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[{"key": "dc.contributor.advisor", "value": "Takala, Heikki", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Lehtinen, Kimmo", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Rantala, Vaula", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2025-02-19T06:50:56Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2025-02-19T06:50:56Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2025", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/100264", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetiikka on tekniikka, jossa geeniteknologia yhdistettyn\u00e4 valokontrolliin mahdollistaa biologisten prosessien ajallisesti ja paikallisesti tarkan s\u00e4\u00e4telyn. Optogeneettisiss\u00e4 sovelluksissa hy\u00f6dynnet\u00e4\u00e4n valoaktiivisia proteiineja, kuten fytokromeja, solun toimintojen manipuloimiseksi. Optogeneettisill\u00e4 ty\u00f6kaluilla valolla voidaan hallita esimerkiksi geenien ilment\u00e4mist\u00e4 bakteereissa tai nis\u00e4kk\u00e4iss\u00e4. Potentiaalisia k\u00e4ytt\u00f6kohteita bakteereissa ovat muuan muassa synteettinen biologia ja biomateriaalien tuotannon sek\u00e4 metabolisten prosessien tehostaminen, nis\u00e4k\u00e4ssoluissa taas erityisesti l\u00e4\u00e4ketieteelliset sovellukset. In vivo -kokeita ja nis\u00e4k\u00e4ssolusovelluksia ajatellen kiinnostavia ovat punaisen/kaukopunaisen valon aallonpituuksilla hallittavat ty\u00f6kalut, jotka ovat soluille turvallisempia ja mahdollistavat valon p\u00e4\u00e4syn syvemm\u00e4lle kudoksiin. pREDusk on punaista valoa aistivaan bakteerifytokromiin perustuva ty\u00f6kalu halutun geenin ilment\u00e4misen s\u00e4\u00e4telyyn bakteereissa. T\u00e4m\u00e4n tutkimuksen tavoitteena oli l\u00f6yt\u00e4\u00e4 pREDusk-ty\u00f6kalun toiminnalle t\u00e4rke\u00e4t promoottorialueen osat ja sekvenssielementit ja lyhent\u00e4\u00e4 promoottorialuetta mahdollisia tulevia nis\u00e4k\u00e4ssolusovelluksia varten. Hypoteesina oli, ett\u00e4 alkuper\u00e4ist\u00e4 lyhyempi alue riitt\u00e4\u00e4 takaamaan toiminnan bakteereissa. Promoottorialueesta valmistettiin mutageneesill\u00e4 lyhennettyj\u00e4 variantteja, joihin sis\u00e4llytettiin erilaisia yhdistelmi\u00e4 transkription aloitukselle aiemmin t\u00e4rkeiksi tunnistetuista sitoutumiskohdista ja muista promoottorisekvenssin elementeist\u00e4. Varianttien toimintatehokkuutta bakteereissa arvioitiin fluoresenssimittauksilla. Ty\u00f6kalun havaittiin s\u00e4ilytt\u00e4v\u00e4n toimintansa bakteereissa my\u00f6s lyhennettyn\u00e4 versiona. Ty\u00f6n t\u00e4rkeimp\u00e4n\u00e4 tuloksena tunnistettiin promoottorialueen sekvenssielementit, jotka ovat t\u00e4rkeit\u00e4 ty\u00f6kalun toiminnalle bakteereissa. Tuloksia voidaan hy\u00f6dynt\u00e4\u00e4 tulevissa tutkimuksissa, joiden my\u00f6t\u00e4 pREDusk-ty\u00f6kalun potentiaali voidaan siirt\u00e4\u00e4 k\u00e4yt\u00e4nn\u00f6n sovelluksiin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Optogenetics is a technique combining genetic engineering and light-based control to regulate biological processes with high precision. By utilising light-sensitive proteins, e. g. phytochromes, optogenetic applications allow manipulating cellular activities such as gene expression by light in both prokaryotes and eukaryotes. Potential optogenetic applications for bacterial use are for example developing synthetic genetic circuits and controlled production of metabolites and biofuels. In mammalian use, optogenetic tools have potential for various therapeutic applications. Interesting tools considering in vivo mammalian applications are ones sensitive to red/far-red light which is less phototoxic and enables deeper tissue penetration compared to shorter wavelengths. pREDusk is an optogenetic tool based on a red light-absorbing bacteriophytochrome for controlling bacterial gene expression. The aim of this study was to identify the essential promoter sequence elements for the tool\u00b4s function in bacteria and consider the potential of a shortened sequence for mammalian applications. Hypothesis was that a shortened promoter area would be sufficient for function in bacteria. Promoter variants with different combinations of previously determined binding sites and other sequence elements were created by mutagenesis, and their functionality in bacteria was assessed by measuring target protein fluorescence. A shortened variant with effective function was created successfully, and sequence elements essential for function in bacteria were located and identified. The results can benefit future studies, through which the potential of the pREDusk tool can be transferred to practical applications.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2025-02-19T06:50:56Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2025-02-19T06:50:56Z (GMT). No. of bitstreams: 0\n Previous issue date: 2025", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "54", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "promoter engineering", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "red light", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "two-component system", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Mapping promoter elements of a bacterial gene expression tool for improved optogenetic applicability", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202502192042", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": null, "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniekspressio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fytokromit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniekspressio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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