksessa selvisi, ett\u00e4\nj\u00e4rvivesialusta sopii bakteeri-faagi vuorovaikutusten tarkasteluun, vaikkakin\nsolu- ja virusm\u00e4\u00e4r\u00e4t ovat j\u00e4rvivedess\u00e4 matalammat kuin mediassa. T\u00e4m\u00e4 l\u00f6yt\u00f6\ntukee hypoteesia siit\u00e4, ett\u00e4 j\u00e4rviveden ja median k\u00e4ytt\u00f6 kasvatusalustoina\neroavat toisistaan faagi-is\u00e4nt\u00e4-vuorovaikutusten suhteen. Lis\u00e4ksi havaittiin\nbakteerin pes\u00e4kemorfologian muutosta faagin ja j\u00e4rviveden vaikutuksesta. T\u00e4m\u00e4\ntulos antaa lis\u00e4\u00e4 tietoa megafaagien k\u00e4ytt\u00e4ytymisest\u00e4 luonnonymp\u00e4rist\u00f6ss\u00e4 ja\nluo pohjan mallille, jossa faagia ja is\u00e4nt\u00e4bakteeria voidaan kasvattaa niiden\nluonnollisessa ymp\u00e4rist\u00f6ss\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bacteriophages are viruses that infect bacteria and are known to be the most\nabundant biological entities in the world. They are found in a wide range of\necosystems. Most of the phages are less than 200 kilobase pairs, but larger\njumbophages and megaphages have been found. While megaphages have been\ndetected in metagenomic data, no isolates have been obtained. Because of that,\nbiology of megaphages remains poorly understood. In this study, we analysed\nthe interaction between the first known megaphage isolate, FKy-1, and its host,\nFlavobacterium sp. B222. We followed the stability of the infectivity of the\nmegaphage in different conditions. It was found that chloroform causes\ninfectivity loss on the phage. Also, we noticed that phage infectivity remains as\nstable in lake water at room temperature as in medium at +4 \u00b0C. We determined\nthe life cycle of the FKy-1 phage and observed differences in the phage-bacteria\ninteractions in lake water and in media. Stages of the phage life cycle was\ndetermined by electron microscopy from thin-slice-samples. Significant changes\nin the bacterial cell counts were observed approximately 3 hours after infection.\nAlso, lake water can be used as a growth medium, but it results in lower viral\nparticle and cell counts. In addition, it was found that lake water induces\nmorphological changes in the bacterial cells. This finding indicates that phagebacteria\ninteractions can be studied in lake water, but these limitations must be\nconsidered. In the future, it might be possible to study phage-bacteria\ninteractions in their natural environments.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2025-06-18T10:59:38Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2025-06-18T10:59:38Z (GMT). No. of bitstreams: 0\n Previous issue date: 2025", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "45", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "elinkiertom\u00e4\u00e4ritys", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "faagi-bakteeri vuorovaikutus", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "flavobakteeri", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "megafaagi", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Megafaagin ja sen is\u00e4nt\u00e4bakteerin vuorovaikutus j\u00e4rvivedess\u00e4", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202506185528", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": "fi", "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "vuorovaikutus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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