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[{"key": "dc.contributor.advisor", "value": "Nissinen, Tuuli", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pekkala, Satu", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Kivel\u00e4, Riikka", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Tiensuu, Pihla", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2025-03-06T09:18:46Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2025-03-06T09:18:46Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2025", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/100589", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Johdanto: T\u00e4m\u00e4 pro-gradu tutkielma tutki suolistomikrobiston koostumuksen ja lepoaineenvaihdunnan v\u00e4list\u00e4 yhteytt\u00e4 liikuntaa harrastamattomilla ja harrastavilla naisilla. Suolistomikrobisto vaikuttaa ihmisen aineenvaihduntaan esimerkiksi anaerobisen lepoaineenvaihdunnan kautta (Riedl et al. 2017). Muutamat tutkimukset ovat antaneet viitteit\u00e4 siit\u00e4, ett\u00e4 tietyt mikrobit olisivat yhteydess\u00e4 lepoaineenvaihdunnan m\u00e4\u00e4r\u00e4\u00e4n (Bahr et al. 2015; Kocelak et al. 2013; Mehranfar et al. 2023). T\u00e4m\u00e4 tutkielma pyrki lis\u00e4\u00e4m\u00e4\u00e4n tietoa suolistomikrobiston koostumuksen ja lepoaineenvaihdunnan v\u00e4lisest\u00e4 suhteesta.\n\nMetodit: 12 tutkimukseen valikoitunutta normaalipainoista naishenkil\u00f6\u00e4 jaettiin liikuntaa har- rastamattomiin (n=4) ja liikuntaa harrastaviin (n=8) itse raportoidun liikunnan m\u00e4\u00e4r\u00e4n perusteella. Kaikilta tutkittavilta m\u00e4\u00e4ritettiin kehonkoostumus, lepoaineenvaihdunta sek\u00e4 maksimaalinen hapenottokyky. Lepoaineenvaihdunta normalisoitiin kehon rasvattomaan massaan. Tutkittavia ohjeistettiin ottamaan ulosten\u00e4yte itsen\u00e4isesti. Suolistomikrobiston koostumuksen tilastolliset analyysit suoritettiin CLC Microbial Genomics Package ohjelmalla ja muut tilastolliset analyysit IBM SPSS Statistics 29.0.2.0 ohjelmalla. Lepoaineenvaihdunnan ja suolistomikrobiston v\u00e4lisi\u00e4 korrelaatioita analysoitiin Spearmanin korrelaatiokertoimen avulla. Tilastolliseksi merkitsevyydeksi asetettiin p<0,05.\n\nTulokset: Suolistomikrobiston analyysiss\u00e4 tunnistettiin yhteens\u00e4 7 p\u00e4\u00e4jaksoa, 15 heimoa ja 73 sukua. Liikuntaa harrastamattomien ja liikuntaa harrastavien ryhmien v\u00e4lill\u00e4 havaittiin eroja kahden heimon osalta: Christensenellaceae (FDR<0,001) ja Tannerellaceae (FDR=0,01). Lis\u00e4ksi erottuivat kolme sukua: Christensenellaceae R-7 group (FDR=0,001), Parabacteroides (FDR=0,04) ja Ruminiclostridium 6 (FDR=0,04). Lepoaineenvaihdunnan ja mikrobiston v\u00e4lill\u00e4 ei l\u00f6ytynyt tilastollisesti merkitsevi\u00e4 yhteyksi\u00e4 ilman ryhm\u00e4jakoa. Kuitenkin liikuntaa harrastavilla havaittiin selke\u00e4 yhteys lepoaineenvaihdunnan ja p\u00e4\u00e4jaksojen Firmicutes (rho=0,731, p=0,004) ja Bacteroidetes (rho=-0,838, p=0,009) sek\u00e4 yhden suvun Bacteroides (rho=-0,778, p=0,023) v\u00e4lill\u00e4. Liikuntaa harrastamattomilla vastaavia yhteyksi\u00e4 ei l\u00f6ytynyt.\n\nJohtop\u00e4\u00e4t\u00f6kset: T\u00e4m\u00e4n tutkimuksen tulokset osoittavat, ett\u00e4 liikuntaa harrastavien lepoaineenvaihdunta oli positiivisesti yhteydess\u00e4 Firmicutes-p\u00e4\u00e4jakson mikrobien ja negatiivisesti Bacteroidetes-heimon ja Bacteroides-suvun mikrobien m\u00e4\u00e4r\u00e4n kanssa. Lepoaineenvaihdunnan ja suolistomikrobiston koostumuksen v\u00e4liset korrelaatiot olivat osittain yhdenmukaisia ja osittain ristiriitaisia aiemman tutkimustiedon kanssa. T\u00e4m\u00e4 tutkimus oli ensimm\u00e4inen, jossa otettiin liikunnan m\u00e4\u00e4r\u00e4 huomioon lepoaineenvaihdunnan ja suolistomikrobiston koostumuksen v\u00e4list\u00e4 yhteytt\u00e4 tutkittaessa. Tulevissa tutkimuksissa tulisi k\u00e4ytt\u00e4\u00e4 suurempia otoskokoja, ker\u00e4t\u00e4 tietoa tutkittavien ruokavaliosta ja viimeaikaisista liikuntatottumuksista ennen ulosten\u00e4ytteiden keruuta sek\u00e4 huomioida naisilla kuukautiskierron vaihe lepoaineenvaihdunnan mittauksissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Introduction: This master \u0301s thesis aimed to examine the relationship between gut microbiota and resting energy expenditure and to analyze whether the relationship differ between non-exercised and exercised subjects. Gut microbiota influences host \u0301s energy expenditure through anaerobic resting energy expenditure (Riedl et al. 2017). There is some evidence that specific bacterial groups could correlate with the amount of resting energy expenditure (Bahr et al. 2015; Kocelak et al. 2013; Mehranfar et al. 2023). This master \u0301s thesis aimed to increase knowledge about the relationship between gut microbiota and resting energy expenditure.