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[{"key": "dc.contributor.advisor", "value": "Gebraad, Arjen", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Mykuliak, Anastasiia", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Liedes, Jarkko", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2025-06-17T06:27:06Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2025-06-17T06:27:06Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2025", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/103640", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Rinta- ja eturauhassy\u00f6p\u00e4 ovat tunnettuja taipumuksestaan l\u00e4hett\u00e4\u00e4 et\u00e4pes\u00e4kkeit\u00e4\nluustoon. Molemmilla sy\u00f6vill\u00e4 on suuri esiintyvyys maailmanlaajuisesti, ja niihin\nliittyv\u00e4 kuolleisuus on suuri. Sy\u00f6p\u00e4tutkimuksessa k\u00e4ytet\u00e4\u00e4n in vivo-malleja\nkasvaimien ja metastaasiprosessien tutkimiseen. In vivo-mallien heikkoutena on\nkvantitatiivisen tutkimuksen ja kuvantamisen hankaluus. In vitro-mallit rajaavat\nuseita biologisia tekij\u00f6it\u00e4 pois, jonka vuoksi niit\u00e4 voidaan k\u00e4ytt\u00e4\u00e4 rajattujen\nprosessien kvantitatiiviseen tutkimiseen ja erilaisten prosessien kuvantamiseen.\nMikrofluidisia luumalleja voidaan k\u00e4ytt\u00e4\u00e4 metastaasiprosessien tutkimiseen.\nMalleissa yleisesti k\u00e4ytett\u00e4v\u00e4t fibriini- ja kollageeni-hydrogeelit ovat herkki\u00e4\nmenett\u00e4m\u00e4\u00e4n rakenteensa ja kutistumaan solujen ja soluv\u00e4liaineen\nvuorovaikutusten takia. T\u00e4m\u00e4n pro-gradun aiheena on tutkia kolmella eri\nkoevaiheella fibriini-, fibriini-hyaluronihappo-, kollageeni I-, ja kollageeni I-hyaluronihappo-\nhydrogeelien sopivuutta eri soluymp\u00e4rist\u00f6ihin Aim Biotech\nidenTx-3 mikrofluidisessa sirussa. Tutkimuksessa selvitettiin, mik\u00e4li\nhydrogeeleiss\u00e4 voidaan kasvattaa l\u00e4pivirtaava mikroverisuonitus, voidaanko\nhydrogeeleiss\u00e4 kasvattaa osteoklasti- ja osteoblasti-luusoluja, ja kest\u00e4v\u00e4tk\u00f6\nhydrogeelit mikroverisuonituksen tai luusolujen l\u00e4sn\u00e4olon kutistumatta tai\nmenett\u00e4m\u00e4tt\u00e4 rakennettaan. Mikroverisuonituksen l\u00e4pivirtaavuutta tutkittiin\nFluoSphere-mikrohelmill\u00e4, ja suonituksen morfologiaa tutkittiin vastaainev\u00e4rj\u00e4yksell\u00e4.\nLuusolujen vaikutusta hydrogeeleihin tutkittiin FluoSphere-mikrohelmill\u00e4,\nja niiden elinkelpoisuutta tutkittiin vasta-ainev\u00e4rj\u00e4yksell\u00e4.\nTutkimuksessa havaittiin, ett\u00e4 hyaluronihapon lis\u00e4\u00e4minen vaikuttaa solujen\nliikkuvuuteen ja elinkelpoisuuteen etenkin suurissa pitoisuuksissa, mutta my\u00f6s\nvahvistaa hydrogeelien rakennetta. Matalammat pitoisuudet eiv\u00e4t vahvistaneet\nhydrogeelien rakennetta, mutta s\u00e4ilyttiv\u00e4t solujen liikkumiskyky\u00e4 ja\nelinkelpoisuutta paremmin. Tulevissa tutkimuksissa tulisi keskitty\u00e4\nhydrogeelikoostumusten optimointiin solujen elinkelpoisuuden ja hydrogeelien\nrakenteellisen eheyden parantamiseksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bone metastasizing breast and prostate cancers are known for their high\nincidence and mortality globally. In vivo models are used for researching the\nprimary and secondary tumours and the complex metastatic process in a living\nbeing. However, visualizing the processes and gathering quantitative data\nremain a challenge. In vitro models allow the research of specific variables and\neasier visualization. Microfluidic bone-on-chip models are being developed for\nstudying bone metastases. Maintaining the structural integrity of the hydrogels\nwith embedded cells is currently a major challenge. The aim of this thesis was to\nstudy if: 1. the collagen I- and fibrin-hyaluronic acid composite hydrogels\nmaintain their size and shape in the presence of microvasculature, 2. do the\nhydrogels allow the formation of perfusable microvasculature and 3. do these\nhydrogels maintain their size and shape in the presence of embedded osteoblasts\nor osteoclasts in the hydrogels. The different hydrogels were tested on the\nidenTx-3 microfluidic chips by Aim Biotech. The perfusability of the microvasculature\nwas assessed by with FluoSphere microbeads and the morphology\nwas assessed using immunofluorescent imaging. The hydrogel shrinkage of\nembedded osteoclasts and osteoblasts was assessed by a microbead assay and\ntheir viability by immunofluorescent imaging. The activity of osteoclast specific\ntartrate resistant acid phosphatase was assessed in the conditioned medium from\nchips with hydrogel embedded osteoclasts and macrophages. We found that the\naddition of hyaluronic acid impacts cell migration and viability, especially at\nhigh concentrations, while increasing structural integrity. Lower concentrations\nof hyaluronic acid did not increase structural integrity but retained cell migration\nand viability. Future studies should aim to optimize hydrogel compounds for cell\nviability and hydrogel integrity.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2025-06-17T06:27:06Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2025-06-17T06:27:06Z (GMT). No. of bitstreams: 0\n Previous issue date: 2025", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "68", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "hBMSC", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "HUVEC", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "microfluidic chip", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "hydrogel contraction", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Use of collagen I-hyaluronic acid and fibrin-hyaluronic acid hydrogels for perfusable models of vascularized bone tissue", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202506175446", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": "fi", "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.yso", "value": "in vitro -menetelm\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikroverisuonet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "osteoblastit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "osteoklastit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrofluidistiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hydrogeelit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fibriini", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kudosteknologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biomateriaalit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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