\n\nMethods: 12 healthy normal weight women were accepted as subjects for this study. The subjects were divided into non-exercised group (n=4) and exercised group (n=8) based on a questionnaire about their physical activity status. Body composition, resting energy expenditure and maximal oxygen consumption were measured from all subjects. Resting energy expenditure was normalized to fat-free mass of the individual. Subjects were instructed to take the fecal samples independently. Statistical analyses concerning gut microbiota composition were conducted by using CLC Microbial Genomics Package. Rest of statistical analyses were carried out using IBM SPSS Statistics 29.0.2.0. Correlations between resting energy expenditure and gut microbiota were carried out by using Spearman\u2019s correlation coefficient. Statistical significance was set at p<0,05.\n\nResults: Gut microbiota composition was analyzed and a total of 7 phyla, 15 families and 73 genera were identified. Two families Christensenellaceae (FDR<0,001) and Tannerellaceae (FDR=0,01) as well as three genera Christensenellaceae R-7 group (FDR=0,001), Parabac- teroides (FDR=0,04) and Ruminiclostridium 6 (FDR=0,04) were significantly differing between the non-exercised and exercised groups. No significant correlations were found between gut microbiota and resting energy expenditure without group division. However, in the exercised group resting energy expenditure correlated positively with phylum Firmicutes (rho=0,731, p=0,004) and negatively with phylum Bacteroidetes (rho=-0,838, p=0,009) and genus Bacteroides (rho=-0,778, p=0,023). Resting energy expenditure values of the non-exercised subjects did not correlate with any of the examined gut microbiota.\n\nConclusion: The findings of this study indicate that resting energy expenditure of physically active individuals correlate positively with the abundance of Firmicutes and correlate negatively with the abundance of Bacteroidetes and Bacteroides. Correlations between resting energy expenditure and the examined gut microbiota were partly concordant and partly contradictory with previous research. This study was the first in which the physical activity status of the subjects was considered while studying the relationship between resting energy expenditure and the composition of gut microbiota. Future studies should include larger sample sizes, recorded diet and recent exercise habits before fecal sampling. They should also consider menstrual cycle phases when measuring resting energy expenditure in women. This thesis gives indicative results about the relationship between resting energy expenditure and gut microbiota composition.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2025-03-06T09:18:46Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2025-03-06T09:18:46Z (GMT). No. of bitstreams: 0\n Previous issue date: 2025", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "63", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "gut microbiota", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "resting energy expenditure", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "non-exercised", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "exercised", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The relationship between gut microbiota and resting energy expenditure in non-exercised and exercised women", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202503062398", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikunta- ja terveystieteet", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntafysiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Exercise Physiology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": "fi", "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.yso", "value": "naiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistomikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fyysinen aktiivisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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